##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512122_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2521532 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.522224584102045 31.0 28.0 33.0 16.0 34.0 2 25.984377354719275 31.0 25.0 33.0 10.0 34.0 3 27.649846601193243 31.0 26.0 34.0 16.0 34.0 4 31.890220310509644 35.0 32.0 37.0 19.0 37.0 5 32.28276698451576 35.0 33.0 37.0 22.0 37.0 6 32.52629353900724 35.0 33.0 37.0 24.0 37.0 7 32.55826814809409 36.0 33.0 37.0 24.0 37.0 8 32.59771956096532 36.0 33.0 37.0 25.0 37.0 9 34.095465375811216 38.0 35.0 39.0 25.0 39.0 10 33.94338164258871 38.0 34.0 39.0 25.0 39.0 11 33.95253917063119 38.0 34.0 39.0 25.0 39.0 12 33.840610390825894 38.0 34.0 39.0 23.0 39.0 13 33.82051348148665 38.0 34.0 39.0 23.0 39.0 14 34.836243601112336 39.0 34.0 41.0 23.0 41.0 15 34.75599199216984 39.0 34.0 41.0 22.0 41.0 16 34.71211112926586 39.0 34.0 41.0 22.0 41.0 17 34.644667606835846 39.0 34.0 41.0 22.0 41.0 18 34.58588588207486 39.0 34.0 41.0 19.0 41.0 19 34.60025056195995 39.0 34.0 41.0 19.0 41.0 20 34.52158925605544 39.0 34.0 41.0 18.0 41.0 21 34.4471043794011 39.0 34.0 41.0 18.0 41.0 22 34.31708857948263 39.0 34.0 41.0 18.0 41.0 23 34.19772186115425 39.0 34.0 41.0 17.0 41.0 24 34.05963438100329 39.0 34.0 41.0 10.0 41.0 25 33.92099445892418 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9384600751494467 1101 2 3.2047326742411926 1101 3 1.7636779851149207 1101 4 1.1410207042933145 1101 5 0.8666053646670449 1101 6 0.3778864429521249 1101 7 0.19359510291791082 1101 8 0.19433117220182794 1101 9 0.1676801969113697 1101 10 0.0013113331166110243 1101 11 0.15522510284522184 1101 12 0.004932716824583849 1101 13 0.09898256032050057 1101 14 0.09421378139212067 1101 15 0.2798770230680816 1101 16 0.32979330596836576 1101 17 0.17468284848071391 1101 18 0.047970723867528875 1101 19 0.03357792457722297 1101 20 0.03425172569543378 1101 21 0.3930235152588324 1101 22 0.24537656267152386 1101 23 0.23143176226381712 1101 24 0.026808593318058627 1101 25 0.07034565501091805 1102 1 1.4649094693570284 1102 2 3.8624872916935438 1102 3 2.569416973473558 1102 4 1.7499880412203517 1102 5 1.2992348111503915 1102 6 1.1596598582267958 1102 7 0.9226017601361534 1102 8 0.9786038809077837 1102 9 0.9168111689642515 1102 10 0.9401204192808592 1102 11 1.003936878509137 1102 12 0.9474768180588597 1102 13 0.9742324380892669 1102 14 1.0483734597331562 1102 15 0.9926264108804403 1102 16 1.2498705849877112 1102 17 0.8974139377105459 1102 18 0.8907988966705105 1102 19 0.9524597099577434 1102 20 1.043239073473714 1102 21 0.9625952624124423 1102 22 1.021692379289611 1102 23 0.962258994350492 1102 24 0.7737507495801168 1102 25 0.70560250563301 1103 1 1.100219192479635 1103 2 2.8481673799587774 1103 3 1.8889262652848586 1103 4 1.1753552893204677 1103 5 0.9308336573573612 1103 6 0.6913523669402366 1103 7 0.622523321567769 1103 8 0.3528981774425404 1103 9 0.4783133582875365 1103 10 0.48453146964185123 1103 11 0.5324681516034033 1103 12 0.5306685822108221 1103 13 0.4807005953575896 1103 14 0.5234176276374001 1103 15 0.4501640582006061 1103 16 0.3250099613170718 1103 17 0.4562791594809781 1103 18 0.2928089942078955 1103 19 0.5323127939142367 1103 20 0.6185910136184347 1103 21 0.6352898355994299 1103 22 0.5198737404172178 1103 23 0.5010761716576155 1103 24 0.405052342514864 1103 25 0.3619360503539184 1104 1 1.3236390758742473 1104 2 3.7623662535194065 1104 3 2.317820646655992 1104 4 1.727922129773905 1104 5 1.2549000500014742 1104 6 