##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512115_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2767010 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.642191029306 31.0 31.0 34.0 27.0 34.0 2 30.83820152438914 31.0 31.0 34.0 27.0 34.0 3 30.936156717901273 31.0 31.0 34.0 27.0 34.0 4 34.54370746762751 37.0 35.0 37.0 31.0 37.0 5 34.12725830408998 37.0 35.0 37.0 30.0 37.0 6 34.06137238390898 37.0 35.0 37.0 30.0 37.0 7 33.870154065218415 37.0 35.0 37.0 29.0 37.0 8 33.76554078228846 37.0 35.0 37.0 29.0 37.0 9 35.22630095301427 39.0 35.0 39.0 29.0 39.0 10 35.02192872450768 38.0 35.0 39.0 28.0 39.0 11 35.01429232275995 38.0 35.0 39.0 28.0 39.0 12 34.8249124506236 38.0 35.0 39.0 27.0 39.0 13 34.794022067141064 38.0 35.0 39.0 27.0 39.0 14 35.757714283649136 39.0 36.0 41.0 27.0 41.0 15 35.69076476051767 39.0 36.0 41.0 27.0 41.0 16 35.57268929277451 39.0 35.0 41.0 27.0 41.0 17 35.50193204939628 39.0 35.0 41.0 27.0 41.0 18 35.418226533333815 39.0 35.0 41.0 27.0 41.0 19 35.431533315745156 39.0 35.0 41.0 27.0 41.0 20 35.31529954716463 39.0 35.0 40.0 27.0 41.0 21 35.183114264133486 39.0 34.0 40.0 26.0 41.0 22 35.060683192326735 39.0 34.0 40.0 26.0 41.0 23 35.12322290125442 39.0 35.0 40.0 26.0 41.0 24 35.00069678100188 39.0 35.0 41.0 25.0 41.0 25 34.55151914882852 38.0 34.0 40.0 24.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13770777485041563 1101 2 0.019432585216200948 1101 3 0.0347522844703434 1101 4 0.13685675250478369 1101 5 0.026955030227085786 1101 6 -0.0759364883171827 1101 7 -0.07661345265492514 1101 8 -0.027699290188486714 1101 9 -0.1116091634166807 1101 10 0.05491468889390205 1101 11 -0.015436971250920806 1101 12 -0.18179651954645948 1101 13 0.012925221325986058 1101 14 -0.03201202987183649 1101 15 -0.009086945306236771 1101 16 -0.05647568387836088 1101 17 -0.016243691844138652 1101 18 -0.07100291957987537 1101 19 -0.28515652519596557 1101 20 -0.15682422325106415 1101 21 -0.2685434607302213 1101 22 -0.3848689336456701 1101 23 -0.4290808287864323 1101 24 -0.6652328034939003 1101 25 -0.5912686016775623 1102 1 0.22821663382668333 1102 2 0.22254020002751673 1102 3 0.307321713045809 1102 4 0.3441212321467475 1102 5 0.17184604045810659 1102 6 0.1571419626561834 1102 7 0.2980223456030018 1102 8 0.3182183403196177 1102 9 0.29777506107701157 1102 10 0.42339226121282536 1102 11 0.42957344757104465 1102 12 0.5232026314919693 1102 13 0.5485575093424941 1102 14 0.6818980322754769 1102 15 0.6008055204853235 1102 16 0.395229066954883 1102 17 0.686730692783371 1102 18 0.43183272284582586 1102 19 0.23226934577905212 1102 20 0.5627925779337701 1102 21 0.6402840222469237 1102 22 0.6943323943351913 1102 23 0.8464188328753792 1102 24 0.7136389390488773 1102 25 0.48371617102648656 1103 1 0.09600458614436036 1103 2 0.28967852241600056 1103 3 0.2785012054100875 1103 4 0.21883517351314907 1103 5 0.24748146775669255 1103 6 0.36737660295249697 1103 7 0.3745452367129545 1103 8 0.49276826938670837 1103 9 0.48973645238917385 1103 10 0.4922766245177215 1103 11 0.36943975026520803 1103 12 0.45476962297360046 1103 13 0.3408765341272897 1103 14 0.5040794921454221 1103 15 0.43886223150912684 1103 16 0.5687968480064853 1103 17 0.5972753837277125 1103 18 0.5872592511130748 1103 19 0.5454365484546031 1103 20 0.5420222336684262 1103 21 