##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512123_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2880329 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.067760314880697 31.0 31.0 34.0 28.0 34.0 2 31.25087516044174 31.0 31.0 34.0 28.0 34.0 3 31.35604995123821 33.0 31.0 34.0 28.0 34.0 4 34.942150011335514 37.0 35.0 37.0 32.0 37.0 5 34.58402390837991 37.0 35.0 37.0 32.0 37.0 6 34.537458741692355 37.0 35.0 37.0 31.0 37.0 7 34.37897441576987 37.0 35.0 37.0 30.0 37.0 8 34.291200067770035 37.0 35.0 37.0 30.0 37.0 9 35.82127840257137 39.0 35.0 39.0 30.0 39.0 10 35.64178154648306 39.0 35.0 39.0 30.0 39.0 11 35.64041468873868 39.0 35.0 39.0 30.0 39.0 12 35.46431536119658 39.0 35.0 39.0 30.0 39.0 13 35.44180716855609 39.0 35.0 39.0 30.0 39.0 14 36.50202494228958 40.0 36.0 41.0 30.0 41.0 15 36.43644597544239 40.0 36.0 41.0 30.0 41.0 16 36.339162644267375 40.0 36.0 41.0 29.0 41.0 17 36.27389614172548 40.0 36.0 41.0 29.0 41.0 18 36.206132355019164 39.0 36.0 41.0 29.0 41.0 19 36.22156635578783 40.0 36.0 41.0 29.0 41.0 20 36.10662497235559 39.0 36.0 41.0 28.0 41.0 21 36.001218610790644 39.0 36.0 41.0 28.0 41.0 22 35.885353721744984 39.0 36.0 41.0 27.0 41.0 23 35.95851515573395 39.0 36.0 41.0 29.0 41.0 24 35.85237311432132 39.0 36.0 41.0 28.0 41.0 25 35.42997449249721 39.0 35.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.05896874088381665 1101 2 0.02666895691348614 1101 3 0.02895856746579284 1101 4 -0.14425646208176346 1101 5 0.018372087948982596 1101 6 -0.10122109682203728 1101 7 -0.12646077753559837 1101 8 -0.014050666969275483 1101 9 -0.15050648938033362 1101 10 0.0018712158246785293 1101 11 -0.037803931675973956 1101 12 -0.172168281095324 1101 13 -0.15238573100531028 1101 14 -0.2122531179387721 1101 15 -0.05534204609811866 1101 16 -0.1896609632634707 1101 17 -0.18273646378766273 1101 18 -0.1813024744395335 1101 19 -0.3683185083620799 1101 20 -0.35462911880901515 1101 21 -0.32869150615596254 1101 22 -0.5575042247775315 1101 23 -0.4521460272484106 1101 24 -0.6908915563967852 1101 25 -0.6168068032005181 1102 1 0.1219106679857731 1102 2 0.14856151939026319 1102 3 0.21614099432818534 1102 4 0.17069381515364057 1102 5 0.1783197866881281 1102 6 0.06714744907277748 1102 7 0.241560410766688 1102 8 0.30579418230296795 1102 9 0.29194706676662463 1102 10 0.25853026978811755 1102 11 0.2289300788240709 1102 12 0.43190926523022455 1102 13 0.3885721628767982 1102 14 0.48744684619350664 1102 15 0.4794866089270471 1102 16 0.34076058726510894 1102 17 0.5436848146409972 1102 18 0.41294062263829545 1102 19 0.282762013316912 1102 20 0.38978100244752767 1102 21 0.40697390989072346 1102 22 0.4161028898286929 1102 23 0.4569552579128455 1102 24 0.4058156312892365 1102 25 0.35015281900003714 1103 1 0.22003216471309273 1103 2 0.2629497598753403 1103 3 0.2869632861474827 1103 4 0.2626836354225901 1103 5 0.45431679228615707 1103 6 0.3550801018009224 1103 7 0.40276115257108813 1103 8 0.4358045669466648 1103 9 0.4133941104897332 1103 10 0.22487104982570827 1103 11 0.14788635262650018 1103 12 0.30344846816119997 1103 13 0.2221628812603953 1103 14 0.2910407721294277 1103 15 0.20803076116787622 1103 16 0.38948564957554765 1103 17 0.26926558497870445 1103 18 0.4668063924946182 1103 19 0.4051569067700598 1103 20 0.4007483343251934 1103 21 0.4863932656927119 1103 22 0.45893401039713666 1103 23 0.4793027073938916 1103 24 0.6836907025324237 1103 25 0.6395668408948012 1104 1 