0.8966410067123718 1104 7 0.9496179154708884 1104 8 0.9091083999078791 1104 9 0.995511349434345 1104 10 1.1027826792882536 1104 11 0.9784862468638167 1104 12 1.020567205308545 1104 13 0.9523042347451351 1104 14 1.1138572862789289 1104 15 0.8802493516007246 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2316 3 -1.190492040908616 2316 4 -1.718872831219052 2316 5 -1.6800149971252587 2316 6 -1.72312044602468 2316 7 -1.6516391172856402 2316 8 -1.8588643482105454 2316 9 -2.052331387653453 2316 10 -2.2162297678844354 2316 11 -2.4293096700455585 2316 12 -2.277591282754763 2316 13 -2.598360668301055 2316 14 -2.780466766721382 2316 15 -2.9609909512856305 2316 16 -2.770410675850215 2316 17 -2.7064176184072934 2316 18 -2.506112835231285 2316 19 -2.8611076980430603 2316 20 -2.7774648764548893 2316 21 -2.862616448485152 2316 22 -2.8796432596205825 2316 23 -3.0070675525108435 2316 24 -2.9483580391290722 2316 25 -3.171950358997389 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 124248.0 3 9.0 4 1043.0 5 5158.0 6 5913.0 7 5396.0 8 4980.0 9 4978.0 10 4893.0 11 4652.0 12 4510.0 13 4536.0 14 4654.0 15 4726.0 16 4959.0 17 5359.0 18 6031.0 19 7608.0 20 9871.0 21 12901.0 22 15977.0 23 18983.0 24 21997.0 25 24922.0 26 27808.0 27 31475.0 28 36475.0 29 43823.0 30 53910.0 31 67645.0 32 85305.0 33 111322.0 34 145172.0 35 194940.0 36 275401.0 37 378025.0 38 761927.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.7987033279768 19.077211790292566 12.869358786642406 25.254726095088227 2 16.284975864250057 21.78949672299518 38.904179224607624 23.02134818814713 3 18.29368019124881 25.043108713274307 30.11986363845472 26.543347457022158 4 12.516359102323507 17.37634104980623 38.36270965428953 31.74459019358073 5 13.434372437073968 37.66008125219113 35.57107345851649 13.334472852218413 6 31.065677532547674 37.204445551355285 18.62514534814549 13.104731567951546 7 27.56320363969206 31.97191231362521 22.78583020163932 17.67905384504341 8 25.067697154577946 36.11502408860673 21.18682986955508 17.630448887260243 9 26.43190726907293 15.613008282266494 20.54326496748802 37.41181948117256 10 15.37430419284784 28.596979931248146 34.288559494783335 21.740156381120684 11 33.49455807025253 22.875815179026084 24.9012901680407 18.728336582680686 12 23.09357961747065 24.9012901680407 31.617048683102176 20.388081531386472 13 28.50838299890701 22.141658325176916 26.042659779848126 23.307298896067945 14 21.37933523403948 21.956606315478883 27.821563113345565 28.842495337136064 15 23.059513026207874 30.056608442803817 23.84280667467238 23.041071856315924 16 22.512900887238395 28.017411637052398 27.220634122430333 22.24905335327888 17 21.84652029004589 28.385362549434234 27.681980637168195 22.086136523351676 18 21.75126074148573 27.46663536294602 29.24222258531718 21.53988131025107 19 23.374825353981652 27.109662431009124 27.66448452506396 21.85102768994527 20 23.483081276972754 27.246635569095858 27.999037091031383 21.271246062900005 21 23.168939822671224 26.74843180591474 27.375947390692403 22.706680980721632 22 23.292745364996257 27.48073680983566 27.405583697763134 21.820934127404946 23 22.644961231702002 27.385952673765296 27.707822150061133 22.261263944471565 24 22.999555428119546 26.77528338978722 28.145921102569993 22.07924007952324 25 22.913966588565998 27.097534356097803 27.82264908793543 22.16584996740077 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1084.0 1 1084.0 2 998.5 3 913.0 4 913.0 5 913.0 6 1972.0 7 3031.0 8 3031.0 9 3031.0 10 4338.0 11 5645.0 12 