##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512120_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 998663 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.62407338611724 31.0 28.0 33.0 16.0 34.0 2 26.029492431380756 31.0 25.0 33.0 10.0 34.0 3 27.699631407191415 31.0 26.0 34.0 16.0 34.0 4 31.99179302727747 35.0 32.0 37.0 19.0 37.0 5 32.39265097435271 35.0 33.0 37.0 22.0 37.0 6 32.65742798121088 35.0 33.0 37.0 25.0 37.0 7 32.70023421314297 36.0 33.0 37.0 25.0 37.0 8 32.73812086759998 36.0 33.0 37.0 25.0 37.0 9 34.22445409512518 39.0 35.0 39.0 26.0 39.0 10 34.08534510640727 38.0 34.0 39.0 25.0 39.0 11 34.09154539619471 38.0 35.0 39.0 25.0 39.0 12 33.98542150855694 38.0 34.0 39.0 25.0 39.0 13 33.9474076840736 38.0 34.0 39.0 25.0 39.0 14 34.94299678670382 39.0 34.0 41.0 24.0 41.0 15 34.832278756697704 39.0 34.0 41.0 24.0 41.0 16 34.79606834337509 39.0 34.0 41.0 24.0 41.0 17 34.76904621478917 39.0 34.0 41.0 23.0 41.0 18 34.72817657207687 39.0 34.0 41.0 23.0 41.0 19 34.76240533593415 39.0 34.0 41.0 22.0 41.0 20 34.69049419073301 39.0 34.0 41.0 22.0 41.0 21 34.62430970207167 39.0 34.0 41.0 20.0 41.0 22 34.503896709901134 39.0 34.0 41.0 19.0 41.0 23 34.36710281646562 39.0 34.0 41.0 18.0 41.0 24 34.22506290910948 39.0 34.0 41.0 17.0 41.0 25 34.07522357391833 39.0 34.0 41.0 16.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.2537454716628815 1101 2 3.6454308279464342 1101 3 2.0893105665907896 1101 4 1.4683545987922102 1101 5 1.0000724270664705 1101 6 0.7005937278137679 1101 7 0.4343842610208739 1101 8 0.731108098734687 1101 9 0.5576384173133135 1101 10 0.27985175973864784 1101 11 0.5996630622382568 1101 12 0.17107403432145674 1101 13 0.43626577161658275 1101 14 0.49597222535602725 1101 15 0.6630962024004532 1101 16 0.551864267946435 1101 17 0.37196515983522715 1101 18 0.397864560504253 1101 19 0.49981691909337655 1101 20 0.5695713630932531 1101 21 0.6019636430052344 1101 22 0.6474879835034244 1101 23 0.9658426869398014 1101 24 0.9845166690386975 1101 25 0.9611351928070704 1102 1 0.6708278884738519 1102 2 3.1511382272069746 1102 3 1.929618050093385 1102 4 0.8546453742871307 1102 5 0.3603160270103061 1102 6 0.12802855722360817 1102 7 0.2053839334128753 1102 8 -0.0362247041856989 1102 9 -0.057721717473974365 1102 10 -0.1410110193131615 1102 11 0.17139868258739455 1102 12 0.1257319179738161 1102 13 -0.01276310468799835 1102 14 -0.21293871207084436 1102 15 0.05890633016756652 1102 16 0.2245979226476038 1102 17 -0.22980509335895505 1102 18 -0.15313464387633502 1102 19 -0.10520133335198523 1102 20 -0.11527091705838188 1102 21 0.06711317265375527 1102 22 0.05423436811521043 1102 23 -0.1468357424138702 1102 24 0.02277777260962921 1102 25 -0.17127342796327838 1103 1 1.274273815086108 1103 2 3.2915232880454965 1103 3 2.2517669966036316 1103 4 1.5348332265669242 1103 5 1.1345844432102936 1103 6 0.8913385452784581 1103 7 0.9044081098559502 1103 8 0.6234122631082002 1103 9 0.7276164030040135 1103 10 0.6328191051798484 1103 11 0.7902381860687484 1103 12 0.610309954336131 1103 13 0.8213877675806245 1103 14 0.6518335352074303 1103 15 0.7332805722408509 1103 16 0.2981310079941295 1103 17 0.9570275337115817 1103 18 0.7303081488366701 1103 19 0.8958864476202493 1103 20 0.9630175197617561 1103 21 0.96066846780802 1103 22 0.9843711242115489 1103 23 1.175290494681505 1103 24 1.0766694850357652 1103 25 1.0179550423759594 1104 1 1.1529305295898453 1104 2 3.671744646720935 1104 3 2.2056292309668066 1104 