FastQCFastQC Report
Thu 26 May 2016
SRR1512124_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512124_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3242156
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT49680.1532313682623538No Hit
GTCCTACAGTGGACATTTCTAAATT32490.10021109409911182No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTC701.14111805E-412.1563677
TAGGACC7500.011.7241424
GGTATCA16300.011.5410011
TCCAACG2800.011.14333618
CGTACAT1001.5199725E-510.8910612
GTCCTAG4550.010.227371
GTATCAA31050.010.1082311
GTCCTAC18400.010.0623921
GTATTAG2700.09.9009641
CTTATAC2600.09.8186043
TTAGGAC13400.09.737193
TCCTACA20450.09.731512
TAGGACA6350.09.6782984
AGCTCGC1304.5065663E-69.45495310
GGACCGT1502.8402792E-79.4549526
CCTACAG22000.09.4119763
GTCCTAA10850.09.3990721
GTCTACG850.00529573539.3185541
GGCGAGG9650.09.30798419
GGACGTG19450.09.2362016
Produced by FastQC (version 0.11.5)