FastQCFastQC Report
Thu 26 May 2016
SRR1512118_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512118_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2859902
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT53000.18532103547604079No Hit
TATCAACGCAGAGTACTTTTTTTTT34070.1191299562012964No Hit
GGTATCAACGCAGAGTACTTTTTTT33310.1164725224850362No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTC500.001553874513.2379537
TAGGACC8750.012.535534
GGTATCA15200.011.26073551
GTACCGT1105.2829455E-711.1748956
GGCGAGG8200.010.72431319
CTAATAC2300.010.6890313
GTCCTAA10050.010.6321711
GTAGGAC20600.010.4196093
CGGTAGG1052.6265818E-510.3649611
TTAGGAC11500.010.2779133
GTATCAA29950.010.2406871
GTCCTAC14700.010.2303511
GGACGTG17600.010.1541126
AGGACGT17900.010.14240555
TGTAGGA22000.010.0737132
GTCGTAT800.00322930479.8938261
TGGCGAG18050.09.84874718
CTGTAGG22300.09.6719911
TAGGACG18800.09.55627254
GACGTGA10000.09.5502377
Produced by FastQC (version 0.11.5)