FastQCFastQC Report
Thu 26 May 2016
SRR1512123_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512123_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2880329
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT55200.19164477391298007No Hit
TATCAACGCAGAGTACTTTTTTTTT32820.11394531666347837No Hit
GGTATCAACGCAGAGTACTTTTTTT31870.11064708232983106No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTGCG655.7024135E-513.0919549
TATACCG803.0101497E-511.8191255
GGTATCA14100.011.793041
TAGGACC5350.011.4877474
AGGACGT9300.010.268665
GTACTAG1957.4578566E-1110.1515181
GTATCAA27950.010.0924981
GGACGTG9100.09.9748226
GTAGGAC12350.09.9529483
ATAAGAC2400.09.8492713
TGTAGGA13200.09.8227472
GTACTAC2550.09.7036571
TGGCGAG9200.09.6608518
CATTGCG800.00469658769.45539
GGATCGT800.00469658769.45536
AGAACCG1201.7990855E-59.4552995
TAGGACG10900.09.2818084
TTAGACA3700.09.1997524
GTCCTAT2800.09.1907491
ATTATAC2900.09.1292553
Produced by FastQC (version 0.11.5)