FastQCFastQC Report
Thu 26 May 2016
SRR1512122_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512122_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2521532
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT68790.27281033911130215No Hit
GGTATCAACGCAGAGTACTTTTTTT46750.18540315966642504No Hit
TATCAACGCAGAGTACTTTTTTTTT45720.1813183413892824No Hit
GTCCTACAGTGGACATTTCTAAATT26910.1067208347940855No Hit
CTTTAGGACGTGAAATATGGCGAGG26730.10600698305633241No Hit
GTCCTAAAGTGTGTATTTCTCATTT25690.10188250634931462No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15750.012.8981991
TAGGACC5850.011.0459534
GTATAAG3400.010.3789361
ATAGGAC2750.010.3666553
ACCGTGC1106.0737184E-610.3600788
GGCGAGG7300.010.01770319
GTCCTAC15600.09.7819491
GTATCAA31800.09.7773361
GTCTTAA2350.09.7403241
TAAGCCC1002.7483102E-49.50276854
GTCCTAA9550.09.4874671
AGGACGT14900.09.4389915
CTAAGAC3850.09.3793553
GGACGTG14900.09.3055296
TTAGGAC10050.09.2663813
TGGCGAG15800.09.25649718
AAGGCGT2002.6739144E-99.0219026
CCCTATA1401.0815147E-58.8239992
TCCTACA18100.08.820252
AACTATC1304.387853E-58.7662197
Produced by FastQC (version 0.11.5)