4 1.5137105302489715 1104 5 1.1345587933058425 1104 6 0.9608465801110242 1104 7 0.7728208937683263 1104 8 0.5891407845194578 1104 9 0.7378859834998224 1104 10 0.8334077704870708 1104 11 0.8832992456663007 1104 12 0.7427019361759974 1104 13 0.8467234607051637 1104 14 0.9629604637596216 1104 15 0.6961266856390722 1104 16 0.755507279267519 1104 17 0.7830891191321712 1104 18 0.9865346631428977 1104 19 0.9326491731791009 1104 20 0.8016283209365156 1104 21 0.7991157508418922 1104 22 0.8755770544694599 1104 23 1.0059818878560591 1104 24 1.0835244220011262 1104 25 1.1288139294265989 1105 1 0.7720467861219333 1105 2 3.325056712678485 1105 3 1.6332438325159657 1105 4 1.178794437012634 1105 5 0.7399864816670743 1105 6 0.5630810887844504 1105 7 0.04778163210276887 1105 8 0.2556783717377229 1105 9 0.18675874087246314 1105 10 0.01137349886907657 1105 11 -0.03388092158378697 1105 12 -0.07794012139138573 1105 13 0.17357618617674575 1105 14 0.11973360048241943 1105 15 0.04674129845707142 1105 16 -0.13762005965720903 1105 17 0.12760216691308557 1105 18 -0.04440035355035121 1105 19 0.5316925510448414 1105 20 0.24027409312358827 1105 21 0.310709851296437 1105 22 0.0900056781444718 1105 23 0.1941541126223143 1105 24 0.011993918785918822 1105 25 0.3051948490254688 1106 1 0.3059193847063604 1106 2 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0.43126429563294266 1107 14 0.6313114907378861 1107 15 0.6735008327719783 1107 16 0.9381797910772036 1107 17 0.8712703932514927 1107 18 0.7119623127920249 1107 19 0.9074171805533311 1107 20 0.9054641223392323 1107 21 0.9458319852647534 1107 22 1.1341008119097822 1107 23 1.2060316128454218 1107 24 1.1675586713159305 1107 25 1.329295062920508 1108 1 0.6892210924862461 1108 2 2.1909179989719263 1108 3 1.3033646875379041 1108 4 1.001117984651458 1108 5 0.6845848435337558 1108 6 0.710782656120017 1108 7 0.4980217496769228 1108 8 0.5629454058875609 1108 9 0.5176087167935535 1108 10 0.7217109297741189 1108 11 0.7494712463814821 1108 12 0.8213524766941944 1108 13 0.6917149919779391 1108 14 0.7368021898804074 1108 15 0.8312197070617842 1108 16 0.7491994713124939 1108 17 0.8753415314217321 1108 18 0.7963795345162978 1108 19 0.8095046511286839 1108 20 0.6825509027351941 1108 21 1.115415503412791 1108 22 0.9541747255214119 1108 23 0.7905436192061188 1108 24 0.790438210165668 1108 25 0.8871293352045626 1109 1 1.1370638499971655 1109 2 3.4625162345864524 1109 3 1.9865656444927389 1109 4 1.5065204361361992 1109 5 1.1001641963788131 1109 6 0.5838223761537691 1109 7 0.6264594710706319 1109 8 0.39554239190704976 1109 9 0.3761962115869082 1109 10 0.3759219938523586 1109 11 0.5816089795235158 1109 12 0.6228217298920598 1109 13 0.661137294579305 1109 14 0.6326799502928111 1109 15 0.5187675459031809 1109 16 0.38827833108371834 1109 17 0.49844978337711154 1109 18 0.5512702216511656 1109 19 0.33033123510610096 1109 20 0.5008932490545845 1109 21 0.7054753486908467 1109 22 0.48088793455978873 1109 23 0.3957961904882197 1109 24 0.41056487851744805 1109 25 0.3660642245388601 1110 1 0.2004147975503976 1110 2 1.6987814462152784 1110 3 0.9087561190439217 1110 4 0.33806939735622876 1110 5 0.3157983943580547 1110 6 0.429113871411694 1110 7 0.3335665506100227 1110 8 0.2378492235851084 1110 9 0.19353789876524985 1110 10 0.2062677948403575 1110 11 0.22853222857251865 1110 12 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-1.2791428559575415 2316 2 -1.9492561646568518 2316 3 -1.2538880623105264 2316 4 -1.5296834672370174 2316 5 -1.6457646647610602 2316 6 -1.6097075530810514 2316 7 -1.613035908562388 2316 8 -1.8337484771267576 2316 9 -1.7708840434480067 2316 10 -2.1127470134888497 2316 11 -2.40295735991581 2316 12 -2.3519324603565153 2316 13 -2.134977237584213 2316 14 -2.7363748791000404 2316 15 -2.626192089138428 2316 16 -2.6611070472258547 2316 17 -2.890893400511157 2316 18 -2.4805406352381283 2316 19 -2.87691499503255 2316 20 -2.876834013056847 2316 21 -2.9823318648425037 2316 22 -2.8334519407253325 2316 23 -2.9192015129519504 2316 24 -2.6418363888721785 2316 25 -3.367418839305504 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 46501.0 3 1.0 4 385.0 5 1894.0 6 2221.0 7 2019.0 8 1941.0 9 1820.0 10 1782.0 11 1693.0 12 1764.0 13 1749.0 14 1712.0 15 1832.0 16 1828.0 17 2078.0 18 2449.0 19 2935.0 20 4047.0 21 4933.0 22 6139.0 23 7289.0 24 8513.0 25 9554.0 26 10918.0 27 12407.0 28 14503.0 29 17432.0 30 21806.0 31 26994.0 32 34586.0 33 44031.0 34 58198.0 35 78206.0 36 111083.0 37 151999.0 38 299421.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.18797932836202 18.941024149287596 13.396911670904 23.474084851446385 2 16.6498129658807 22.191918195912734 38.262166658624444 22.896102179582126 3 18.272129837592864 26.023693678448083 30.41816909207611 25.286007391882947 4 12.559091505342643 17.569890944192384 37.87944481772129 31.991572732743677 5 13.218773500169728 38.212189697625725 34.675661359237296 13.893375442967248 6 30.31412999179903 37.04162465215994 18.693192798771957 13.951052557269069 7 26.912181586781525 33.47115092879179 22.16493451744983 17.45173296697685 8 25.867963070514488 34.32709831173773 21.169767488434502 18.63517112931328 9 26.99609377738036 15.57532420846672 20.546871166749945 36.88171084740298 10 16.134672056539593 28.538956584954082 32.755694363363816 22.570676995142506 11 32.735367185927586 24.098920256382783 23.85519439490599 19.31051816278364 12 23.45405807564714 25.77305857932055 30.417067619407145 20.355815725625163 13 28.138320935090217 22.38603012227348 26.78230794572343 22.693340996912873 14 22.590183797207246 21.83847274584316 28.08176998062394 27.489573476325656 15 23.942411003511697 28.637388188007368 25.085038696737538 22.3351621117434 16 22.461931602552614 28.50330892403143 26.82206109568493 22.212698377731027 17 21.560726691586652 28.262486945045527 27.722865471134906 22.453920892232915 18 21.18912986663169 27.442791011582486 29.6425320653714 21.725547056414428 19 22.451517679137005 27.417957809591424 29.245601369030393 20.884923142241178 20 23.000975304958036 26.717147543413088 29.138287028043525 21.14359012358536 21 23.30235524896787 26.601666428014255 28.726507340314 21.369470982703874 22 23.028226908738436 26.374120443755967 28.743602658370193 21.85404998913541 23 22.967322146320363 26.411043620538766 28.667171343943572 21.954462889197302 24 22.17399097391407 26.645378161358057 28.787346256637637 22.393284608090234 25 22.56106414275887 26.851400322230823 28.752842550490005 21.834692984520306 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2132.0 1 2132.0 2 1653.0 3 1174.0 4 1174.0 5 1174.0 6 2203.0 7 3232.0 8 3232.0 9 3232.0 10 3737.0 11 4242.0 12 4242.0 13 4242.0 14 5412.0 15 6582.0 16 6582.0 17 6582.0 18 10910.0 19 15238.0 20 15238.0 21 15238.0 22 21838.0 23 28438.0 24 28438.0 25 28438.0 26 39311.0 27 50184.0 28 50184.0 29 50184.0 30 60247.0 31 70310.0 32 70310.0 33 70310.0 34 77924.5 35 85539.0 36 85539.0 37 85539.0 38 93159.5 39 100780.0 40 