##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512124_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3242156 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.259122324774008 31.0 31.0 34.0 28.0 34.0 2 31.447055601272734 33.0 31.0 34.0 28.0 34.0 3 31.551580799936833 33.0 31.0 34.0 28.0 34.0 4 35.1197767781686 37.0 35.0 37.0 32.0 37.0 5 34.786998219703186 37.0 35.0 37.0 32.0 37.0 6 34.74402989862301 37.0 35.0 37.0 32.0 37.0 7 34.5965925143639 37.0 35.0 37.0 31.0 37.0 8 34.51615406538119 37.0 35.0 37.0 31.0 37.0 9 36.080545476528584 39.0 37.0 39.0 31.0 39.0 10 35.911886411387975 39.0 35.0 39.0 30.0 39.0 11 35.90481303182204 39.0 35.0 39.0 30.0 39.0 12 35.75275588219691 39.0 35.0 39.0 30.0 39.0 13 35.726603840160685 39.0 35.0 39.0 30.0 39.0 14 36.83476951756794 40.0 37.0 41.0 30.0 41.0 15 36.77841750982988 40.0 36.0 41.0 30.0 41.0 16 36.69068854182217 40.0 36.0 41.0 30.0 41.0 17 36.63314751048377 40.0 36.0 41.0 30.0 41.0 18 36.57101169715461 40.0 36.0 41.0 30.0 41.0 19 36.58967736284127 40.0 36.0 41.0 30.0 41.0 20 36.489423396036464 40.0 36.0 41.0 30.0 41.0 21 36.38221109656661 40.0 36.0 41.0 30.0 41.0 22 36.27927249644989 40.0 36.0 41.0 30.0 41.0 23 36.34808812407546 40.0 36.0 41.0 30.0 41.0 24 36.25722605574809 40.0 36.0 41.0 30.0 41.0 25 35.85112036558389 39.0 36.0 41.0 28.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.08328180983146538 1101 2 -0.04280154517677204 1101 3 0.06476725310487907 1101 4 -0.018839638293989935 1101 5 0.038744684041581934 1101 6 -0.09751913001964851 1101 7 -0.07702512425764496 1101 8 -0.07863294325726855 1101 9 -0.02324383588018719 1101 10 -0.003948900046438553 1101 11 -0.025526545524691358 1101 12 -0.34538606972223107 1101 13 -0.11738412236591245 1101 14 -0.17970658057976863 1101 15 -0.15840987507951354 1101 16 -0.22933340839470873 1101 17 -0.13980658795239975 1101 18 -0.20481298208129317 1101 19 -0.23281992405579643 1101 20 -0.11975383455830979 1101 21 -0.13660501010570414 1101 22 -0.17934688435178003 1101 23 -0.25485747059159536 1101 24 -0.4469980350976783 1101 25 -0.5267160409675782 1102 1 0.05690258117610725 1102 2 0.09173671145378037 1102 3 0.1129294397395384 1102 4 0.10590214129475584 1102 5 0.026369477412615083 1102 6 0.04718411392362043 1102 7 0.11485564687642125 1102 8 0.11726795906644583 1102 9 0.19099687502877316 1102 10 0.16976494646377205 1102 11 0.17085742111986946 1102 12 0.163688969497791 1102 13 0.2359574234820272 1102 14 0.23045461634158926 1102 15 0.11049901865265355 1102 16 0.07649495004996254 1102 17 0.1994416510949506 1102 18 0.13882158207634632 1102 19 0.11857125486120879 1102 20 0.22697305721548844 1102 21 0.2514314618822482 1102 22 0.28085253234910823 1102 23 0.3268056689053367 1102 24 0.06079964223473411 1102 25 0.12529149769967773 1103 1 0.16637171901357917 1103 2 0.20967963729416894 1103 3 0.23698454369585065 1103 4 0.2418523425234227 1103 5 0.3516247508549739 1103 6 0.20073773384806515 1103 7 0.2749888897458561 1103 8 0.26541000220472455 1103 9 0.36269184047407066 1103 10 0.2983184480607761 1103 11 0.1859907802588694 1103 12 0.3416455143118853 1103 13 0.2189648830495443 1103 14 0.3080686701017541 1103 15 0.24137256119301043 1103 16 0.36591758829088405 1103 17 0.39644356306057205 1103 18 0.48090744170048794 1103 19 0.33604062400309687 1103 20 0.42617112644094846 1103 21 0.5443643093116748 1103 22 0.5342331472690205 1103 23 0.4412196589538979 1103 24 0.5611424831263108 1103 25 0.4337981910198536 1104 1 0.027450942806218848 1104 2 0.08653782739445148 1104 3 0.06993749270134941 1104 4 0.07537247277994652 1104 5 0.05694320683026888 1104 6 0.04108858544989147 1104 7 0.0605423667993108 1104 8 