##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512117_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2424096 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.576176026031973 31.0 28.0 33.0 16.0 34.0 2 26.06717431982892 31.0 25.0 33.0 10.0 34.0 3 27.72135963262181 31.0 26.0 34.0 16.0 34.0 4 31.967117226380473 35.0 32.0 37.0 19.0 37.0 5 32.347678887304795 35.0 33.0 37.0 22.0 37.0 6 32.58979553615038 35.0 33.0 37.0 25.0 37.0 7 32.62068457684844 36.0 33.0 37.0 25.0 37.0 8 32.653473707311925 36.0 33.0 37.0 25.0 37.0 9 34.15330127189682 38.0 35.0 39.0 25.0 39.0 10 33.99406995432524 38.0 34.0 39.0 25.0 39.0 11 34.00360381767059 38.0 34.0 39.0 25.0 39.0 12 33.889881011313086 38.0 34.0 39.0 24.0 39.0 13 33.87778784338574 38.0 34.0 39.0 24.0 39.0 14 34.893790922471716 39.0 34.0 41.0 24.0 41.0 15 34.82358701965599 39.0 34.0 41.0 23.0 41.0 16 34.78415541298695 39.0 34.0 41.0 23.0 41.0 17 34.71311779731496 39.0 34.0 41.0 22.0 41.0 18 34.66363048328119 39.0 34.0 41.0 22.0 41.0 19 34.68057741937613 39.0 34.0 41.0 21.0 41.0 20 34.6024055977981 39.0 34.0 41.0 19.0 41.0 21 34.52125287117342 39.0 34.0 41.0 18.0 41.0 22 34.400003135189365 39.0 34.0 41.0 18.0 41.0 23 34.273897980938045 39.0 34.0 41.0 18.0 41.0 24 34.14600700632318 39.0 34.0 41.0 16.0 41.0 25 34.01153914696447 39.0 34.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5691828324439179 1101 2 2.8888251073576683 1101 3 1.458602131391622 1101 4 0.652334908088612 1101 5 0.3786904663893793 1101 6 0.0735283552031305 1101 7 -0.12370008787141984 1101 8 -0.09241574866154423 1101 9 -0.20995041579849527 1101 10 -0.24743846317374363 1101 11 -0.11551414712077701 1101 12 -0.32552576008166767 1101 13 -0.19797081363574875 1101 14 -0.1662321541773224 1101 15 -0.15053644514829045 1101 16 -0.11232980852737029 1101 17 -0.33283411424717 1101 18 -0.20053264627718193 1101 19 -0.31826473965379876 1101 20 -0.2909760790812683 1101 21 -0.2947407063825196 1101 22 -0.4048352164769611 1101 23 -0.19787344750857017 1101 24 -0.3435319505402319 1101 25 -0.44854912991586815 1102 1 1.3026341531796888 1102 2 3.7783759346745462 1102 3 2.4505987337740756 1102 4 1.5090665783578459 1102 5 1.2647353930247505 1102 6 1.0232832943566308 1102 7 0.9280907240984746 1102 8 0.9465654306804296 1102 9 1.0231962883663641 1102 10 0.9573020900594074 1102 11 0.9333556429710583 1102 12 0.8431169801336083 1102 13 0.9436382362865245 1102 14 0.8248532212672828 1102 15 0.9834045476619337 1102 16 0.7875547519086652 1102 17 0.7265546892766395 1102 18 0.7220104412309496 1102 19 0.725412330811551 1102 20 1.02960832012905 1102 21 1.1123759685084238 1102 22 1.159003499352501 1102 23 0.9754190979281958 1102 24 0.9307726487953119 1102 25 0.8838549917374223 1103 1 0.8133162784185757 1103 2 2.620776610822361 1103 3 1.654429371776935 1103 4 0.9277676000101707 1103 5 0.7176577959849766 1103 6 0.5125598066754975 1103 7 0.48372815044678674 1103 8 0.31453010935099 1103 9 0.4966659420602326 1103 10 0.23796675888350904 1103 11 0.4994017631922745 1103 12 0.440059563573179 1103 13 0.47241771114456554 1103 14 0.6576514051266358 1103 15 0.2576214340070919 1103 16 0.3067932290826434 1103 17 0.5221753583452582 1103 18 0.4788882283915328 1103 19 0.4437835144399287 1103 20 0.7003094720913694 1103 