100780.0 41 100780.0 42 111153.5 43 121527.0 44 121527.0 45 121527.0 46 123296.0 47 125065.0 48 125065.0 49 125065.0 50 123846.5 51 122628.0 52 122628.0 53 122628.0 54 111242.0 55 99856.0 56 99856.0 57 99856.0 58 85589.0 59 71322.0 60 71322.0 61 71322.0 62 58954.5 63 46587.0 64 46587.0 65 46587.0 66 35978.5 67 25370.0 68 25370.0 69 25370.0 70 18378.0 71 11386.0 72 11386.0 73 11386.0 74 8027.5 75 4669.0 76 4669.0 77 4669.0 78 3174.5 79 1680.0 80 1680.0 81 1680.0 82 1087.0 83 494.0 84 494.0 85 494.0 86 332.5 87 171.0 88 171.0 89 171.0 90 107.0 91 43.0 92 43.0 93 43.0 94 27.5 95 12.0 96 12.0 97 12.0 98 7.0 99 2.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.542172885147442 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 3.004016369886538E-4 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 8.010710319697436E-4 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 1.0013387899621795E-4 21 0.0 22 6.008032739773077E-4 23 5.006693949810897E-4 24 2.002677579924359E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 998663.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.46006869137675 #Duplication Level Percentage of deduplicated Percentage of total 1 78.47851789152772 52.15687689868373 2 13.390767424279057 17.799026456956728 3 4.251180387196415 8.47601221657522 4 1.6660924672094026 4.429144792676891 5 0.8027834131106262 2.667652038981505 6 0.4508096562299113 1.797650443186551 7 0.24717625214159708 1.1499145487345321 8 0.15257399127322674 0.8112062352428935 9 0.11149137214574968 0.6668751826172135 >10 0.4195623854088988 4.516258321633667 >50 0.017124298013091142 0.7978124923964646 >100 0.008863933293718984 1.2382221420911335 >500 0.0018339169023739328 0.912390159681861 >1k 0.0010697848597181275 1.8536257836128203 >5k 1.5282640853116105E-4 0.7273322869288441 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 7161 0.7170587074919167 No Hit TATCAACGCAGAGTACTTTTTTTTT 4720 0.4726319088621487 No Hit GGTATCAACGCAGAGTACTTTTTTT 4585 0.45911383519765925 No Hit GTACATAAGCAGTGGTATCAACGCA 2260 0.22630256653145253 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2146 0.2148873043258837 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2021 0.20237056945135645 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1486 0.14879894418837986 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 1032 0.10333816312409691 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 2.002677579924359E-4 0.0 8 0.0 0.0 0.0 2.002677579924359E-4 0.0 9 0.0 0.0 0.0 2.002677579924359E-4 0.0 10 0.0 0.0 0.0 2.002677579924359E-4 0.0 11 0.0 0.0 0.0 2.002677579924359E-4 0.0 12 0.0 0.0 0.0 2.002677579924359E-4 0.0 13 0.0 0.0 0.0 2.002677579924359E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTATAC 95 1.1006287E-6 11.942418 3 CTAGGAC 120 1.07320375E-8 11.818017 3 TAGGACT 150 1.9099389E-10 11.345296 4 TGCTAGA 90 6.226018E-5 11.004639 2 TAGATCT 95 1.433119E-5 10.947216 4 TAGGACA 140 8.980351E-9 10.805044 4 GTCCTAC 65 0.007811295 10.666035 1 GCTTAAT 75 0.0018716552 10.564454 1 AGTGCAT 85 6.8831915E-4 10.011057 10 CTTATAC 85 6.88636E-4 10.010555 3 TAGAACA 200 2.7284841E-11 9.9271345 4 ATATAGG 90 7.5956807E-4 9.904175 1 GTCTTAG 90 7.5956807E-4 9.904175 1 GGTTAGG 80 0.0031982204 9.904175 1 TAGACAG 125 2.728193E-6 9.832589 5 CTATTCC 125 2.728193E-6 9.832589 4 CAGTGCA 175 2.7848728E-9 9.725026 9 TCTGCAC 185 7.057679E-10 9.709938 3 CCTGGAC 195 1.7826096E-10 9.696834 3 GAATCAA 155 2.3217763E-7 9.584685 1 >>END_MODULE