FastQCFastQC Report
Thu 26 May 2016
SRR1512124_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512124_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3242156
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT62970.19422260989292311No Hit
TATCAACGCAGAGTACTTTTTTTTT41320.12744605750000926No Hit
GGTATCAACGCAGAGTACTTTTTTT40430.12470097058870702No Hit
GTCCTACAGTGGACATTTCTAAATT36890.1137823102898195No Hit
GTCCTAAAGTGTGTATTTCTCATTT33140.10221593285455728No Hit
CTGTAGGACGTGGAATATGGCAAGA33130.10218508918139657No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGAT550.00307756712.0867687
GGTATCA19650.011.79719451
CATTTCG752.0823488E-411.39574414
TAGGACC7250.010.2228644
GTCTAAC1605.7716534E-910.1359391
CGTGCGC1502.462184E-810.1295510
GGACGTG18150.010.0984696
CCGTCCA856.619746E-410.0550689
TACGTCT951.6415458E-410.0021284
TACACCG1356.430346E-79.8539485
AGGACGT19400.09.8448785
GGCGAGG9200.09.70379619
GTATCAA36750.09.6046021
TTAGGAC12300.09.5020213
GACGTGG9900.09.4967467
TAGGACG20750.09.4333324
GTCCTAA12650.09.2003491
GGACCGT1554.2855208E-79.1903996
GACGTGA10550.09.1816887
TGGCGAG19500.09.15583718
Produced by FastQC (version 0.11.5)