FastQCFastQC Report
Thu 26 May 2016
SRR1512121_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512121_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2172283
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT38030.17506927044036158No Hit
TATCAACGCAGAGTACTTTTTTTTT23110.10638577017819502No Hit
GGTATCAACGCAGAGTACTTTTTTT22910.10546507982615524No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA11050.012.8156591
TATTCCG604.2617787E-412.6061935
TAATACG550.003181096412.0331834
TAGGACC4850.011.8914074
CGTTAGG853.485103E-511.6505991
TAGGACT3100.010.9795874
TAGACTC1900.010.9474825
GTAGGAC12750.010.0107983
TTAGGAC9600.09.9470743
GGTTTAC1201.0390993E-59.9030091
GTCTTAA1802.042725E-99.9030091
AGGACGT12800.09.897835
ACCGTGC1151.0865137E-59.8659428
GTCCTAC11750.09.77658751
TGTAGGA13050.09.7132972
GGACGTG12950.09.56415756
GTATCAA20700.09.5202841
GTATAAT2501.8189894E-129.5068881
GTTCTAG2403.6379788E-129.4903841
TAGGACG13950.09.4885324
Produced by FastQC (version 0.11.5)