FastQCFastQC Report
Thu 26 May 2016
SRR1512120_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512120_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences998663
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT93870.9399567221374978No Hit
TATCAACGCAGAGTACTTTTTTTTT63050.6313441070711541No Hit
GGTATCAACGCAGAGTACTTTTTTT60780.6086137165390126No Hit
ACGCAGAGTACTTTTTTTTTTTTTT29690.2972974867397711No Hit
GTACTTTTTTTTTTTTTTTTTTTTT26720.26755772467789435No Hit
GTACATAAGCAGTGGTATCAACGCA23660.23691675770505163No Hit
GAGTACTTTTTTTTTTTTTTTTTTT20110.20136923066139426No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT11750.1176573078205561No Hit
GCAGAGTACTTTTTTTTTTTTTTTT11720.11735690618356744No Hit
GCTTATGTACTCTGCGTTGATACCA11370.11385222041869979No Hit
GTGGTATCAACGCAGAGTACATGGG10130.10143561942316877No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGACGA350.002172259116.28258118
CGACGAC400.00528409514.24725819
CACGACG400.00528936414.24511817
CCCTAGG500.001498803113.3001052
TAAGGCC500.001498803113.3001054
TAGGCTA658.0184813E-411.69245
ACTATAC600.00587281911.0834213
CTATACA1252.2356107E-710.6400854
GGACAGT1252.2464337E-710.6363556
GGTATCA23200.010.5297791
CTTCTAA1605.7170837E-910.1390261
CCTTATA750.002645979910.1334132
ATAAGAC856.588066E-410.0589033
ATCTAGA1053.9033905E-59.9970231
GTACATA6700.09.96989251
TGTATAC1151.02486465E-59.9131213
CGCAGAA1151.0291438E-59.9096479
GTATTGG1106.501711E-59.5426121
GTCTTAC800.00434943439.5426121
TTAGGAC800.0045127689.5000753
Produced by FastQC (version 0.11.5)