0.09873885687418138 1104 2 0.01728124513323337 1104 3 0.04188192448599892 1104 4 0.12540365417557808 1104 5 0.10550010711379798 1104 6 0.07117406873756238 1104 7 -0.11215501695642871 1104 8 -0.03965489062563421 1104 9 -0.02130664904371571 1104 10 0.060573423437858764 1104 11 0.18894267954695465 1104 12 0.28845704977317155 1104 13 0.14131804914890012 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25 0.3316827966333804 1106 1 7.628313218361882E-4 1106 2 0.019073072868835084 1106 3 0.01499011678204809 1106 4 0.019268589001406156 1106 5 0.08756899394405337 1106 6 0.011479496277118528 1106 7 0.0726125024707045 1106 8 0.015205766243894914 1106 9 -0.07907027429240543 1106 10 -0.11400349712076974 1106 11 -0.20104771952367884 1106 12 -0.1853824597690945 1106 13 -0.2781012404860519 1106 14 -0.3903871837409767 1106 15 -0.37553946610243827 1106 16 -0.3778345412201958 1106 17 -0.18335639091888822 1106 18 -0.22479126470557986 1106 19 -0.41098072911982797 1106 20 -0.49832483845544573 1106 21 -0.4388086595369529 1106 22 -0.3337366340376846 1106 23 -0.31914145391913706 1106 24 -0.3763232278124491 1106 25 -0.3605649255647876 1107 1 -0.008339555209044391 1107 2 0.031147570669702418 1107 3 0.05573080586453827 1107 4 0.05249170207937226 1107 5 0.05944539416513095 1107 6 0.13279122524487974 1107 7 0.07657926105122925 1107 8 0.15955478221927422 1107 9 0.20003147213741101 1107 10 0.15976399077646164 1107 11 0.12878739094107772 1107 12 0.20433360684226898 1107 13 0.08812523424327878 1107 14 0.21079037805181144 1107 15 0.18172977808765012 1107 16 0.2300317030627923 1107 17 0.15763030120569965 1107 18 0.3032743025646809 1107 19 0.216228397874481 1107 20 0.13439737686209696 1107 21 0.06094501363441651 1107 22 0.11579438608166726 1107 23 0.07864200312970837 1107 24 0.1341145290841439 1107 25 0.21899291155116174 1108 1 -0.10349373182955901 1108 2 -0.1593857800398233 1108 3 -0.2264446267731266 1108 4 -0.10253337004014895 1108 5 -0.08174494461158588 1108 6 -0.12318619236540229 1108 7 -0.14391462542017308 1108 8 -0.14769776483834818 1108 9 -0.21070923950846776 1108 10 -0.14589541073480916 1108 11 -0.33441133290779135 1108 12 -0.19927824959345486 1108 13 -0.19724829561054946 1108 14 -0.18732595494807214 1108 15 -0.10606446115981072 1108 16 -0.1920176855843465 1108 17 -0.0033532306143015944 1108 18 -0.2706036502716742 1108 19 -0.11318378886025471 1108 20 -0.09372213016867192 1108 21 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-0.8255687172812074 2314 19 -0.8228629571592947 2314 20 -0.930856161084165 2314 21 -0.8667362416568025 2314 22 -0.8613247676701761 2314 23 -0.697293611298818 2314 24 -0.7608980206424718 2314 25 -0.842082391042517 2315 1 -0.44583083147688285 2315 2 -0.5368233175789037 2315 3 -0.5764419059920058 2315 4 -0.6156494857914225 2315 5 -0.5775195201953949 2315 6 -0.55389200405655 2315 7 -0.5484431345861296 2315 8 -0.6528045169957224 2315 9 -0.7774219712240509 2315 10 -0.7469973784242541 2315 11 -0.5918764889420274 2315 12 -0.7192017597091152 2315 13 -0.7863117822145469 2315 14 -1.1048423438041652 2315 15 -1.0341664339828043 2315 16 -1.0356613761955984 2315 17 -1.0195822279807913 2315 18 -1.0048472987878228 2315 19 -0.9590206343968859 2315 20 -0.9017548000763185 2315 21 -1.0152029367395272 2315 22 -1.0101976452979997 2315 23 -1.095621105663092 2315 24 -0.9421442397485507 2315 25 -1.138564649618047 2316 1 -0.9683212586844512 2316 2 -0.9518910833149157 2316 3 -1.1084760431635665 2316 4 -0.9438841710039512 2316 5 -1.008344696023343 2316 6 -1.3118617878649204 2316 7 -1.315021956365264 2316 8 -1.305058229043432 2316 9 -1.4953403117757205 2316 10 -1.7463073755787377 2316 11 -1.7785378977613178 2316 12 -1.7082982090937477 2316 13 -1.7881562111742326 2316 14 -2.046818920990333 2316 15 -1.9068997602957154 2316 16 -2.1732757623829926 2316 17 -2.006087424959617 2316 18 -1.9045160095920224 2316 19 -1.6811390390285723 2316 20 -1.883242755946256 2316 21 -1.8737835420043965 2316 22 -1.8599983923006889 2316 23 -1.9009430629266504 2316 24 -1.89814439210663 2316 25 -1.9835129711025559 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 36388.0 3 1.0 4 634.0 5 6005.0 6 7658.0 7 6163.0 8 5041.0 9 4548.0 10 4390.0 11 4237.0 12 4147.0 13 4020.0 14 3939.0 15 4046.0 16 4123.0 17 4315.0 18 4753.0 19 5255.0 20 6348.0 21 8454.0 22 10676.0 23 13541.0 24 16914.0 25 20206.0 26 23717.0 27 27571.0 28 31704.0 29 39228.0 30 50410.0 31 65697.0 32 85638.0 33 111553.0 34 146664.0 35 195682.0 36 282029.0 37 491265.0 38 1143369.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.65524419167077 19.020576008269042 13.219552630578649 25.104627169481542 2 15.359668982258622 20.83866113905738 39.60811421195287 24.193555666731125 3 18.404911383387105 25.066546217463355 30.162526572485298 26.366015826664245 4 12.836311407481576 17.470157054975317 37.85192594318219 31.84160559436092 5 13.520208681950047 37.690554459937495 35.47989171271563 13.309345145396836 6 31.625866350684245 37.28587949501602 18.156536978935392 12.931717175364344 7 27.94173860000021 31.88719066467754 22.35671688893873 17.814353846383522 8 25.685042918581598 35.928840182749866 20.531861964758953 17.854254933909587 9 26.70236629218398 15.251764642164142 19.724170398589884 38.321698667061995 10 14.954090313988436 28.783239692410138 34.28340998545652 21.9792600081449 11 34.58327156376928 22.527739018702377 24.213726973550592 18.675262443977754 12 23.129441115928078 24.681173574268772 31.394156709181487 20.795228600621666 13 28.753520865151167 21.620307957875646 25.93082248590352 23.695348691069665 14 21.64797840802214 21.171991116292617 27.595423994967245 29.584606480718 15 23.486275352572573 29.238465466965753 23.725345264377783 23.54991391608389 16 23.037403018891244 27.843624808138234 26.425661790719047 22.69331038225147 17 22.378450517284655 27.697912287103314 27.166271630775512 22.75736556483652 18 22.201595720488875 27.475784884296207 28.304474940189124 22.018144455025798 19 23.616822274160825 26.864199999722256 27.284118036720866 22.234859689396057 20 23.694793198971368 26.8620702704448 27.532549233091082 21.91058729749275 21 23.785616157043172 26.40562935692416 26.99198598493436 22.816768501098313 22 23.5066549689289 26.787946793578094 27.2341805397925 22.471217697700503 23 23.366219622827806 26.585643515029012 27.175090067836 22.87304679430718 24 23.118485837800378 26.605644742127797 27.540868289929023 22.735001130142802 25 23.31231004569666 26.62300914697021 27.38553441345305 22.679146393880078 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 469.0 1 469.0 2 565.0 3 661.0 4 661.0 5 661.0 6 1651.5 7 2642.0 8 2642.0 9 2642.0 10 3954.0 11 5266.0 12 5266.0 13 5266.0 14 9050.5 15 12835.0 16 12835.0 17 12835.0 18 22919.0 19 33003.0 20 33003.0 21 33003.0 22 50858.0 23 68713.0 24 68713.0 25 68713.0 26 96250.0 27 123787.0 28 123787.0 29 123787.0 30 157973.0 31 192159.0 32 192159.0 33 192159.0 