FastQCFastQC Report
Thu 26 May 2016
SRR1512118_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512118_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2859902
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69280.24224606297698315No Hit
GGTATCAACGCAGAGTACTTTTTTT46250.1617188281276771No Hit
TATCAACGCAGAGTACTTTTTTTTT46020.1609146047661773No Hit
GTCCTACAGTGGACATTTCTAAATT31270.10933941093086406No Hit
CTGTAGGACGTGGAATATGGCAAGA31060.10860512003558163No Hit
GTCCTAAAGTGTGTATTTCTCATTT29250.1022762318429093No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG701.0892696E-412.2169855
GGTATCA17000.011.7304231
TAAGACG600.00586804611.0857824
TATTCCG951.35786195E-511.002435
TATGTCG1659.367795E-1010.35958316
TGGCGAG17500.010.25670518
TAGGACC7100.010.1712614
TCCAACG2550.010.05559318
GGCGAGG7950.010.03503919
CTAGTAC1901.0004442E-1010.002213
TGTAGGA19400.09.9918982
GTATCAA34300.09.8753051
CCAACGT1356.471673E-79.84920519
GTAGGAC18200.09.8153553
GTCCTAA11100.09.799371
GTGTAGG4200.09.768671
ATGGCGA18900.09.69726417
TATGGCG19150.09.62025716
AGGACGT18350.09.5279915
TAGGACT4000.09.5020994
Produced by FastQC (version 0.11.5)