FastQCFastQC Report
Thu 26 May 2016
SRR1512117_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512117_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2424096
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT57650.23782061436510765No Hit
TATCAACGCAGAGTACTTTTTTTTT36610.1510253719324647No Hit
GGTATCAACGCAGAGTACTTTTTTT34780.143476165960424No Hit
GTCCTACAGTGGACATTTCTAAATT32930.13584445500508233No Hit
CTTTAGGACGTGAAATATGGCGAGG31710.13081165102372183No Hit
GTCCTAAAGTGTGTATTTCTCATTT31190.12866652145789606No Hit
CTGTAGGACGTGGAATATGGCAAGA29720.1226024051852732No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGA500.001553713113.2380016
TCCAACG1600.013.00160818
CCAACGA1155.7134457E-912.33354119
TAGGACC6650.012.2284434
CGTTAGG655.705512E-412.177071
GGTATCA14650.012.1562891
TACACCG1104.0738087E-812.0345465
GTATTAG2000.011.377951
CCCGTTT1355.0913513E-911.20677312
TTAGGAC13350.010.7660573
GGCGAGG9800.010.71004419
AGGACGT20500.010.7011015
CTAGTAC1159.3693416E-710.6890693
GGACGTG19950.010.4747516
GACGTGA11250.010.4222987
TAGGACG22350.010.2384024
TCCCGTT1502.6304406E-810.08609611
GGTCTAG1302.525352E-69.8938681
GACCTAC1603.543755E-89.8938681
GTCCTAA13050.09.704331
Produced by FastQC (version 0.11.5)