FastQCFastQC Report
Thu 26 May 2016
SRR1512116_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512116_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2804373
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT71570.2552085617712052No Hit
TATCAACGCAGAGTACTTTTTTTTT49640.17700926374629908No Hit
GGTATCAACGCAGAGTACTTTTTTT48630.17340774568860845No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG551.9585859E-413.8202195
GGTATCA18250.013.0680671
CGTGCCG957.4291165E-812.99542510
GCACCGT1657.2759576E-1211.5113046
TAGCACG600.005871210211.0849684
CTAGTAC951.3589437E-511.0016213
TATTGCG700.001490216810.8587445
ATCGGGC803.7843792E-410.6839298
GTGCCGA1409.7476004E-810.1749911
GTATAAG3200.010.135921
TTATACT2250.010.1348284
GTCCTAG3400.010.1008471
GTCTTAG2800.09.8803931
GTATTAG2450.09.7343771
AGCACCG2002.6375346E-109.50145
TTTATAC3500.09.50143
GTCCTAA9250.09.2818591
TGTAGGA14650.09.2744052
GGCGAGG6800.09.21819
GTCCTAC13700.09.1218931
Produced by FastQC (version 0.11.5)