0.7255668070538022 1103 22 0.7146184850651238 1103 23 0.5320536342953233 1103 24 0.676221426643643 1103 25 0.6656265933501331 1104 1 0.16231115512362493 1104 2 0.14492506680728212 1104 3 0.15405568108792167 1104 4 0.1346088643690777 1104 5 0.16851956252011036 1104 6 0.21286686743947314 1104 7 0.11921437879163932 1104 8 0.19785781617033393 1104 9 0.35117568019352774 1104 10 0.22403292103552985 1104 11 0.41777881525723615 1104 12 0.5522846581942318 1104 13 0.41268424943829274 1104 14 0.4589509866961876 1104 15 0.5188479438961551 1104 16 0.7058540981393975 1104 17 0.6204159173534407 1104 18 0.6860348359083943 1104 19 0.697410648002716 1104 20 0.6046136079374307 1104 21 0.6353949403605554 1104 22 0.6484905112480064 1104 23 0.5875925763474186 1104 24 0.5099140269892075 1104 25 0.4790611029195091 1105 1 0.01866148005699486 1105 2 0.07025762677645275 1105 3 -0.10273186888639074 1105 4 0.13963514603954508 1105 5 0.1359312695542485 1105 6 0.052560970470850066 1105 7 0.14094354512380391 1105 8 0.02394345287114419 1105 9 -0.02564140780942381 1105 10 -0.021347340316062002 1105 11 0.06368059566730722 1105 12 0.01586650668175338 1105 13 0.0647373606903443 1105 14 0.23527969623506095 1105 15 0.3220429882107254 1105 16 0.17697662350788335 1105 17 0.23689281376813653 1105 18 -0.019927059181682694 1105 19 0.08672545304269619 1105 20 0.07308212612888099 1105 21 -0.032658103656977744 1105 22 0.1791162086806537 1105 23 0.12746063613997904 1105 24 0.10482194079255436 1105 25 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1107 11 -0.024498772641145194 1107 12 0.16717478025104526 1107 13 0.00963883659790099 1107 14 0.03964295807460161 1107 15 0.272617783589844 1107 16 0.11278999745940155 1107 17 0.12748976609513818 1107 18 0.21671139130860695 1107 19 0.3337093684517001 1107 20 0.12460770965012102 1107 21 -0.019541970803693687 1107 22 0.13769150680741404 1107 23 0.13044452734992973 1107 24 0.12842255964625338 1107 25 -0.11799824338343967 1108 1 -0.044089968423879355 1108 2 -0.07647360031507233 1108 3 -0.17110291853613901 1108 4 -0.019179370422449438 1108 5 0.04461160370261297 1108 6 0.05893979122621573 1108 7 0.05169739741774748 1108 8 -0.08976286906548125 1108 9 -0.04719773915028469 1108 10 -0.06757945507519736 1108 11 0.044693224860793634 1108 12 0.07026195228171872 1108 13 -0.08513166819670204 1108 14 -0.057077187432312826 1108 15 0.11711563015726512 1108 16 0.14313515589742565 1108 17 0.10189393768836652 1108 18 0.06166496236088648 1108 19 0.08910632896648707 1108 20 0.17047784238719288 1108 21 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-0.6065545733442335 2315 3 -0.7188432298969296 2315 4 -0.71103507502486 2315 5 -0.9052848882374605 2315 6 -0.8484494204393158 2315 7 -0.8626452329507899 2315 8 -0.9961526004963304 2315 9 -1.1497121038973788 2315 10 -1.1756178838887124 2315 11 -1.1171110995154905 2315 12 -1.2513079080936507 2315 13 -1.2301898035920047 2315 14 -1.3903032746674455 2315 15 -1.3795599633008138 2315 16 -1.376058780648485 2315 17 -1.4157168951465593 2315 18 -1.4858401640916057 2315 19 -1.5768850432851664 2315 20 -1.456258787895905 2315 21 -1.3122849838818738 2315 22 -1.3119143182974469 2315 23 -1.3741735221374256 2315 24 -1.4609370620259483 2315 25 -1.3337052323077643 2316 1 -1.2966159560008883 2316 2 -1.2862004050514884 2316 3 -1.3784798457973864 2316 4 -1.3205523570000963 2316 5 -1.507297609059087 2316 6 -1.59028975241975 2316 7 -1.7373282490359685 2316 8 -1.6738469332833006 2316 9 -1.8748763786007814 2316 10 -2.160770454883348 2316 11 -1.9210482653561058 2316 12 -1.9904173408194765 2316 13 -2.0366355022859395 2316 14 -2.3158558572072536 2316 15 -2.3082499650244586 2316 16 -2.427928284499039 2316 17 -2.3677360593090597 2316 18 -2.318809255568347 2316 19 -2.213149608894227 2316 20 -2.28547478575598 2316 21 -2.258785741635016 2316 22 -2.4181361464663524 2316 23 -2.333776417209755 2316 24 -2.1055854913955443 2316 25 -2.3923114827955914 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 46633.0 3 1.0 4 853.0 5 7377.0 6 9557.0 7 7927.0 8 6219.0 9 5767.0 10 5450.0 11 5020.0 12 4907.0 13 4725.0 14 4629.0 15 4661.0 16 4926.0 17 5169.0 18 5636.0 19 6212.0 20 7603.0 21 9915.0 22 12484.0 23 15708.0 24 19827.0 25 23359.0 26 26728.0 27 30859.0 28 35850.0 29 43972.0 30 55549.0 31 71750.0 32 92429.0 33 119107.0 34 153695.0 35 203808.0 36 291424.0 37 492779.0 38 924495.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.84170200039345 19.688054187817905 13.625752134723307 24.844491677065346 2 15.399546803228034 21.02529445141145 39.60708490392156 23.96807384143895 3 18.44521703933126 25.123544909487133 30.537547750098483 25.893690301083115 4 13.083798034701719 17.703152500352363 37.676914792501655 31.53613467244426 5 13.397971607421747 37.88672403424987 35.55411234591517 13.161192012413208 6 31.77639401375492 37.417067520536605 17.90373001904583 12.902808446662641 7 27.820318683344112 31.951312066093003 22.37205503413432 17.856314216428565 8 25.505727701430914 36.464691221668694 20.498000653437924 17.53158042346247 9 26.46759498520063 15.272369814348341 20.11879248719737 38.14124271325366 10 15.167202142384738 28.924904499803038 34.2573752895725 21.650518068239723 11 34.341726267704125 22.481125836191413 24.4773238983596 18.699823997744858 12 23.134683286290976 24.79203183219432 31.452506496181798 20.620778385332905 13 28.833433923260127 21.79735526796072 25.95465863874724 23.41455217003191 14 21.32753405300306 21.7680095120726 27.562748237266945 29.3417081976574 15 23.179171741338124 30.211491826917865 23.559763065547287 23.049573366196725 16 22.703568111427135 27.94283360016769 27.030766061561035 22.322832226844138 17 22.028254325065685 28.303584013068257 27.56108579296786 22.107075868898196 18 21.95116750571917 27.485010896238176 29.02194065073852 21.54188094730413 19 23.496354184736727 27.024786339613037 27.535771490360705 21.943087985289527 20 23.678627832931575 27.151365553431322 27.776950571194174 21.393056042442925 21 23.335188524797527 26.662245528566935 27.234523908478824 22.76804203815671 22 23.418780560966532 27.356749704554733 27.307563037358012 21.91690669712072 23 22.81668660395156 27.184722859693316 27.490287349883086 22.50830318647204 24 23.110098653204496 26.7319969467238 27.92356256035714 22.234341839714563 25 23.041880693709636 26.995976478318052 27.746650769666125 22.21549205830619 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 762.0 1 762.0 2 791.0 3 820.0 4 820.0 5 820.0 6 1918.5 7 3017.0 8 3017.0 9 3017.0 10 4458.0 11 5899.0 12 5899.0 13 5899.0 14 9579.5 15 13260.0 16 13260.0 17 13260.0 18 23370.0 19 33480.0 20 33480.0 21 33480.0 22 51377.5 