34 221577.5 35 250996.0 36 250996.0 37 250996.0 38 279286.0 39 307576.0 40 307576.0 41 307576.0 42 330023.5 43 352471.0 44 352471.0 45 352471.0 46 363344.5 47 374218.0 48 374218.0 49 374218.0 50 369564.0 51 364910.0 52 364910.0 53 364910.0 54 334033.5 55 303157.0 56 303157.0 57 303157.0 58 261581.5 59 220006.0 60 220006.0 61 220006.0 62 179673.5 63 139341.0 64 139341.0 65 139341.0 66 107417.0 67 75493.0 68 75493.0 69 75493.0 70 54544.0 71 33595.0 72 33595.0 73 33595.0 74 23176.0 75 12757.0 76 12757.0 77 12757.0 78 8623.5 79 4490.0 80 4490.0 81 4490.0 82 2933.5 83 1377.0 84 1377.0 85 1377.0 86 852.5 87 328.0 88 328.0 89 328.0 90 195.5 91 63.0 92 63.0 93 63.0 94 39.0 95 15.0 96 15.0 97 15.0 98 8.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4432132579299101 2 0.0 3 0.0 4 0.0 5 0.058673852882778314 6 0.0 7 0.0 8 0.003749571663514828 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 1.7359128071827904E-4 20 0.0 21 0.0 22 0.0 23 0.0 24 0.005624357495272242 25 0.0010762659404533303 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2880329.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.9014739609474 #Duplication Level Percentage of deduplicated Percentage of total 1 82.05653998252234 58.17929632896247 2 11.9073830954632 16.885020249720185 3 3.0768273370101897 6.544547799520774 4 1.1551688513941143 3.2761269695046926 5 0.558803995811562 1.9810013479153412 6 0.31655122041498884 1.346636886693567 7 0.20219734275621373 1.0035262742681712 8 0.14647265972963924 0.830810197584938 9 0.10685039984551728 0.6818265758127386 >10 0.44328371051589366 5.333866885463174 >50 0.017816813782011304 0.8639365077811526 >100 0.010293226376447761 1.3469791199485923 >500 0.0010770274681581875 0.5682089129807935 >1k 6.853811157100189E-4 0.9171523580727947 >5k 4.895579397928706E-5 0.24106358577056197 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6945 0.2411182889176896 No Hit TATCAACGCAGAGTACTTTTTTTTT 4387 0.15230898970221807 No Hit GGTATCAACGCAGAGTACTTTTTTT 4227 0.1467540687192331 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.4718256143655814E-5 2 0.0 0.0 0.0 0.0 3.4718256143655814E-5 3 0.0 0.0 0.0 0.0 3.4718256143655814E-5 4 0.0 0.0 0.0 0.0 3.4718256143655814E-5 5 0.0 3.4718256143655814E-5 0.0 0.0 3.4718256143655814E-5 6 0.0 3.4718256143655814E-5 0.0 0.0 1.0415476843096744E-4 7 0.0 3.4718256143655814E-5 0.0 0.0 1.0415476843096744E-4 8 0.0 3.4718256143655814E-5 0.0 0.0 1.0415476843096744E-4 9 0.0 3.4718256143655814E-5 0.0 0.0 1.0415476843096744E-4 10 0.0 3.4718256143655814E-5 0.0 0.0 1.0415476843096744E-4 11 0.0 3.4718256143655814E-5 0.0 0.0 1.0415476843096744E-4 12 0.0 1.0415476843096744E-4 0.0 0.0 1.0415476843096744E-4 13 0.0 1.0415476843096744E-4 0.0 0.0 1.3887302457462326E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTTAA 40 0.0051420513 14.307198 1 GGTATCA 1555 0.0 12.206354 1 TAGGACC 400 0.0 11.165449 4 GTATCAA 3555 0.0 10.8393955 1 GTCCTAC 930 0.0 10.461177 1 TGGATCG 75 0.002642946 10.13601 5 GGATCGT 75 0.002656305 10.129852 6 GGACGTG 1060 0.0 10.034287 6 GCGTGCA 105 4.1246232E-5 9.948616 9 CCAACGA 115 1.0308906E-5 9.909982 19 TCCAACG 155 4.0867235E-8 9.803423 18 TAGACAG 505 0.0 9.784762 5 AGGACGT 1120 0.0 9.757041 5 TAAGACA 420 0.0 9.728761 4 TCTAAAC 355 0.0 9.636349 3 GTATTAT 320 0.0 9.538132 1 TGGACCG 150 2.6402267E-7 9.50251 5 CGTAGAG 90 0.0011114323 9.50251 2 TAGGACA 480 0.0 9.50251 4 ACCGTAT 80 0.0045302454 9.496737 8 >>END_MODULE