##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512121_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2172283 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.3618414359455 31.0 28.0 33.0 16.0 34.0 2 25.848567612967557 31.0 25.0 33.0 10.0 34.0 3 27.496962872701207 31.0 26.0 34.0 16.0 34.0 4 31.724896802120167 35.0 32.0 37.0 19.0 37.0 5 32.09947967184755 35.0 33.0 37.0 19.0 37.0 6 32.342065007183685 35.0 33.0 37.0 22.0 37.0 7 32.371860388356396 35.0 33.0 37.0 22.0 37.0 8 32.401014508698914 36.0 33.0 37.0 23.0 37.0 9 33.883319990995645 38.0 34.0 39.0 23.0 39.0 10 33.72642560844973 38.0 34.0 39.0 23.0 39.0 11 33.73394580724519 38.0 34.0 39.0 23.0 39.0 12 33.620012677906146 38.0 34.0 39.0 22.0 39.0 13 33.599161803503506 38.0 34.0 39.0 22.0 39.0 14 34.602909933926654 39.0 34.0 41.0 20.0 41.0 15 34.520390299054036 39.0 34.0 41.0 19.0 41.0 16 34.479438452540485 39.0 34.0 41.0 19.0 41.0 17 34.39859999825069 39.0 34.0 41.0 18.0 41.0 18 34.340007724592056 39.0 34.0 41.0 18.0 41.0 19 34.3407171165083 39.0 34.0 41.0 18.0 41.0 20 34.25684406681818 39.0 34.0 41.0 18.0 41.0 21 34.17517561017602 39.0 34.0 41.0 17.0 41.0 22 34.05568427318172 39.0 34.0 41.0 13.0 41.0 23 33.925225672713914 39.0 34.0 41.0 10.0 41.0 24 33.788752202176234 39.0 34.0 41.0 2.0 41.0 25 33.644577156843745 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7168537251459242 1101 2 2.95421337892914 1101 3 1.57045161839698 1101 4 0.9212119241057799 1101 5 0.7579719390756523 1101 6 0.3102772860513383 1101 7 0.1590392871227806 1101 8 0.24216788798054267 1101 9 0.010606025804641206 1101 10 -0.124063617777324 1101 11 0.08929961173634382 1101 12 0.07085171349432073 1101 13 0.13538252885555124 1101 14 0.07391479375579735 1101 15 0.15528427037801862 1101 16 0.06741149184588124 1101 17 -0.07068208116796626 1101 18 -0.17554723233865133 1101 19 -0.06160794056302876 1101 20 -0.03639735421347012 1101 21 0.22118122950687535 1101 22 -0.11117171688022864 1101 23 0.19161104420301456 1101 24 0.03833292576531022 1101 25 -0.05662907957359664 1102 1 1.2895897232753626 1102 2 3.8357768441446147 1102 3 2.3967260328093545 1102 4 1.4707789795928257 1102 5 1.1482593493660005 1102 6 1.0203854844666438 1102 7 0.8525952776342081 1102 8 0.7184473936393729 1102 9 0.8761678919915994 1102 10 0.705047752295954 1102 11 0.8996421960530441 1102 12 0.7448978590436326 1102 13 0.8048710660084453 1102 14 0.8403227496769148 1102 15 0.9992460520879973 1102 16 0.9701913124077279 1102 17 0.6430673012826915 1102 18 0.622006895153703 1102 19 0.6928167297316179 1102 20 0.9720767426559789 1102 21 0.9302023525227057 1102 22 1.0078182552499086 1102 23 0.9701597372611914 1102 24 0.9031037631056478 1102 25 0.7656996188398253 1103 1 0.8733201230028804 1103 2 2.8268842989610405 1103 3 1.660337420745627 1103 4 1.014293792091589 1103 5 0.7733262529509517 1103 6 0.6636096155309872 1103 7 0.6263048268707436 1103 8 0.33145137811954584 1103 9 0.4863439047361666 1103 10 0.3802004366389866 1103 11 0.4008442079362027 1103 12 0.356960237513384 1103 13 0.37474679203624106 1103 14 0.28241938638639397 1103 15 0.24592249824165435 1103 16 0.15749341746032286 1103 17 0.2863026955768291 1103 18 0.25973952590177873 1103 19 0.24615082596105253 1103 20 0.3698998757080574 1103 21 0.42822047460890644 1103 22 0.22597440145924708 1103 23 0.41308579821238567 1103 24 0.23222029338210604 1103 25 0.13176404811845543 1104 1 1.4672047430979838 1104 2 3.9644987620958787 1104 3 2.5079982814977484 1104 4 1.7832841557999153 1104 5 1.5007788569151614 1104 6 0.9898062533899505 1104 7 1.0377486554019058 1104 8 1.008676875154464 1104 9 1.0203203083350871 1104 10 0.9897553461068327 1104 11 0.8983179872475517 1104 12 0.8997861789998467 1104 13 0.7631391341219356 1104 14 0.9901050789160948 1104 15 0.7860997463695085 1104 16 1.0711417540507213 1104 17 1.0789156411155432 1104 18 1.130114185773877 1104 19 1.2342416900323627 1104 20 0.9558774649844253 1104 21 1.0033885195368555 1104 22 1.0377880442046958 