FastQCFastQC Report
Thu 26 May 2016
SRR1512119_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512119_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2678645
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT44080.1645608133963254No Hit
GGTATCAACGCAGAGTACTTTTTTT29510.11016764072880132No Hit
TATCAACGCAGAGTACTTTTTTTTT28780.10744238224923422No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTTAA550.002241144712.6087241
GTATTGG2400.011.1452111
GCGCCCG803.9450062E-410.6359259
GTCCTAC16100.010.399121
GGTATCA13850.010.3002671
GTATTAA3300.010.2070621
GTCCTAA7950.010.0937771
CGCCCGC750.0027570610.08443310
GGACGTG14200.010.053556
AGGACGT14800.09.9653775
TAGGACG16050.09.8961234
GCACCGT1151.08753975E-59.865396
GACGTGA8350.09.8506217
TCCTACA17700.09.7391312
TCCAACG2253.6379788E-129.66442918
TAGGACC6100.09.6093214
GTATCAA25500.09.55721
GACGTGG7150.09.5204467
GTCCTAG4500.09.466551
ACCGTGG1804.5474735E-99.4543318
Produced by FastQC (version 0.11.5)