FastQCFastQC Report
Thu 26 May 2016
SRR1512117_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512117_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2424096
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT72690.2998643618074532No Hit
TATCAACGCAGAGTACTTTTTTTTT49210.20300351141208928No Hit
GGTATCAACGCAGAGTACTTTTTTT46770.19293790344936834No Hit
CTTTAGGACGTGAAATATGGCGAGG35470.14632258788430821No Hit
GTCCTACAGTGGACATTTCTAAATT34880.143888690876929No Hit
GTCCTAAAGTGTGTATTTCTCATTT33470.13807208955420908No Hit
CTGTAGGACGTGGAATATGGCAAGA32260.1330805380644991No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6700.012.4800934
GGTATCA15500.011.5091741
GGCGAGG9950.010.69007819
AGGACGT21300.010.3940845
GTATTAG2053.6379788E-1210.2377161
TGTAGGA21800.010.1992752
GTAGGAC21450.010.1442073
TACTAGC856.583736E-410.0608232
CTAGGAC3700.010.0155043
GGACGTG21550.09.915246
TTAGGAC14800.09.88713
CTGTAGG22300.09.7963631
TAGGACG23500.09.6636244
GTATCAA31250.09.6465341
TCCAACG1606.6733264E-89.49679218
GGACCGC800.004530369.4965966
GACGTGA13000.09.4235457
GTTCTAG3150.09.3882661
GTCCTAC18400.09.2804591
GACGTGG10250.09.2649727
Produced by FastQC (version 0.11.5)