1104 23 1.0916043581467818 1104 24 1.0861911590863897 1104 25 0.9417077639392559 1105 1 1.4035242267586696 1105 2 3.849299921779263 1105 3 2.3236840908479337 1105 4 1.8698738339989553 1105 5 1.3592559258351855 1105 6 1.009359149547059 1105 7 0.6038405946053373 1105 8 0.752140548235829 1105 9 0.8168883606566482 1105 10 0.7948351495485753 1105 11 0.6452000273688796 1105 12 0.6115012417329382 1105 13 0.7910471313129719 1105 14 0.6568110692669649 1105 15 0.608135363742754 1105 16 0.5241400667853213 1105 17 0.8589140251914742 1105 18 0.5980847226646517 1105 19 0.8031213131438619 1105 20 0.8383005087493203 1105 21 0.7804176431301144 1105 22 0.8305294507978331 1105 23 0.9523295384555439 1105 24 0.89962198077518 1105 25 0.9309678855977097 1106 1 0.6834263481085543 1106 2 2.8836119665212117 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-2.1411576429022716 2315 18 -2.3618364675789323 2315 19 -2.200249601931965 2315 20 -2.2073784709987834 2315 21 -2.0992780315118225 2315 22 -2.3018842677326425 2315 23 -2.1123641181637574 2315 24 -2.10534449238763 2315 25 -2.0409495376702758 2316 1 -1.4563873148180306 2316 2 -2.004997606756884 2316 3 -1.1960674922685293 2316 4 -1.7910866970139736 2316 5 -1.622462194849696 2316 6 -1.7412039434526996 2316 7 -1.6230724197823605 2316 8 -1.8136184258646537 2316 9 -1.9106700874300486 2316 10 -2.035723227417847 2316 11 -2.322471149795849 2316 12 -2.1233119916085172 2316 13 -2.30462827301956 2316 14 -2.5139228021406836 2316 15 -2.729183409039038 2316 16 -2.5097734261105913 2316 17 -2.4182801559276825 2316 18 -2.3486651846373334 2316 19 -2.6702622716076796 2316 20 -2.70238442777066 2316 21 -2.9116214094926676 2316 22 -2.519011132982186 2316 23 -2.6129115696120486 2316 24 -2.553443475419911 2316 25 -2.9863758621366223 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 114726.0 3 0.0 4 897.0 5 4701.0 6 5532.0 7 4970.0 8 4489.0 9 4424.0 10 4290.0 11 4078.0 12 4054.0 13 4010.0 14 4137.0 15 4289.0 16 4661.0 17 4892.0 18 5504.0 19 6991.0 20 9073.0 21 11510.0 22 14206.0 23 16883.0 24 19745.0 25 22213.0 26 24839.0 27 28056.0 28 32147.0 29 39058.0 30 47811.0 31 59360.0 32 75175.0 33 95955.0 34 125285.0 35 167604.0 36 234356.0 37 318900.0 38 643462.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.23386962011856 18.61898288574739 12.787422264962714 25.35972522917134 2 16.640548226855284 21.56063544476408 38.29130434363316 23.507511984747474 3 18.56756232958597 24.58583895376431 29.68255057006845 27.164048146581273 4 13.029701931101979 17.035901859932615 37.317375314358216 32.617020894607194 5 13.999419044387862 37.1829545229604 35.01647805557563 13.8011483770761 6 31.950763321353616 36.20366223001331 18.339783536491332 13.505790912141741 7 28.23623809604918 31.474628305796255 22.245213906291212 18.04391969186335 8 25.79424796584226 35.418489832088476 20.70161466665132 18.085647535417937 9 26.712955908599383 15.695791018021133 20.0755150226743 37.515738050705174 10 16.022313851372036 28.210504800709668 33.444675486573345 22.322505861344954 11 34.09841167103918 22.64332041451321 24.31036840043401 18.947899514013596 12 23.729090546673707 24.39479570571606 30.7880695102802 21.088044237330035 13 28.83348992741738 21.75582094966448 25.702682385306154 23.708006737611996 14 22.01473848578815 21.401969919052494 27.076443465643347 29.506848129516012 15 23.592137856807792 29.447037978016677 23.42494048887737 23.535883676298162 16 23.254935015373228 27.539459637625484 26.445495361331833 22.760109985669455 17 22.53883243519948 27.80035004663299 27.04639637026868 22.614421147898845 18 22.496654441433275 27.10111896101935 28.35965663774011 22.042569959807263 19 24.05167480942808 26.64802877349436 26.949693800560425 22.350602616517136 20 24.0925203012503 26.766761191006683 27.228211832728743 21.91250667501427 21 23.85478674259914 26.25553508040626 26.68420890299183 23.205469274002766 22 23.963124306313528 26.870680499808724 26.74749145019199 22.418703743685757 23 