##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512120_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 998663 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.963799600065286 31.0 31.0 34.0 28.0 34.0 2 31.1339330685126 31.0 31.0 34.0 28.0 34.0 3 31.217493789196155 31.0 31.0 34.0 27.0 34.0 4 34.831485696376056 37.0 35.0 37.0 32.0 37.0 5 34.46238420768567 37.0 35.0 37.0 30.0 37.0 6 34.42103392235419 37.0 35.0 37.0 30.0 37.0 7 34.25546155209515 37.0 35.0 37.0 30.0 37.0 8 34.169048017198996 37.0 35.0 37.0 30.0 37.0 9 35.6618549000013 39.0 35.0 39.0 30.0 39.0 10 35.501882016255735 39.0 35.0 39.0 30.0 39.0 11 35.48508455805412 39.0 35.0 39.0 30.0 39.0 12 35.32492943064878 39.0 35.0 39.0 30.0 39.0 13 35.26991087083431 39.0 35.0 39.0 29.0 39.0 14 36.291552806101755 40.0 36.0 41.0 29.0 41.0 15 36.19020029779816 40.0 36.0 41.0 29.0 41.0 16 36.09875803949881 39.0 36.0 41.0 28.0 41.0 17 36.089634841783464 39.0 36.0 41.0 28.0 41.0 18 36.04693875711827 39.0 36.0 41.0 28.0 41.0 19 36.08649764735451 39.0 36.0 41.0 28.0 41.0 20 35.99632308396326 39.0 36.0 41.0 28.0 41.0 21 35.8868557261058 39.0 36.0 41.0 27.0 41.0 22 35.7696119712055 39.0 36.0 41.0 27.0 41.0 23 35.80663647296436 39.0 36.0 41.0 27.0 41.0 24 35.69021882256577 39.0 36.0 41.0 27.0 41.0 25 35.2721758991772 39.0 35.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.20629021434533357 1101 2 0.31962033020267455 1101 3 0.2564789399885363 1101 4 0.24750182709826873 1101 5 0.4358221150932806 1101 6 0.31807149862576267 1101 7 0.4244512811280501 1101 8 0.5202883337110435 1101 9 0.5002924018006496 1101 10 0.48662246924681085 1101 11 0.33790469277097657 1101 12 0.29651465972080615 1101 13 0.25078992188131366 1101 14 0.23321101643288955 1101 15 0.2780992445714716 1101 16 0.226736778639669 1101 17 0.34488609506396983 1101 18 0.32114933414084135 1101 19 0.2564731752869207 1101 20 0.3032609903630501 1101 21 -0.029516033543167453 1101 22 0.07876325808071982 1101 23 0.2086184717593227 1101 24 -0.34662111081939884 1101 25 -0.1827866076792617 1102 1 -0.17578377826008307 1102 2 -0.23623800274064877 1102 3 -0.2338753446560098 1102 4 -0.15290534283667512 1102 5 -0.28360152884362577 1102 6 -0.16973329381116997 1102 7 -0.11341903290761479 1102 8 -0.2694762565449622 1102 9 -0.27031882111319305 1102 10 -0.08003054708678548 1102 11 -0.22173400241318575 1102 12 -0.0869633671430492 1102 13 -0.018526079429122433 1102 14 -0.09012637299941417 1102 15 -0.23295986527080714 1102 16 -0.32270745779970156 1102 17 -0.0012376003541945124 1102 18 0.07675728097473922 1102 19 -0.22017422490964123 1102 20 0.06580135634798268 1102 21 0.09368095246328778 1102 22 0.09564677003841382 1102 23 -0.10649829708984271 1102 24 -0.07083602166321867 1102 25 -0.15553430272301938 1103 1 0.23893101419857388 1103 2 0.2682857127012994 1103 3 0.26584419407035753 1103 4 0.3360635588906007 1103 5 0.43586339192322043 1103 6 0.3388429167141922 1103 7 0.23585744513465556 1103 8 0.312675051744435 1103 9 0.30735532603473104 1103 10 0.19410274809472838 1103 11 0.3678716713070287 1103 12 0.33296210055734576 1103 13 0.33500841390112157 1103 14 0.4370726931894495 1103 15 0.4396475843256482 1103 16 0.5509112420408826 1103 17 0.4343880213160318 1103 18 0.6243459953039263 1103 19 0.5425078478240692 1103 20 0.7007293447934302 1103 21 0.6481210972585174 1103 22 0.7455675141805216 1103 23 0.6692587212548275 1103 24 0.7232009401804191 1103 25 0.5943918916786544 1104 1 0.25948941261853875 1104 2 0.09297502823359594 1104 3 0.08445127600897706 1104 4 0.12664812021311178 1104 5 0.2806846762139372 1104 6 0.35922497204103365 1104 7 0.33048956138001984 1104 8 0.20031564528321866 1104 9 0.2600408710310802 1104 10 0.1713929639386791 1104 11 0.35123450203047923 1104 12 0.5097508607692163 1104 13 0.3858753806716706 1104 14 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-1.3083598266562362 2314 24 -1.2778593776963234 2314 25 -1.3749244765686441 2315 1 -0.5130549675380927 2315 2 -0.5906646771633213 2315 3 -0.6640287470888637 2315 4 -0.6639740162137855 2315 5 -0.7667644825722633 2315 6 -0.7601619845457677 2315 7 -0.6767584419860668 2315 8 -0.991217729069902 2315 9 -1.0598386751456772 2315 10 -0.9415241329516562 2315 11 -1.0086446216248248 2315 12 -0.9323685420319237 2315 13 -0.8772093604063045 2315 14 -1.285553385032756 2315 15 -1.3600679446401784 2315 16 -1.3737250126193459 2315 17 -1.2222341784222266 2315 18 -1.434453544263576 2315 19 -1.2228255441630793 2315 20 -1.2114218940852552 2315 21 -1.222782758130485 2315 22 -1.3429777610708626 2315 23 -1.1068312317121425 2315 24 -1.2296982082394123 2315 25 -1.2889438244661733 2316 1 -0.875574299455856 2316 2 -0.8646887082241719 2316 3 -1.0586572678411592 2316 4 -0.9416917503868518 2316 5 -1.0241318809377447 2316 6 -1.1085431504812178 2316 7 -1.1984379485309802 2316 8 -1.2621940765361188 2316 9 -1.2574651302217248 2316 10 -1.2995357483248995 2316 11 -1.4416009900722457 2316 12 -1.2304157028987177 2316 13 -1.4724420824484739 2316 14 -1.6002997178265375 2316 15 -1.4805513056720798 2316 16 -1.6153658733538379 2316 17 -1.5024574012376704 2316 18 -1.5352325890054743 2316 19 -1.2933238660264479 2316 20 -1.5540004204253322 2316 21 -1.460843834373044 2316 22 -1.3555232293809425 2316 23 -1.507185244639743 2316 24 -1.295331195611027 2316 25 -1.3456767393768914 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14516.0 3 0.0 4 268.0 5 2320.0 6 2860.0 7 2368.0 8 1893.0 9 1673.0 10 1618.0 11 1632.0 12 1466.0 13 1461.0 14 1475.0 15 1380.0 16 1458.0 17 1495.0 18 1634.0 19 1851.0 20 2320.0 21 3026.0 22 3824.0 23 4872.0 24 6005.0 25 7315.0 26 8572.0 27 9731.0 28 11220.0 29 13668.0 30 17735.0 31 22942.0 32 29945.0 33 38898.0 34 51320.0 35 68003.0 36 99019.0 37 171696.0 38 387184.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.09105740612776 19.652543711155836 14.055564440447919 23.200834442268484 2 15.439041999152867 21.694605687804593 39.2826208640953 23.583731448947244 3 18.02059353355436 26.409910049736496 30.977717207906974 24.59177920880217 4 12.762964082978941 18.0010674271501 37.624904497312905 31.61106399255805 5 13.082932312973222 38.29195885295653 34.77700662973572 13.848102204334525 6 30.315031196710002 37.40661264110115 18.477304155656114 13.801052006532736 7 26.7118136949101 33.555363521027616 22.26797227893694 17.464850505125355 8 25.896407560395545 34.54199524345975 21.029615721617223 18.531981474527477 9 26.985079050690775 15.462573460716978 20.48348642134534 37.068861067246914 10 15.994684893702882 28.75134054230506 32.86974685154051 22.384227712451548 11 32.8675439062026 23.966843669986773 23.842777793910457 19.322834629900175 12 23.289538112456356 25.977431826351832 30.472942323887036 20.260087737304776 13 28.11068398448726 22.364000668894313 26.90867690101666 22.61663844560177 14 22.485763465753713 21.764298867585964 28.349603419772233 27.400334246888093 15 23.741041772850302 28.66742835170623 25.213410329610692 22.378119545832778 16 22.40505555928276 28.51792847036488 26.96164772300566 22.115368247346705 17 21.405118643626526 28.209315855298534 27.960983835387914 22.424581665687025 18 21.181719959585966 27.271862480135944 29.88185203617236 21.66456552410573 19 22.3337826011203 27.175460766505584 29.651473671772834 20.839282960601285 20 22.835531105087504 26.559510064956847 29.65424772921396 20.950711100741692 21 23.22635363480974 26.390684344969223 29.15197619216893 21.230985828052106 22 22.903421875046938 26.17249262263647 29.154779940780823 21.769305561535774 23 22.73359481626935 26.19372100498366 29.206148620705886 21.8665355580411 24 22.028619781496282 26.400296412012697 29.273990847277716 22.29709295921331 25 22.38823456372277 26.630244308839696 29.220731104066072 21.760790023371456 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2254.0 1 2254.0 2 1740.0 3 1226.0 4 1226.0 5 1226.0 