21 0.6583376947058923 1103 22 0.7414922884834709 1103 23 0.6259161610117729 1103 24 0.5659743931922563 1103 25 0.6413005058707526 1104 1 1.1522204598780696 1104 2 3.7263640021028195 1104 3 2.2207665933423577 1104 4 1.643720459110348 1104 5 1.1568007306105699 1104 6 0.6954895756680699 1104 7 0.6246993990354497 1104 8 0.5071939884961552 1104 9 0.6748754173881792 1104 10 0.7952235612217677 1104 11 0.7537860831179728 1104 12 0.7504457694184694 1104 13 0.6786903435090039 1104 14 0.8402724079608319 1104 15 0.6341201108501622 1104 16 0.8172425352450219 1104 17 0.7238663050449858 1104 18 0.813217814109592 1104 19 0.6989587153063184 1104 20 0.6353858039777691 1104 21 0.7218203359150053 1104 22 0.8296659419741061 1104 23 0.9369386874704801 1104 24 0.9860514606581887 1104 25 0.9062424305367713 1105 1 1.13606104189833 1105 2 3.5588818336880905 1105 3 2.053757752634496 1105 4 1.4537199054125693 1105 5 1.068414960979048 1105 6 0.5928684039429015 1105 7 0.3031622333304824 1105 8 0.4276663765946722 1105 9 0.46277527527242057 1105 10 0.36964121377808823 1105 11 0.12351458664066683 1105 12 0.1927512998525529 1105 13 0.3291337211996819 1105 14 0.35796489763772144 1105 15 0.4636714646412301 1105 16 0.35058873226411436 1105 17 0.47764764140296023 1105 18 0.26647783561991645 1105 19 0.5271144151215807 1105 20 0.6648393378279991 1105 21 0.5226584330067539 1105 22 0.5940026036463095 1105 23 0.5708840526006114 1105 24 0.5928379497612539 1105 25 0.566254209198604 1106 1 0.4221993746759338 1106 2 2.98527016838149 1106 3 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0.3595400827134654 1107 15 0.3835625382360561 1107 16 0.5883239002838607 1107 17 0.5346919247917654 1107 18 0.3226197451660866 1107 19 0.48814838973977004 1107 20 0.6028710417690206 1107 21 0.36699003481914616 1107 22 0.5645788981396578 1107 23 0.6089932278471153 1107 24 0.7036194865120038 1107 25 0.8715010351755623 1108 1 0.6460224287984175 1108 2 2.4163566796717717 1108 3 1.436267366265632 1108 4 0.9671441024266016 1108 5 0.7442273028992332 1108 6 0.6342415079762631 1108 7 0.6381970802656198 1108 8 0.620769775657493 1108 9 0.5613907538132068 1108 10 0.3710709292146035 1108 11 0.4382641565050065 1108 12 0.5437107600620266 1108 13 0.6153757230550099 1108 14 0.5169203617481557 1108 15 0.54225126372625 1108 16 0.405708892742382 1108 17 0.4036059519410884 1108 18 0.46547230926865524 1108 19 0.5679164892872777 1108 20 0.624569759717744 1108 21 0.7848030061615319 1108 22 0.7736786341879238 1108 23 0.8034719231714007 1108 24 0.8713089661500177 1108 25 0.7879933051604766 1109 1 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-1.848486754187057 2315 22 -2.1480497608738034 2315 23 -1.8550756440036196 2315 24 -1.8217919779313192 2315 25 -1.9317597812592737 2316 1 -1.9703064013948328 2316 2 -2.2823994036060284 2316 3 -1.5341496285446503 2316 4 -2.0010754407974893 2316 5 -2.164537867432543 2316 6 -2.2667237390906365 2316 7 -2.0903665594024012 2316 8 -2.24397568586388 2316 9 -2.508889733252886 2316 10 -2.6777899580857323 2316 11 -3.0053924740153803 2316 12 -2.8581110932931146 2316 13 -2.8891153009550052 2316 14 -3.3053445359720044 2316 15 -3.488653604915335 2316 16 -3.2505953014378655 2316 17 -3.2461644484950902 2316 18 -3.07114972986626 2316 19 -3.35367556256951 2316 20 -3.291145997233855 2316 21 -3.411344486821246 2316 22 -3.1576547395323153 2316 23 -3.153604064793303 2316 24 -3.140326501631332 2316 25 -3.489339543529976 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 116495.0 3 3.0 4 1068.0 5 4908.0 6 5748.0 7 5110.0 8 4732.0 9 4653.0 10 4472.0 11 4505.0 12 4246.0 13 4216.0 14 4334.0 15 4322.0 16 4716.0 17 5097.0 18 5899.0 19 7291.0 20 9377.0 21 12335.0 22 15074.0 23 18056.0 24 20853.0 25 23567.0 26 26499.0 27 30242.0 28 34992.0 29 41760.0 30 51906.0 31 64336.0 32 81879.0 33 106130.0 34 139576.0 35 187900.0 36 265528.0 37 365246.0 38 737025.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.32831538024897 19.356040354837432 13.11985993954035 25.195784325373253 2 15.885929464739707 21.940768544792643 39.433264472263204 22.740037518204446 3 18.13925686111441 25.223588504745685 30.308494383060737 26.328660251079167 4 12.274513880638391 17.616216519477774 38.73881232426439 31.370457275619444 5 13.089126833260728 37.88170930524203 35.96371595844389 13.065447903053345 6 30.623745924253825 37.63658699985479 18.811424960067587 12.928242115823796 7 27.17466634984753 32.024185510804855 23.052016091771943 17.749132047575674 8 24.62171340314378 36.83117114560198 21.264368290260087 17.282747160994155 9 26.150366982165725 15.671079033173605 20.857466040948875 37.3210879437118 10 15.12138958193075 29.09142212189616 34.581922498118885 21.205265798054203 11 33.333374585824984 22.833089118582762 25.25650799308278 18.577028302509472 12 22.838699457447227 25.078874764035746 32.101410175174586 19.981015603342442 13 28.481380275368632 22.333108919778756 26.14351906855174 23.041991736300872 14 20.86480216097409 22.399851160764523 28.109782962032792 28.62556371622859 15 22.672039391179226 30.84378671471757 23.92388750280517 22.560286391298035 16 21.962249019840797 28.34050301638219 27.891593402241494 21.80565456153552 17 21.44808681177924 28.70097912829324 28.130828205990277 21.720105853937245 18 21.20670963526197 27.816720129895845 29.961519675788416 21.015050559053766 19 23.10187455988621 27.186419310512118 27.960895825194537 21.75081030440714 20 23.159380699306006 27.598945418258158 28.44903796849043 20.792635913945404 21 22.724797573689447 27.068692112345573 27.62485912250855 22.58165119145643 22 22.82845450645193 28.104187980594702 27.82461799940596 21.242739513547406 23 22.176913011265288 27.7975515699951 28.049811805201468 21.975723613538143 24 22.64184412523148 27.084217686906452 28.71419218864669 21.559745999215373 25 22.59127633199194 27.470581804756 28.34051470755065 21.597627155701407 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1063.0 1 1063.0 2 1004.0 3 945.0 4 945.0 5 945.0 6 1983.0 7 3021.0 8 3021.0 9 3021.0 10 4534.0 11 6047.0 12 6047.0 13 6047.0 14 9728.5 15 13410.0 16 13410.0 17 13410.0 18 23083.5 19 32757.0 20 32757.0 21 32757.0 22 50757.0 23 68757.0 24 68757.0 25 68757.0 26 96838.0 27 124919.0 28 124919.0 29 124919.0 30 158374.0 31 191829.0 32 191829.0 33 191829.0 34 215350.0 35 238871.0 36 238871.0 37 238871.0 38 256780.0 39 274689.0 40 274689.0 41 274689.0 42 288388.5 43 302088.0 44 302088.0 45 302088.0 46 305782.5 47 309477.0 48 309477.0 49 309477.0 50 297415.0 51 285353.0 52 285353.0 53 285353.0 54 254654.0 55 223955.0 56 223955.0 57 223955.0 58 190801.5 59 157648.0 60 157648.0 61 157648.0 62 127942.0 63 98236.0 64 98236.0 65 98236.0 66 75523.5 67 52811.0 68 52811.0 69 52811.0 70 38338.0 71 23865.0 72 23865.0 73 23865.0 74 16680.0 75 9495.0 76 9495.0 77 9495.0 78 6429.0 79 3363.0 80 3363.0 81 3363.0 82 2217.0 83 1071.0 84 1071.0 85 1071.0 86 696.5 87 322.0 88 322.0 89 322.0 90 196.5 91 71.0 92 71.0 93 71.0 94 47.0 95 23.0 96 23.0 97 23.0 98 16.5 99 10.0 100 10.