23.408646254839407 26.7957482265096 26.984905191343618 22.81070032730738 24 23.58778369425262 26.381165937861518 27.288370682007866 22.742679685878 25 23.539070894110434 26.62527240938331 27.05670810695849 22.778948589547767 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 865.0 1 865.0 2 773.0 3 681.0 4 681.0 5 681.0 6 1321.5 7 1962.0 8 1962.0 9 1962.0 10 2799.5 11 3637.0 12 3637.0 13 3637.0 14 6111.5 15 8586.0 16 8586.0 17 8586.0 18 15371.0 19 22156.0 20 22156.0 21 22156.0 22 35024.0 23 47892.0 24 47892.0 25 47892.0 26 68464.5 27 89037.0 28 89037.0 29 89037.0 30 115132.0 31 141227.0 32 141227.0 33 141227.0 34 164348.0 35 187469.0 36 187469.0 37 187469.0 38 207900.0 39 228331.0 40 228331.0 41 228331.0 42 246349.0 43 264367.0 44 264367.0 45 264367.0 46 273042.0 47 281717.0 48 281717.0 49 281717.0 50 278245.0 51 274773.0 52 274773.0 53 274773.0 54 252393.5 55 230014.0 56 230014.0 57 230014.0 58 199867.0 59 169720.0 60 169720.0 61 169720.0 62 140313.0 63 110906.0 64 110906.0 65 110906.0 66 86601.5 67 62297.0 68 62297.0 69 62297.0 70 45644.0 71 28991.0 72 28991.0 73 28991.0 74 20364.5 75 11738.0 76 11738.0 77 11738.0 78 7893.0 79 4048.0 80 4048.0 81 4048.0 82 2702.0 83 1356.0 84 1356.0 85 1356.0 86 859.0 87 362.0 88 362.0 89 362.0 90 235.0 91 108.0 92 108.0 93 108.0 94 74.5 95 41.0 96 41.0 97 41.0 98 21.5 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.529658428482845 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 3.682761408159066E-4 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 5.063796936218716E-4 15 0.0 16 0.0 17 4.603451760198832E-5 18 0.0 19 2.7620710561192995E-4 20 1.3810355280596497E-4 21 9.206903520397664E-5 22 5.524142112238599E-4 23 5.984487288258482E-4 24 2.7620710561192995E-4 25 4.603451760198832E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2172283.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.48652731827906 #Duplication Level Percentage of deduplicated Percentage of total 1 83.13472808646078 61.092824666234016 2 11.2690414069127 16.56245438399816 3 2.9116867144529825 6.419092358517578 4 1.1012193986729737 3.2369915769600124 5 0.5393838296803809 1.9818722267422644 6 0.3050337866230111 1.344952421620201 7 0.18833302130589485 0.9687957800590773 8 0.11502287958308921 0.6762105586167847 9 0.09326959389940236 0.6168652704047903 >10 0.31933482919190725 3.8025180719026124 >50 0.01258484982903609 0.6400904471874859 >100 0.008720940669960008 1.2578321769253809 >500 0.0010096385956248686 0.5296583729233296 >1k 6.310241222648859E-4 0.8698416879082871 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3803 0.17506927044036158 No Hit TATCAACGCAGAGTACTTTTTTTTT 2311 0.10638577017819502 No Hit GGTATCAACGCAGAGTACTTTTTTT 2291 0.10546507982615524 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.3810355280596497E-4 0.0 0.0 0.0 6 0.0 1.3810355280596497E-4 0.0 0.0 4.603451760198832E-5 7 0.0 1.3810355280596497E-4 0.0 0.0 4.603451760198832E-5 8 0.0 1.3810355280596497E-4 0.0 0.0 4.603451760198832E-5 9 0.0 1.3810355280596497E-4 0.0 0.0 4.603451760198832E-5 10 0.0 1.3810355280596497E-4 0.0 0.0 4.603451760198832E-5 11 0.0 1.3810355280596497E-4 0.0 0.0 4.603451760198832E-5 12 0.0 1.3810355280596497E-4 0.0 0.0 4.603451760198832E-5 13 0.0 1.3810355280596497E-4 0.0 0.0 4.603451760198832E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1105 0.0 12.815659 1 TATTCCG 60 4.2617787E-4 12.606193 5 TAATACG 55 0.0031810964 12.033183 4 TAGGACC 485 0.0 11.891407 4 CGTTAGG 85 3.485103E-5 11.650599 1 TAGGACT 310 0.0 10.979587 4 TAGACTC 190 0.0 10.947482 5 GTAGGAC 1275 0.0 10.010798 3 TTAGGAC 960 0.0 9.947074 3 GGTTTAC 120 1.0390993E-5 9.903009 1 GTCTTAA 180 2.042725E-9 9.903009 1 AGGACGT 1280 0.0 9.89783 5 ACCGTGC 115 1.0865137E-5 9.865942 8 GTCCTAC 1175 0.0 9.7765875 1 TGTAGGA 1305 0.0 9.713297 2 GGACGTG 1295 0.0 9.5641575 6 GTATCAA 2070 0.0 9.520284 1 GTATAAT 250 1.8189894E-12 9.506888 1 GTTCTAG 240 3.6379788E-12 9.490384 1 TAGGACG 1395 0.0 9.488532 4 >>END_MODULE