6 2398.0 7 3570.0 8 3570.0 9 3570.0 10 4045.0 11 4520.0 12 4520.0 13 4520.0 14 5510.5 15 6501.0 16 6501.0 17 6501.0 18 11027.0 19 15553.0 20 15553.0 21 15553.0 22 22483.5 23 29414.0 24 29414.0 25 29414.0 26 40420.0 27 51426.0 28 51426.0 29 51426.0 30 61386.0 31 71346.0 32 71346.0 33 71346.0 34 79049.0 35 86752.0 36 86752.0 37 86752.0 38 93844.5 39 100937.0 40 100937.0 41 100937.0 42 111340.5 43 121744.0 44 121744.0 45 121744.0 46 122903.5 47 124063.0 48 124063.0 49 124063.0 50 123128.0 51 122193.0 52 122193.0 53 122193.0 54 110628.0 55 99063.0 56 99063.0 57 99063.0 58 84832.5 59 70602.0 60 70602.0 61 70602.0 62 58089.0 63 45576.0 64 45576.0 65 45576.0 66 35022.5 67 24469.0 68 24469.0 69 24469.0 70 17804.5 71 11140.0 72 11140.0 73 11140.0 74 7728.5 75 4317.0 76 4317.0 77 4317.0 78 2881.0 79 1445.0 80 1445.0 81 1445.0 82 928.0 83 411.0 84 411.0 85 411.0 86 263.0 87 115.0 88 115.0 89 115.0 90 67.5 91 20.0 92 20.0 93 20.0 94 12.5 95 5.0 96 5.0 97 5.0 98 3.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4471979035971093 2 0.0 3 0.0 4 0.0 5 0.058478185333791274 6 0.0 7 0.0 8 0.0038050874018562817 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 1.0013387899621795E-4 20 0.0 21 0.0 22 0.0 23 0.0 24 0.005307095586799551 25 9.012049109659615E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 998663.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.597505765608 #Duplication Level Percentage of deduplicated Percentage of total 1 76.02815973546552 47.5917316738937 2 14.410171178052916 18.04081546803131 3 4.8298538211987925 9.070104072586057 4 1.9856303428243274 4.971820273332481 5 0.9781693376128389 3.0615480375480315 6 0.5362731073238407 2.014161535658678 7 0.33382739946896084 1.462773379408334 8 0.20204451004669807 1.0117985906046103 9 0.13457230660466502 0.7581501664719017 >10 0.5282775839569677 5.41266604586028 >50 0.01930577748946043 0.8372838767940782 >100 0.01036591362369851 1.3665417638460515 >500 0.0015947553958258745 0.7783566555323135 >1k 0.0012758043166606996 1.4489992118829715 >5k 4.7842661874776234E-4 2.173249248549245 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 9387 0.9399567221374978 No Hit TATCAACGCAGAGTACTTTTTTTTT 6305 0.6313441070711541 No Hit GGTATCAACGCAGAGTACTTTTTTT 6078 0.6086137165390126 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2969 0.2972974867397711 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2672 0.26755772467789435 No Hit GTACATAAGCAGTGGTATCAACGCA 2366 0.23691675770505163 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 2011 0.20136923066139426 No Hit TTTTTTTTTTTTTTTTTTTTTTTTT 1175 0.1176573078205561 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 1172 0.11735690618356744 No Hit GCTTATGTACTCTGCGTTGATACCA 1137 0.11385222041869979 No Hit GTGGTATCAACGCAGAGTACATGGG 1013 0.10143561942316877 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 1.0013387899621795E-4 9 0.0 0.0 0.0 0.0 1.0013387899621795E-4 10 0.0 0.0 0.0 0.0 1.0013387899621795E-4 11 0.0 0.0 0.0 0.0 1.0013387899621795E-4 12 0.0 0.0 0.0 2.002677579924359E-4 1.0013387899621795E-4 13 0.0 0.0 0.0 2.002677579924359E-4 1.0013387899621795E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACGACGA 35 0.0021722591 16.282581 18 CGACGAC 40 0.005284095 14.247258 19 CACGACG 40 0.005289364 14.245118 17 CCCTAGG 50 0.0014988031 13.300105 2 TAAGGCC 50 0.0014988031 13.300105 4 TAGGCTA 65 8.0184813E-4 11.6924 5 ACTATAC 60 0.005872819 11.083421 3 CTATACA 125 2.2356107E-7 10.640085 4 GGACAGT 125 2.2464337E-7 10.636355 6 GGTATCA 2320 0.0 10.529779 1 CTTCTAA 160 5.7170837E-9 10.139026 1 CCTTATA 75 0.0026459799 10.133413 2 ATAAGAC 85 6.588066E-4 10.058903 3 ATCTAGA 105 3.9033905E-5 9.997023 1 GTACATA 670 0.0 9.9698925 1 TGTATAC 115 1.02486465E-5 9.913121 3 CGCAGAA 115 1.0291438E-5 9.909647 9 GTATTGG 110 6.501711E-5 9.542612 1 GTCTTAC 80 0.0043494343 9.542612 1 TTAGGAC 80 0.004512768 9.500075 3 >>END_MODULE