0.05787991156940109 1104 9 0.11165438065737732 1104 10 0.09097144892543696 1104 11 0.21958656150879108 1104 12 0.24974273589837992 1104 13 0.15547327784511822 1104 14 0.2807607740796172 1104 15 0.2984514369106037 1104 16 0.3845214954836109 1104 17 0.2614667990821147 1104 18 0.2530242158495781 1104 19 0.4408522585625221 1104 20 0.42672633632466983 1104 21 0.4435051373580734 1104 22 0.27752729533270326 1104 23 0.36453208598766196 1104 24 0.2872840762336182 1104 25 0.36186053468159685 1105 1 0.038090436274085704 1105 2 -0.024777562825860855 1105 3 -0.10810527624773769 1105 4 -0.08316476573462239 1105 5 0.02884728688204774 1105 6 -0.0990185021963228 1105 7 0.010904459477977468 1105 8 -0.03050375672621186 1105 9 -0.08145711571930292 1105 10 -0.05186097476491369 1105 11 -0.13274396249443043 1105 12 -0.24114178391504026 1105 13 -0.20523426518946764 1105 14 -0.05115947882236327 1105 15 -0.05348453853119395 1105 16 -0.1389480599402546 1105 17 0.10137301954546274 1105 18 -0.22632315982283302 1105 19 -0.2131053710610047 1105 20 0.0086391878482317 1105 21 -0.08721987676203469 1105 22 -0.07489329075107776 1105 23 -0.1365524435026444 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0.06891711792011534 1107 10 0.10314498967040464 1107 11 -0.007454016960359411 1107 12 0.10874706490270825 1107 13 -0.02492013826258699 1107 14 0.20367642985242185 1107 15 0.17599151587925377 1107 16 0.22959356626996907 1107 17 0.09713827046786605 1107 18 0.15847067415317895 1107 19 0.15455712512453346 1107 20 0.19700225088629963 1107 21 0.12222261036125559 1107 22 0.09795068147037256 1107 23 0.01390556964685885 1107 24 -0.0236150246654816 1107 25 -0.05834544981010481 1108 1 0.05331109071605766 1108 2 -9.709119776424302E-4 1108 3 0.007508675924704278 1108 4 0.03498421406870733 1108 5 0.09143139784020349 1108 6 0.09841988097994658 1108 7 -0.02657473801966148 1108 8 0.007518161518206057 1108 9 0.04479896814979156 1108 10 0.009218058786949257 1108 11 0.03811661295809898 1108 12 0.050489756210616576 1108 13 -0.0076961949516842765 1108 14 0.036636583682138735 1108 15 0.1624687440956123 1108 16 0.22348738103335108 1108 17 0.21381383931735343 1108 18 0.16360296058981305 1108 19 0.20885794768622645 1108 20 0.198862451007372 1108 21 0.13184907477032937 1108 22 0.31082822304357194 1108 23 0.30519068875806 1108 24 0.24597262622651783 1108 25 0.3006030243557589 1109 1 -0.2391644508573414 1109 2 -0.21182219052094098 1109 3 -0.2652653448069664 1109 4 -0.274624586562517 1109 5 -0.2329830052861439 1109 6 -0.2017767007665654 1109 7 -0.15796432206555266 1109 8 -0.14394782018834462 1109 9 -0.22250022554334237 1109 10 -0.3855372214901962 1109 11 -0.2902659984466922 1109 12 -0.2727768194742026 1109 13 -0.250703912605438 1109 14 -0.196341909157276 1109 15 -0.2952048861817005 1109 16 -0.31125526789298874 1109 17 -0.2438232176117765 1109 18 -0.2560162702184883 1109 19 -0.21152356651811743 1109 20 -0.2446945441534325 1109 21 -0.37303719227606535 1109 22 -0.40403342472122716 1109 23 -0.18383087258526132 1109 24 -0.25031924263771543 1109 25 -0.1838323068531551 1110 1 -0.296255261703557 1110 2 -0.23360631417826383 1110 3 -0.22252317163431456 1110 4 -0.1570428797246919 1110 5 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14 -0.27739662729320713 2314 15 -0.3480575122893015 2314 16 -0.3248762115509294 2314 17 -0.2999316819916231 2314 18 -0.3920108756026508 2314 19 -0.411323344459241 2314 20 -0.43398866143865433 2314 21 -0.3365649450218129 2314 22 -0.3766497518920673 2314 23 -0.33623123058599447 2314 24 -0.19989619280364224 2314 25 -0.26095209254481233 2315 1 -0.5318899257931307 2315 2 -0.626181627428064 2315 3 -0.6202046232472185 