FastQCFastQC Report
Thu 26 May 2016
SRR1512125_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512125_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3485902
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT74050.21242708486928205No Hit
TATCAACGCAGAGTACTTTTTTTTT48080.13792699852147305No Hit
GGTATCAACGCAGAGTACTTTTTTT47380.13591890994066957No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21850.012.3988671
GTCCTAA7550.010.992271
GGACGTG14050.010.8826696
AGGACGT14650.010.5719215
GACGTGG7850.010.4043447
TAGGACG15100.010.3827154
GTAGGAC14800.010.2721713
TGTAGGA16100.010.1509472
TTAGGAC10050.09.83266453
TAGGACC5850.09.7453934
CGTGAAA8700.09.7148919
GTATCAA43350.09.6823031
TTAGGCA2600.09.5017584
GACGTGA7500.09.4969887
CGTGGAA8200.09.4965799
ACGTGGA8700.09.3878278
GTATTAG1752.4994733E-89.2667191
ATTAGGC1651.06381776E-79.2138263
GTCCGAT850.00746063248.93795711
ACGTGAA9250.08.9323028
Produced by FastQC (version 0.11.5)