##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512121_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2172283 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.205479212423057 31.0 31.0 34.0 28.0 34.0 2 31.391901055249246 33.0 31.0 34.0 28.0 34.0 3 31.4964063153834 33.0 31.0 34.0 28.0 34.0 4 35.06696503172009 37.0 35.0 37.0 32.0 37.0 5 34.714197919884285 37.0 35.0 37.0 32.0 37.0 6 34.670021355412715 37.0 35.0 37.0 32.0 37.0 7 34.520140791968636 37.0 35.0 37.0 31.0 37.0 8 34.43714285845813 37.0 35.0 37.0 30.0 37.0 9 35.98828191354441 39.0 37.0 39.0 31.0 39.0 10 35.8253095936395 39.0 35.0 39.0 30.0 39.0 11 35.81207098706752 39.0 35.0 39.0 30.0 39.0 12 35.660360091203586 39.0 35.0 39.0 30.0 39.0 13 35.63313205507754 39.0 35.0 39.0 30.0 39.0 14 36.73050610808997 40.0 36.0 41.0 30.0 41.0 15 36.67379250309467 40.0 36.0 41.0 30.0 41.0 16 36.58882337154045 40.0 36.0 41.0 30.0 41.0 17 36.52606543438401 40.0 36.0 41.0 30.0 41.0 18 36.45775619474995 40.0 36.0 41.0 30.0 41.0 19 36.475761675619616 40.0 36.0 41.0 30.0 41.0 20 36.373905701973456 40.0 36.0 41.0 30.0 41.0 21 36.265360912919725 40.0 36.0 41.0 29.0 41.0 22 36.16800481336916 39.0 36.0 41.0 29.0 41.0 23 36.22979004116867 40.0 36.0 41.0 30.0 41.0 24 36.12615759548825 40.0 36.0 41.0 29.0 41.0 25 35.724518858730654 39.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.046467709240133104 1101 2 -0.041559073396854274 1101 3 -0.003911249398363736 1101 4 -0.05787688649392919 1101 5 0.001435883075764366 1101 6 -0.13980351291613147 1101 7 -0.07836528862738135 1101 8 -0.22174747408295303 1101 9 -0.11446367392065326 1101 10 -0.11345069938639796 1101 11 -0.11779249256039748 1101 12 -0.15636906400492023 1101 13 -0.0026743353060325603 1101 14 -0.10755251310003189 1101 15 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-0.004747418463065145 1103 2 0.09518485282361766 1103 3 0.1523171979560196 1103 4 0.1946871231207581 1103 5 0.0734642438012898 1103 6 0.08628617955143625 1103 7 0.15099761749184637 1103 8 0.10889915640780146 1103 9 0.005567947470353829 1103 10 -0.041313123098163373 1103 11 -0.024801250254284923 1103 12 0.09866042884448234 1103 13 -0.1718627196035314 1103 14 0.12481080013763801 1103 15 0.06280246828031721 1103 16 0.04655571158858862 1103 17 0.11732085179205143 1103 18 0.046912155769284425 1103 19 0.06806290126441894 1103 20 0.06740939199904261 1103 21 0.06611475010494416 1103 22 0.08079815207688767 1103 23 0.03893600219509352 1103 24 0.17086754142556515 1103 25 0.02108408516851057 1104 1 0.21820742107111002 1104 2 0.22840716201918454 1104 3 0.13285203078082475 1104 4 0.22784615477017667 1104 5 0.27185621314407626 1104 6 0.19713193321097577 1104 7 0.1338696371016752 1104 8 0.21053949145043305 1104 9 0.35819871367002776 1104 10 0.2173447184364008 1104 11 0.4607798115660131 1104 12 0.3498043945805307 1104 13 0.27541545842912996 1104 14 0.3815046415373047 1104 15 0.4505030172773701 1104 16 0.5440538966856181 1104 17 0.44908023381980655 1104 18 0.5110937809638614 1104 19 0.5072552206305403 1104 20 0.46385874955001327 1104 21 0.5177464765613493 1104 22 0.41151873511779513 1104 23 0.5381860789623829 1104 24 0.3909145425313625 1104 25 0.5387165997099217 1105 1 -0.12812271221683957 1105 2 -0.08041333374288229 1105 3 -0.10983183000559649 1105 4 0.01318439432404972 1105 5 0.11740159181611176 1105 6 0.009634028325294253 1105 7 0.10142931670175415 1105 8 -0.07435028619669737 1105 9 -0.053958622324422834 1105 10 0.10539126392595222 1105 11 -0.0111503839522058 