FastQCFastQC Report
Thu 26 May 2016
SRR1512115_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512115_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2767010
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT68950.2491859443948522No Hit
TATCAACGCAGAGTACTTTTTTTTT47600.1720268448614208No Hit
GGTATCAACGCAGAGTACTTTTTTT46240.1671117921510945No Hit
GTCCTACAGTGGACATTTCTAAATT32140.11615426037491733No Hit
CTGTAGGACGTGGAATATGGCAAGA31720.1146363764496695No Hit
CTTTAGGACGTGAAATATGGCGAGG31050.11221499018796463No Hit
GTCCTAAAGTGTGTATTTCTCATTT30810.11134762794496587No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCGA456.777288E-414.77415219
TACGCCC400.00527578414.2519114
TAGGACC5300.013.44524
GGTATCA18100.012.7506171
TATTCCG550.003066403612.0925315
AATACCG600.00587175611.0848215
GGACGTG17600.010.0909096
AGGACGT17950.09.9511965
GTCGAGG1252.5797872E-69.87757519
TCCAACG1651.0275471E-89.78529918
CTAGGAC3600.09.7651993
GTCCTAA10650.09.7604831
TAGACTG3800.09.7513085
TCTACAC4350.09.6104843
TGTAGGA20050.09.5723562
GACGTGG8950.09.5503837
GGACGGT900.00111696459.4973256
TTAGGAC11450.09.45978453
TGGCGAG17450.09.36142918
GTAGGAC18900.09.3001893
Produced by FastQC (version 0.11.5)