FastQCFastQC Report
Thu 26 May 2016
SRR1512115_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512115_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2767010
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT55620.20101119981496274No Hit
TATCAACGCAGAGTACTTTTTTTTT35550.12847803224419138No Hit
GGTATCAACGCAGAGTACTTTTTTT35370.1278275105619423No Hit
GTCCTAAAGTGTGTATTTCTCATTT29600.10697467663651379No Hit
GTCCTACAGTGGACATTTCTAAATT29360.10610731439351503No Hit
CTGTAGGACGTGGAATATGGCAAGA29050.10498697149630828No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGA450.00883597313.1836882
TACGACT550.003177379312.0352544
GTATACG802.6051997E-411.1237361
CGTCTAT1001.5423562E-510.8765421
GGTATCA14950.010.5822241
GTCCTAA11400.010.40817451
TAGGACC5400.010.3318514
TACACCG1201.6177946E-610.2442935
TTAGTAC1856.548362E-1110.2229953
TCGAACT1804.292815E-109.98179919
GTAGGAC17100.09.8433693
ACCGTGC1356.865739E-79.806688
AGGACGT17500.09.726455
TAGGACA5950.09.6946644
TCCAACG2054.5474735E-119.68708618
GGACGTG17450.09.6459386
GTATTAA3000.09.5581731
GTATCAA28200.09.5371361
GTATAAC2301.8189894E-119.4578631
CCGTCCA1201.796438E-59.4564419
Produced by FastQC (version 0.11.5)