1105 12 -0.143316969053096 1105 13 0.03724286226660922 1105 14 -0.023746338116666266 1105 15 -0.07073435079488632 1105 16 -0.15532835617040064 1105 17 0.024972150847268892 1105 18 -0.18412338461936173 1105 19 -0.2025282541391249 1105 20 -0.030027384532139934 1105 21 -0.15676537254572054 1105 22 -0.051998340816027167 1105 23 -0.1903898191862723 1105 24 -0.06162714272272751 1105 25 -0.03981258276142796 1106 1 -0.2123939141138571 1106 2 -0.10288072817662908 1106 3 -0.13222736085670306 1106 4 -0.11510043597201758 1106 5 -0.04768978306713478 1106 6 -0.16393409542244086 1106 7 -0.12677540484826721 1106 8 -0.3014835870600052 1106 9 -0.17972912833866417 1106 10 -0.27758373520672563 1106 11 -0.3258675951225527 1106 12 -0.2970106445622136 1106 13 -0.43884436164994867 1106 14 -0.4519169976801791 1106 15 -0.590626145415527 1106 16 -0.5486737884174602 1106 17 -0.4466142501298975 1106 18 -0.5484703969641274 1106 19 -0.4771174264824154 1106 20 -0.7024844387199849 1106 21 -0.6272503909035976 1106 22 -0.6125013949056495 1106 23 -0.6365545341221335 1106 24 -0.6870948755235631 1106 25 -0.650026537993341 1107 1 0.24939757103212656 1107 2 0.17531205549715523 1107 3 0.11541461546981324 1107 4 0.2146256307435337 1107 5 0.2643958019603758 1107 6 0.29599063476003806 1107 7 0.3288805672906179 1107 8 0.36469111281085276 1107 9 0.22275563097824858 1107 10 0.33241239813266077 1107 11 0.3061006056722064 1107 12 0.3997512366012401 1107 13 0.4130424761403262 1107 14 0.5311245872185992 1107 15 0.39413074656531677 1107 16 0.3967743319754504 1107 17 0.3171557131037588 1107 18 0.3751496086176189 1107 19 0.3688232632645878 1107 20 0.4135096479787421 1107 21 0.3899416992402962 1107 22 0.4518813701049922 1107 23 0.40754655987387167 1107 24 0.413261500961319 1107 25 0.5402490971508556 1108 1 -0.024930055388399097 1108 2 0.048388329627471194 1108 3 -0.012579230989420154 1108 4 0.03580095703006947 1108 5 0.03195790805933285 1108 6 0.08536545486822433 1108 7 -0.11802866798306866 1108 8 0.05582386056530453 1108 9 0.059886095967577546 1108 10 -0.04935208005700531 1108 11 -0.1839358193191103 1108 12 0.04999648497601328 1108 13 -0.005548760026613309 1108 14 -0.08899630008228598 1108 15 -0.11610338573581203 1108 16 0.04651717352177087 1108 17 -0.035104323618988076 1108 18 0.008242556858405692 1108 19 0.001417179199279417 1108 20 0.11840488890971557 1108 21 0.13856380236173038 1108 22 0.23094623040969253 1108 23 0.21718419572320613 1108 24 0.16078271578936665 1108 25 0.26503675036905605 1109 1 -0.23057381254762177 1109 2 -0.15468154437023784 1109 3 -0.05663192285654617 1109 4 -0.07066181502033686 1109 5 -0.11441895333815211 1109 6 -0.06708700790382238 1109 7 -0.07800690395099252 1109 8 -0.0676217293545065 1109 9 -0.21146900139783043 1109 10 -0.31962355772023443 1109 11 -0.23977545276699175 1109 12 -0.12201496977770887 1109 13 -0.2754101575065633 1109 14 -0.25816812138812395 1109 15 -0.3147643250256138 1109 16 -0.3625194816901427 1109 17 -0.2934766031149749 1109 18 -0.1339544649056279 1109 19 -0.3224087085441383 1109 20 -0.22725605038009178 1109 21 -0.1893079043965784 1109 22 -0.35158870658916186 1109 23 -0.1651285190534253 1109 24 -0.18346127046492455 1109 25 -0.40739509384629 1110 1 -0.15080731858641627 1110 2 -0.21517561167231847 1110 3 -0.23195449304392923 1110 4 -0.15388610224189847 1110 5 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2314 18 -0.6771938619038593 2314 19 -0.6060021181076607 2314 20 -0.7962300317252087 2314 21 -0.6942501801601182 2314 