5645.0 13 5645.0 14 9261.0 15 12877.0 16 12877.0 17 12877.0 18 22597.0 19 32317.0 20 32317.0 21 32317.0 22 49473.5 23 66630.0 24 66630.0 25 66630.0 26 92474.5 27 118319.0 28 118319.0 29 118319.0 30 150735.0 31 183151.0 32 183151.0 33 183151.0 34 209062.0 35 234973.0 36 234973.0 37 234973.0 38 256222.5 39 277472.0 40 277472.0 41 277472.0 42 294393.0 43 311314.0 44 311314.0 45 311314.0 46 318034.5 47 324755.0 48 324755.0 49 324755.0 50 315792.0 51 306829.0 52 306829.0 53 306829.0 54 277066.5 55 247304.0 56 247304.0 57 247304.0 58 212418.5 59 177533.0 60 177533.0 61 177533.0 62 144949.5 63 112366.0 64 112366.0 65 112366.0 66 86774.0 67 61182.0 68 61182.0 69 61182.0 70 44280.5 71 27379.0 72 27379.0 73 27379.0 74 19167.0 75 10955.0 76 10955.0 77 10955.0 78 7423.5 79 3892.0 80 3892.0 81 3892.0 82 2522.0 83 1152.0 84 1152.0 85 1152.0 86 742.5 87 333.0 88 333.0 89 333.0 90 210.0 91 87.0 92 87.0 93 87.0 94 59.5 95 32.0 96 32.0 97 32.0 98 19.5 99 7.0 100 7.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.508885867797831 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 3.17267439001369E-4 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 5.155595883772246E-4 15 0.0 16 0.0 17 0.0 18 0.0 19 1.1897528962551338E-4 20 2.3795057925102677E-4 21 3.965842987517112E-5 22 5.155595883772246E-4 23 5.155595883772246E-4 24 1.9829214937585563E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2521532.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.37436342193598 #Duplication Level Percentage of deduplicated Percentage of total 1 82.38254079680566 59.623839472504805 2 11.950137174917467 17.297671416789285 3 3.036794628142187 6.593582341648367 4 1.1197481721509128 3.2416424460919484 5 0.5066741698294731 1.8335110251873 6 0.2739158848670759 1.1894692679046555 7 0.16597156581193315 0.840846049524643 8 0.11315800774424332 0.6551791021267289 9 0.07780662814566632 0.5068084663844897 >10 0.3440543484865527 4.156839453521355 >50 0.017008232530580706 0.8318676913524158 >100 0.01031142788140327 1.4575300768678978 >500 0.0011052721687322216 0.5594336304341612 >1k 7.184269096603921E-4 0.9932776705497547 >5k 5.526360843541478E-5 0.21850188911223764 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5463 0.21665400240805985 No Hit GGTATCAACGCAGAGTACTTTTTTT 3557 0.1410650350659837 No Hit TATCAACGCAGAGTACTTTTTTTTT 3391 0.1344817357067053 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.1897528962551338E-4 0.0 0.0 0.0 6 0.0 1.1897528962551338E-4 0.0 0.0 0.0 7 0.0 1.1897528962551338E-4 0.0 0.0 0.0 8 0.0 1.1897528962551338E-4 0.0 0.0 0.0 9 0.0 1.1897528962551338E-4 0.0 0.0 0.0 10 0.0 1.1897528962551338E-4 0.0 1.1897528962551338E-4 0.0 11 0.0 1.1897528962551338E-4 0.0 1.586337195006845E-4 0.0 12 0.0 1.1897528962551338E-4 0.0 1.586337195006845E-4 0.0 13 0.0 1.1897528962551338E-4 0.0 1.586337195006845E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGACACG 35 0.002240756 16.208447 17 GGTATCA 1440 0.0 13.132839 1 CGTCTCG 55 0.0031806254 12.033544 12 TAGGACC 580 0.0 11.737151 4 GTATTAA 340 0.0 11.357257 1 TAGGACT 325 0.0 11.345914 4 ATATCGA 60 0.006089102 11.030749 6 GGCGAGG 790 0.0 10.771437 19 ATAGGAC 265 0.0 10.7036915 3 GTAGGAC 1640 0.0 10.492663 3 TTAGGAC 1120 0.0 10.383536 3 GGACGTG 1735 0.0 10.081623 6 TGTAGGA 1670 0.0 10.079063 2 TGGCGAG 1645 0.0 10.000957 18 AGGACGT 1755 0.0 9.966732 5 TAGGACG 1850 0.0 9.86379 4 AAGTCGT 155 4.3764885E-8 9.759926 7 GTCTAGA 275 0.0 9.721177 1 TAGGACA 615 0.0 9.6855345 4 TATGGCG 1715 0.0 9.592755 16 >>END_MODULE