FastQCFastQC Report
Thu 26 May 2016
SRR1512119_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512119_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2678645
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT55620.20764229675824902No Hit
TATCAACGCAGAGTACTTTTTTTTT36870.1376442193721079No Hit
GGTATCAACGCAGAGTACTTTTTTT35180.1313350593303704No Hit
GTCCTACAGTGGACATTTCTAAATT27180.10146921297895019No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGCGA250.0060466318.99268217
TAGGACC6250.012.1623554
GGTATCA18400.010.7348811
AGGACGT15600.010.1109325
TGGCGAG17000.010.05588718
GGACGTG15000.010.0028136
TTATACT3350.09.9272954
TTAGACG1151.0246669E-59.9149634
GTCCTAC15350.09.8218551
GACGTGG7050.09.6983917
GTCCTAA8850.09.5960281
ATGGCGA17750.09.57659217
AGGACCT12900.09.5754975
GGCGAGG9050.09.54987719
GTAGGAC16950.09.5298683
CTACACG1201.6974009E-59.501844
GTTATAC1606.620394E-89.501843
TAGTATC800.0045100419.501844
TATGGCG17700.09.38903816
TTAGGAC11450.09.3773613
Produced by FastQC (version 0.11.5)