FastQCFastQC Report
Thu 26 May 2016
SRR1512121_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512121_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2172283
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT47400.21820361343342465No Hit
TATCAACGCAGAGTACTTTTTTTTT30440.14012907158045246No Hit
GGTATCAACGCAGAGTACTTTTTTT29510.13584786144346755No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATCG604.0887983E-412.6682795
GGTATCA13300.012.0531551
TAGGACC4950.010.9407864
GTATAAC1553.4069672E-910.4655081
GTATAGG2700.010.2489011
GTCCTAC11950.09.98125651
GTAGGAC12050.09.7771783
GTATCAA25900.09.5789221
GTCGTAG900.00106974879.5420811
GCGCCCA800.0045282429.49705319
GTATAAG2159.822543E-119.3201721
TCAATAC1651.0637632E-79.2132933
TAACCCC1451.7036564E-69.1735814
GTCTACG1252.6147007E-59.1603981
TCCTACA14050.09.1292752
CCGTCCT1151.115146E-49.083729
TAGAACC1804.2104148E-88.9733644
TCTTATA2651.8189894E-128.9634052
AGGACGT12550.08.9334085
GTCCTAA7700.08.9224651
Produced by FastQC (version 0.11.5)