2315 4 -0.5834200124892845 2315 5 -0.7190116301216349 2315 6 -0.7830165929912738 2315 7 -0.6338507139364609 2315 8 -0.6739957536116705 2315 9 -0.9555616877317163 2315 10 -0.8191424003589205 2315 11 -0.7694143830849498 2315 12 -0.7551016865413516 2315 13 -0.8045833391867845 2315 14 -0.9694267426349157 2315 15 -0.9456125008274014 2315 16 -1.1748479160014256 2315 17 -1.130147786583585 2315 18 -1.2010485651607823 2315 19 -1.122246393937047 2315 20 -1.117350366193591 2315 21 -0.9679364583315362 2315 22 -1.0127007003833555 2315 23 -1.0640683039868435 2315 24 -1.0621354192269337 2315 25 -1.084707308869909 2316 1 -0.8057634450143105 2316 2 -0.8582004495200621 2316 3 -0.9197374446371391 2316 4 -0.7077236502360691 2316 5 -0.9730227352852054 2316 6 -1.0561783377182294 2316 7 -1.020943649800195 2316 8 -1.1505174645182308 2316 9 -1.276376229803141 2316 10 -1.446729863942771 2316 11 -1.3787266829993783 2316 12 -1.3951459445043213 2316 13 -1.3530979762535083 2316 14 -1.521132176544974 2316 15 -1.456289286588465 2316 16 -1.7109547815680983 2316 17 -1.6116828276055628 2316 18 -1.2084442186495608 2316 19 -1.359615645777211 2316 20 -1.3830615087101705 2316 21 -1.5212857748293729 2316 22 -1.5675666285329157 2316 23 -1.4196410100679486 2316 24 -1.373485515377098 2316 25 -1.6284798577949786 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 35481.0 3 1.0 4 659.0 5 5922.0 6 7740.0 7 6186.0 8 4938.0 9 4742.0 10 4485.0 11 4149.0 12 4058.0 13 3911.0 14 3856.0 15 4039.0 16 4130.0 17 4377.0 18 4649.0 19 5396.0 20 6448.0 21 8339.0 22 10887.0 23 13838.0 24 16854.0 25 20766.0 26 24387.0 27 27874.0 28 33561.0 29 40920.0 30 52870.0 31 68959.0 32 90215.0 33 118690.0 34 156821.0 35 209991.0 36 304271.0 37 531439.0 38 1396307.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.22654930864726 19.054877576501134 13.567708575376503 25.15086453947511 2 15.169226897163494 20.487293023531254 39.891510464024556 24.451969615280696 3 18.4363121330374 24.654119049175918 30.098551704483068 26.811017113303613 4 12.958876747448302 17.31862994871314 37.48298971425187 32.23950358958668 5 13.743646844647087 37.34018163026542 35.39426210814429 13.52190941694321 6 31.989793211677664 36.789284661194586 18.027880213043417 13.193041914084331 7 28.225353746087478 31.488768584855265 22.166484277746044 18.119393391311213 8 25.666974087223128 36.03970351909143 20.460267178280276 17.833055215405164 9 26.5587158668491 15.409900078836428 19.885903084243942 38.14548097007054 10 15.417672684472924 28.711079911022168 33.80923064775415 22.062016756750754 11 34.39877661654776 22.382297458851454 24.24405241450442 18.97487351009637 12 23.314732542172553 24.342505419233373 31.30429257568112 21.03846946291295 13 28.82298075724919 21.5743782840801 25.85797845631117 23.74466250235954 14 21.63171667248584 21.432559074887205 27.25306863704276 29.682655615584196 15 23.43856988991276 29.701130975807455 23.40936093142958 23.450938202850203 16 22.957100151874247 27.671864031218735 26.74704733516833 22.623988481738692 17 22.28939631529143 27.90689282070326 27.28989598279663 22.513814881208678 18 22.147638793444855 27.33572968111343 28.685417974952472 21.83121355048924 19 23.81661933185119 26.682154322190936 27.07093930204383 22.430287043914042 20 23.85036377028126 26.89864398875316 27.454076855031033 21.796915385934547 21 23.697533369769992 26.33207655646428 26.852871977782684 23.117518095983044 22 23.68192647115068 26.882358529324314 26.993241534337027 22.44247346518798 23 23.29104460118514 26.80620550029055 27.0123337680235 22.890416130500814 24 23.45305748782853 26.458818115192805 27.42951282044955 22.658611576529115 25 23.421348248759603 