22 -0.6024498815931594 2314 23 -0.6234535784875987 2314 24 -0.49154473204369964 2314 25 -0.6781453434422531 2315 1 -0.5155704856651937 2315 2 -0.548657452872856 2315 3 -0.5881673196684183 2315 4 -0.47688328801781665 2315 5 -0.5004980803966603 2315 6 -0.5829693214665497 2315 7 -0.5424594695047773 2315 8 -0.6643346072295202 2315 9 -0.8163788915701389 2315 10 -0.5318487349172969 2315 11 -0.4681740457391541 2315 12 -0.7195058107080996 2315 13 -0.6510248193208525 2315 14 -0.7269750486064837 2315 15 -0.8601747487162612 2315 16 -0.8590926913608214 2315 17 -0.952877378846587 2315 18 -0.9483215255238591 2315 19 -0.7044991267170317 2315 20 -0.9177373123233963 2315 21 -0.8185045434338889 2315 22 -0.8385707576527466 2315 23 -0.8887300112819077 2315 24 -0.8416966230536076 2315 25 -0.9117844486339592 2316 1 -0.8798848377047541 2316 2 -1.0274882882915044 2316 3 -1.0333060758372952 2316 4 -0.8895891925749098 2316 5 -1.1366848890494268 2316 6 -1.036294992265745 2316 7 -1.137631626463488 2316 8 -1.107158825600436 2316 9 -1.4192421608823125 2316 10 -1.4783041728457107 2316 11 -1.440588029774645 2316 12 -1.3502149791356004 2316 13 -1.459714502168147 2316 14 -1.6925777423414843 2316 15 -1.4953567056215036 2316 16 -1.6111281450191584 2316 17 -1.6612502304940904 2316 18 -1.5689794972683444 2316 19 -1.5497847034700811 2316 20 -1.497548380547336 2316 21 -1.7699781374600931 2316 22 -1.7521877416864555 2316 23 -1.9254098536968627 2316 24 -1.864255105693232 2316 25 -2.0697512812328895 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 25769.0 3 0.0 4 470.0 5 4327.0 6 5424.0 7 4423.0 8 3643.0 9 3222.0 10 3136.0 11 2921.0 12 2874.0 13 2685.0 14 2757.0 15 2777.0 16 2826.0 17 3134.0 18 3278.0 19 3688.0 20 4475.0 21 5727.0 22 7329.0 23 9579.0 24 11689.0 25 13954.0 26 16839.0 27 19055.0 28 22567.0 29 27859.0 30 36011.0 31 47096.0 32 61239.0 33 80553.0 34 105917.0 35 140997.0 36 204568.0 37 356352.0 38 923123.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.20507212366567 19.121846106894232 13.347929927101806 25.32515184233829 2 15.379073536919453 20.727962240647283 39.401449995235424 24.49151422719784 3 18.436778265078722 24.71404508528585 30.002996847095893 26.846179802539538 4 13.199799473641328 17.37149349325111 37.172412618429554 32.25629441467802 5 13.963938444702068 37.251723879046814 35.072619167701845 13.71171850854927 6 31.87581912669758 36.49165417213135 18.141927179837985 13.490599521333085 7 28.194438754066574 31.473431408338598 22.237157865710866 18.094971971883957 8 25.748035193423885 35.502968386317434 20.63042301346454 18.11857340679414 9 26.638564128154574 15.567538851981993 20.05746949177432 37.73642752808911 10 15.877351155443375 28.347457490575582 33.41060994354787 22.36458141043317 11 34.19540639962656 22.63457385616883 24.27464561477487 18.89537412942973 12 23.596234928874367 24.489350604870545 30.83101971520285 21.083394751052236 13 28.79288748289242 21.659010359147494 25.743791209524723 23.804310948435354 14 21.837532218408008 21.382112735771535 27.076398425067083 29.703956620753374 15 23.433456874633737 29.496847326062024 23.4465306776327 23.623165121671533 16 23.135843718336883 27.565929485246627 26.45240053897213 22.845826257444358 17 22.54029516411996 27.753428075439523 27.03377046176764 22.67250629867287 18 22.415403517865766 27.1184279396377 28.393998387871193 22.072170154625343 19 23.947535356827522 26.569386479287683 27.098599537260814 22.38447862662398 20 24.057592864281496 26.65329517378721 27.300310318683156 