FastQCFastQC Report
Thu 26 May 2016
SRR1512125_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512125_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3485902
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT55710.15981516405223095No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCGA350.002241455516.20794110
GGTATCA20000.011.4387291
AGGACGT12500.010.6648245
GTAGGAC13500.010.6452153
ATACCGT1002.5309146E-510.4000956
TAGGACC5000.010.4000944
GACGTGA7250.010.3022887
GTCCTAA7900.010.15438651
GGACGTG13300.010.0944196
TAGGACG13200.010.0276394
GTATCAA37650.09.6800731
TTAGGAC8850.09.614883
TTAGACT3050.09.6096264
CTGTAGG14900.09.5048561
TGTAGGA14600.09.4966612
GGCGATA800.0046991889.45476719
GGCGAGG9050.09.4025319
TAGGACA6700.09.3135184
GTACCGT1951.8371793E-99.2122056
GTATTAA4000.09.1608881
Produced by FastQC (version 0.11.5)