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.4286199886473145 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 3.300199332039655E-4 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 1.6500996660198276E-4 15 0.0 16 0.0 17 4.125249165049569E-5 18 0.0 19 2.8876744155346984E-4 20 2.8876744155346984E-4 21 1.6500996660198276E-4 22 6.60039866407931E-4 23 4.950298998059483E-4 24 3.712724248544612E-4 25 4.125249165049569E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2424096.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.43809868384092 #Duplication Level Percentage of deduplicated Percentage of total 1 84.229249058451 62.698651534787906 2 11.023262932907013 16.41101468035316 3 2.645251426876097 5.907224602721218 4 0.8963344640607017 2.668857331579127 5 0.4152786776864447 1.5456277595459267 6 0.21932007932443506 0.9795461824860069 7 0.12814085845172996 0.6676993306803375 8 0.09117381080056446 0.5429444100603407 9 0.05956321180205827 0.3990395014243318 >10 0.26408328896084216 3.285467566064699 >50 0.014408390108159533 0.7424930749306965 >100 0.011140593827447432 1.628437452479085 >500 0.0018435164507194304 0.9143949703585106 >1k 8.938261579123802E-4 1.3688686904335228 >5k 5.5864134869523765E-5 0.23973291209518371 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5765 0.23782061436510765 No Hit TATCAACGCAGAGTACTTTTTTTTT 3661 0.1510253719324647 No Hit GGTATCAACGCAGAGTACTTTTTTT 3478 0.143476165960424 No Hit GTCCTACAGTGGACATTTCTAAATT 3293 0.13584445500508233 No Hit CTTTAGGACGTGAAATATGGCGAGG 3171 0.13081165102372183 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3119 0.12866652145789606 No Hit CTGTAGGACGTGGAATATGGCAAGA 2972 0.1226024051852732 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 4.125249165049569E-5 0.0 0.0 0.0 9 0.0 4.125249165049569E-5 0.0 0.0 4.125249165049569E-5 10 0.0 4.125249165049569E-5 0.0 0.0 4.125249165049569E-5 11 0.0 4.125249165049569E-5 0.0 0.0 4.125249165049569E-5 12 0.0 4.125249165049569E-5 0.0 0.0 4.125249165049569E-5 13 0.0 4.125249165049569E-5 0.0 0.0 4.125249165049569E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATACCGA 50 0.0015537131 13.238001 6 TCCAACG 160 0.0 13.001608 18 CCAACGA 115 5.7134457E-9 12.333541 19 TAGGACC 665 0.0 12.228443 4 CGTTAGG 65 5.705512E-4 12.17707 1 GGTATCA 1465 0.0 12.156289 1 TACACCG 110 4.0738087E-8 12.034546 5 GTATTAG 200 0.0 11.37795 1 CCCGTTT 135 5.0913513E-9 11.206773 12 TTAGGAC 1335 0.0 10.766057 3 GGCGAGG 980 0.0 10.710044 19 AGGACGT 2050 0.0 10.701101 5 CTAGTAC 115 9.3693416E-7 10.689069 3 GGACGTG 1995 0.0 10.474751 6 GACGTGA 1125 0.0 10.422298 7 TAGGACG 2235 0.0 10.238402 4 TCCCGTT 150 2.6304406E-8 10.086096 11 GGTCTAG 130 2.525352E-6 9.893868 1 GACCTAC 160 3.543755E-8 9.893868 1 GTCCTAA 1305 0.0 9.70433 1 >>END_MODULE