FastQCFastQC Report
Thu 26 May 2016
SRR1512120_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512120_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences998663
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT71610.7170587074919167No Hit
TATCAACGCAGAGTACTTTTTTTTT47200.4726319088621487No Hit
GGTATCAACGCAGAGTACTTTTTTT45850.45911383519765925No Hit
GTACATAAGCAGTGGTATCAACGCA22600.22630256653145253No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21460.2148873043258837No Hit
GTACTTTTTTTTTTTTTTTTTTTTT20210.20237056945135645No Hit
GAGTACTTTTTTTTTTTTTTTTTTT14860.14879894418837986No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA10320.10333816312409691No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTATAC951.1006287E-611.9424183
CTAGGAC1201.07320375E-811.8180173
TAGGACT1501.9099389E-1011.3452964
TGCTAGA906.226018E-511.0046392
TAGATCT951.433119E-510.9472164
TAGGACA1408.980351E-910.8050444
GTCCTAC650.00781129510.6660351
GCTTAAT750.001871655210.5644541
AGTGCAT856.8831915E-410.01105710
CTTATAC856.88636E-410.0105553
TAGAACA2002.7284841E-119.92713454
ATATAGG907.5956807E-49.9041751
GTCTTAG907.5956807E-49.9041751
GGTTAGG800.00319822049.9041751
TAGACAG1252.728193E-69.8325895
CTATTCC1252.728193E-69.8325894
CAGTGCA1752.7848728E-99.7250269
TCTGCAC1857.057679E-109.7099383
CCTGGAC1951.7826096E-109.6968343
GAATCAA1552.3217763E-79.5846851
Produced by FastQC (version 0.11.5)