21.98880164324814 21 23.772040751596364 26.15211738065436 26.872603615643083 23.203238252106196 22 23.89822136434341 26.767829053580954 26.82053857623523 22.5134110058404 23 23.26285295240077 26.704577626395825 27.10323654882904 22.929332872374363 24 23.532978266464045 26.353195624842034 27.437455488121802 22.67637062057212 25 23.430473868545395 26.54885630001731 27.155140665338394 22.8655291660989 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 855.0 1 855.0 2 712.0 3 569.0 4 569.0 5 569.0 6 1251.0 7 1933.0 8 1933.0 9 1933.0 10 2694.5 11 3456.0 12 3456.0 13 3456.0 14 5819.0 15 8182.0 16 8182.0 17 8182.0 18 15087.0 19 21992.0 20 21992.0 21 21992.0 22 35010.5 23 48029.0 24 48029.0 25 48029.0 26 68872.0 27 89715.0 28 89715.0 29 89715.0 30 115797.5 31 141880.0 32 141880.0 33 141880.0 34 164893.0 35 187906.0 36 187906.0 37 187906.0 38 208588.0 39 229270.0 40 229270.0 41 229270.0 42 247094.5 43 264919.0 44 264919.0 45 264919.0 46 273127.0 47 281335.0 48 281335.0 49 281335.0 50 278739.0 51 276143.0 52 276143.0 53 276143.0 54 253300.0 55 230457.0 56 230457.0 57 230457.0 58 200085.0 59 169713.0 60 169713.0 61 169713.0 62 139628.5 63 109544.0 64 109544.0 65 109544.0 66 85356.0 67 61168.0 68 61168.0 69 61168.0 70 44884.0 71 28600.0 72 28600.0 73 28600.0 74 19840.0 75 11080.0 76 11080.0 77 11080.0 78 7516.0 79 3952.0 80 3952.0 81 3952.0 82 2570.0 83 1188.0 84 1188.0 85 1188.0 86 751.0 87 314.0 88 314.0 89 314.0 90 188.5 91 63.0 92 63.0 93 63.0 94 40.5 95 18.0 96 18.0 97 18.0 98 10.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.445522061352043 2 0.0 3 0.0 4 0.0 5 0.05349210945351043 6 0.0 7 0.0 8 0.0027160365385173113 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 4.603451760198832E-5 20 0.0 21 0.0 22 0.0 23 0.0 24 0.005063796936218715 25 0.0016112081160695913 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2172283.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.85428315158515 #Duplication Level Percentage of deduplicated Percentage of total 1 80.652723437614 56.33938179957576 2 12.633148120149881 17.64959011761731 3 3.4608038778731665 7.252559220511684 4 1.3145082362060299 3.672961221481071 5 0.6393009397398173 2.2328954431829833 6 0.3693731044727104 1.5481376057050056 7 0.22536498389602802 1.1019896578267898 8 0.14902996809057276 0.8328305271256458 9 0.10454728504219549 0.6572768086860306 >10 0.42303404337084605 4.75103769283748 >50 0.01598574687568168 0.7765779727741777 >100 0.010075154644602664 1.3969967812335395 >500 0.0013156887652947815 0.6472055416647844 >1k 7.894132591761679E-4 1.1405596097777277 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4740 0.21820361343342465 No Hit TATCAACGCAGAGTACTTTTTTTTT 3044 0.14012907158045246 No Hit GGTATCAACGCAGAGTACTTTTTTT 2951 0.13584786144346755 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACATCG 60 4.0887983E-4 12.668279 5 GGTATCA 1330 0.0 12.053155 1 TAGGACC 495 0.0 10.940786 4 GTATAAC 155 3.4069672E-9 10.465508 1 GTATAGG 270 0.0 10.248901 1 GTCCTAC 1195 0.0 9.9812565 1 GTAGGAC 1205 0.0 9.777178 3 GTATCAA 2590 0.0 9.578922 1 GTCGTAG 90 0.0010697487 9.542081 1 GCGCCCA 80 0.004528242 9.497053 19 GTATAAG 215 9.822543E-11 9.320172 1 TCAATAC 165 1.0637632E-7 9.213293 3 TAACCCC 145 1.7036564E-6 9.173581 4 GTCTACG 125 2.6147007E-5 9.160398 1 TCCTACA 1405 0.0 9.129275 2 CCGTCCT 115 1.115146E-4 9.08372 9 TAGAACC 180 4.2104148E-8 8.973364 4 TCTTATA 265 1.8189894E-12 8.963405 2 AGGACGT 1255 0.0 8.933408 5 GTCCTAA 770 0.0 8.922465 1 >>END_MODULE