FastQCFastQC Report
Thu 26 May 2016
SRR1512123_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512123_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2880329
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69450.2411182889176896No Hit
TATCAACGCAGAGTACTTTTTTTTT43870.15230898970221807No Hit
GGTATCAACGCAGAGTACTTTTTTT42270.1467540687192331No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAA400.005142051314.3071981
GGTATCA15550.012.2063541
TAGGACC4000.011.1654494
GTATCAA35550.010.83939551
GTCCTAC9300.010.4611771
TGGATCG750.00264294610.136015
GGATCGT750.00265630510.1298526
GGACGTG10600.010.0342876
GCGTGCA1054.1246232E-59.9486169
CCAACGA1151.0308906E-59.90998219
TCCAACG1554.0867235E-89.80342318
TAGACAG5050.09.7847625
AGGACGT11200.09.7570415
TAAGACA4200.09.7287614
TCTAAAC3550.09.6363493
GTATTAT3200.09.5381321
TGGACCG1502.6402267E-79.502515
CGTAGAG900.00111143239.502512
TAGGACA4800.09.502514
ACCGTAT800.00453024549.4967378
Produced by FastQC (version 0.11.5)