FastQCFastQC Report
Thu 26 May 2016
SRR1512122_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512122_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2521532
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT54630.21665400240805985No Hit
GGTATCAACGCAGAGTACTTTTTTT35570.1410650350659837No Hit
TATCAACGCAGAGTACTTTTTTTTT33910.1344817357067053No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACACG350.00224075616.20844717
GGTATCA14400.013.1328391
CGTCTCG550.003180625412.03354412
TAGGACC5800.011.7371514
GTATTAA3400.011.3572571
TAGGACT3250.011.3459144
ATATCGA600.00608910211.0307496
GGCGAGG7900.010.77143719
ATAGGAC2650.010.70369153
GTAGGAC16400.010.4926633
TTAGGAC11200.010.3835363
GGACGTG17350.010.0816236
TGTAGGA16700.010.0790632
TGGCGAG16450.010.00095718
AGGACGT17550.09.9667325
TAGGACG18500.09.863794
AAGTCGT1554.3764885E-89.7599267
GTCTAGA2750.09.7211771
TAGGACA6150.09.68553454
TATGGCG17150.09.59275516
Produced by FastQC (version 0.11.5)