FastQCFastQC Report
Thu 26 May 2016
SRR1512116_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512116_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2804373
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT53380.19034557813814354No Hit
TATCAACGCAGAGTACTTTTTTTTT35520.12665932812789168No Hit
GGTATCAACGCAGAGTACTTTTTTT33980.12116790455477927No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGAC402.8615617E-416.54807311
TTCTACG550.002269403512.5877392
GGTATCA14150.011.7424071
GTGCCGA2150.010.99539711
CGTGCCG1855.456968E-1210.73388510
GTCCTAA7550.010.6106591
TATCGCA750.001895446910.5497252
CTTATAC2650.010.3483063
GTCCTAC12250.010.1727591
GTCCTAG3300.09.8901821
GTATCAA27950.09.8724891
AACGACT2400.09.85004319
TAGGACT3900.09.6986764
GCACCGT2650.09.6346296
AGTACCG3350.09.5973475
GTACCGA2700.09.456216
ACCGATC2900.09.456218
ATAGGAC3900.09.4562093
TGCGATA1607.132985E-89.45604113
CAACGAC2750.09.28411418
Produced by FastQC (version 0.11.5)