26.586794616578995 27.310214175864918 22.681642958796484 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 463.0 1 463.0 2 572.0 3 681.0 4 681.0 5 681.0 6 1621.5 7 2562.0 8 2562.0 9 2562.0 10 3813.0 11 5064.0 12 5064.0 13 5064.0 14 8753.5 15 12443.0 16 12443.0 17 12443.0 18 23036.5 19 33630.0 20 33630.0 21 33630.0 22 53646.5 23 73663.0 24 73663.0 25 73663.0 26 106372.0 27 139081.0 28 139081.0 29 139081.0 30 180288.5 31 221496.0 32 221496.0 33 221496.0 34 253280.5 35 285065.0 36 285065.0 37 285065.0 38 313761.0 39 342457.0 40 342457.0 41 342457.0 42 369004.0 43 395551.0 44 395551.0 45 395551.0 46 407414.0 47 419277.0 48 419277.0 49 419277.0 50 415364.5 51 411452.0 52 411452.0 53 411452.0 54 375862.0 55 340272.0 56 340272.0 57 340272.0 58 293554.0 59 246836.0 60 246836.0 61 246836.0 62 203118.0 63 159400.0 64 159400.0 65 159400.0 66 124258.5 67 89117.0 68 89117.0 69 89117.0 70 64785.0 71 40453.0 72 40453.0 73 40453.0 74 28172.0 75 15891.0 76 15891.0 77 15891.0 78 10568.0 79 5245.0 80 5245.0 81 5245.0 82 3398.5 83 1552.0 84 1552.0 85 1552.0 86 966.5 87 381.0 88 381.0 89 381.0 90 240.5 91 100.0 92 100.0 93 100.0 94 60.0 95 20.0 96 20.0 97 20.0 98 12.0 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.44495082901624716 2 0.0 3 0.0 4 0.0 5 0.0566598275962045 6 0.0 7 0.0 8 0.0038863028182481037 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 1.2337469264279695E-4 20 0.0 21 0.0 22 0.0 23 0.0 24 0.004564863627783487 25 0.0016655583506777591 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3242156.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.6989331625598 #Duplication Level Percentage of deduplicated Percentage of total 1 78.94263755453328 51.86447068371465 2 13.352683583795521 17.545141326251827 3 3.809544251211023 7.508489794203807 4 1.508121755403282 3.963279616369703 5 0.7440866665029742 2.444285008486541 6 0.43652030971623534 1.7207351191288107 7 0.2615332554114896 1.2027719106946297 8 0.17819039552922383 0.9365535108867655 9 0.12909892991793162 0.7633495771232562 >10 0.5952497484627104 6.606214655900956 >50 0.025430593308196123 1.1386935930953976 >100 0.013901247178678779 1.7493227287134705 >500 0.001594657924512734 0.7337410372191423 >1k 0.001360149401468736 1.6289161913142718 >5k 4.6901703498921935E-5 0.19403524689680815 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6297 0.19422260989292311 No Hit TATCAACGCAGAGTACTTTTTTTTT 4132 0.12744605750000926 No Hit GGTATCAACGCAGAGTACTTTTTTT 4043 0.12470097058870702 No Hit GTCCTACAGTGGACATTTCTAAATT 3689 0.1137823102898195 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3314 0.10221593285455728 No Hit CTGTAGGACGTGGAATATGGCAAGA 3313 0.10218508918139657 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 3.084367316069924E-5 0.0 9 0.0 0.0 0.0 6.168734632139847E-5 0.0 10 0.0 0.0 0.0 6.168734632139847E-5 0.0 11 0.0 0.0 0.0 6.168734632139847E-5 0.0 12 0.0 0.0 0.0 6.168734632139847E-5 0.0 13 0.0 0.0 0.0 6.168734632139847E-5 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACCGAT 55 0.003077567 12.086768 7 GGTATCA 1965 0.0 11.7971945 1 CATTTCG 75 2.0823488E-4 11.395744 14 TAGGACC 725 0.0 10.222864 4 GTCTAAC 160 5.7716534E-9 10.135939 1 CGTGCGC 150 2.462184E-8 10.12955 10 GGACGTG 1815 0.0 10.098469 6 CCGTCCA 85 6.619746E-4 10.055068 9 TACGTCT 95 1.6415458E-4 10.002128 4 TACACCG 135 6.430346E-7 9.853948 5 AGGACGT 1940 0.0 9.844878 5 GGCGAGG 920 0.0 9.703796 19 GTATCAA 3675 0.0 9.604602 1 TTAGGAC 1230 0.0 9.502021 3 GACGTGG 990 0.0 9.496746 7 TAGGACG 2075 0.0 9.433332 4 GTCCTAA 1265 0.0 9.200349 1 GGACCGT 155 4.2855208E-7 9.190399 6 GACGTGA 1055 0.0 9.181688 7 TGGCGAG 1950 0.0 9.155837 18 >>END_MODULE