23 69275.0 24 69275.0 25 69275.0 26 98436.5 27 127598.0 28 127598.0 29 127598.0 30 162009.5 31 196421.0 32 196421.0 33 196421.0 34 224041.5 35 251662.0 36 251662.0 37 251662.0 38 275733.5 39 299805.0 40 299805.0 41 299805.0 42 321224.5 43 342644.0 44 342644.0 45 342644.0 46 350362.5 47 358081.0 48 358081.0 49 358081.0 50 350765.0 51 343449.0 52 343449.0 53 343449.0 54 310616.5 55 277784.0 56 277784.0 57 277784.0 58 238778.5 59 199773.0 60 199773.0 61 199773.0 62 162560.5 63 125348.0 64 125348.0 65 125348.0 66 96741.0 67 68134.0 68 68134.0 69 68134.0 70 49813.0 71 31492.0 72 31492.0 73 31492.0 74 21865.0 75 12238.0 76 12238.0 77 12238.0 78 8307.0 79 4376.0 80 4376.0 81 4376.0 82 2821.0 83 1266.0 84 1266.0 85 1266.0 86 804.0 87 342.0 88 342.0 89 342.0 90 204.0 91 66.0 92 66.0 93 66.0 94 40.5 95 15.0 96 15.0 97 15.0 98 9.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4283323876675545 2 0.0 3 0.0 4 0.0 5 0.056703806636043956 6 0.0 7 0.0 8 0.0038308499065778587 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 7.228018691656337E-5 20 0.0 21 0.0 22 0.0 23 0.0 24 0.005276453644909126 25 0.0011203428972067322 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2767010.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.42395785233941 #Duplication Level Percentage of deduplicated Percentage of total 1 78.97491845348037 53.24801573199402 2 13.437505306569514 18.1201958286146 3 3.966453836312636 8.023020488483795 4 1.5439246473668746 4.163900414050086 5 0.7485258581746527 2.5234287956476984 6 0.38539403089633106 1.5590874537418453 7 0.22739780424753595 1.0732441978510274 8 0.13930780905018836 0.7514147076721317 9 0.11692291128588526 0.7095064898261112 >10 0.4253192337530791 4.757277467246328 >50 0.019329784967936 0.8979902201607111 >100 0.01183862630132259 1.5031105062760348 >500 0.001982759508048745 0.893091358977788 >1k 0.0011253499908023827 1.5275916158118883 >5k 5.358809480011347E-5 0.24912472364585184 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6895 0.2491859443948522 No Hit TATCAACGCAGAGTACTTTTTTTTT 4760 0.1720268448614208 No Hit GGTATCAACGCAGAGTACTTTTTTT 4624 0.1671117921510945 No Hit GTCCTACAGTGGACATTTCTAAATT 3214 0.11615426037491733 No Hit CTGTAGGACGTGGAATATGGCAAGA 3172 0.1146363764496695 No Hit CTTTAGGACGTGAAATATGGCGAGG 3105 0.11221499018796463 No Hit GTCCTAAAGTGTGTATTTCTCATTT 3081 0.11134762794496587 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 7.228018691656337E-5 0.0 4 0.0 0.0 0.0 7.228018691656337E-5 0.0 5 3.6140093458281685E-5 0.0 0.0 1.0842028037484505E-4 0.0 6 3.6140093458281685E-5 0.0 0.0 1.0842028037484505E-4 0.0 7 3.6140093458281685E-5 0.0 0.0 1.4456037383312674E-4 0.0 8 3.6140093458281685E-5 0.0 0.0 1.4456037383312674E-4 0.0 9 3.6140093458281685E-5 0.0 0.0 2.168405607496901E-4 0.0 10 7.228018691656337E-5 0.0 0.0 2.529806542079718E-4 0.0 11 7.228018691656337E-5 0.0 0.0 2.891207476662535E-4 0.0 12 7.228018691656337E-5 0.0 0.0 2.891207476662535E-4 0.0 13 7.228018691656337E-5 0.0 0.0 3.6140093458281685E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCCGA 45 6.777288E-4 14.774152 19 TACGCCC 40 0.005275784 14.251911 4 TAGGACC 530 0.0 13.4452 4 GGTATCA 1810 0.0 12.750617 1 TATTCCG 55 0.0030664036 12.092531 5 AATACCG 60 0.005871756 11.084821 5 GGACGTG 1760 0.0 10.090909 6 AGGACGT 1795 0.0 9.951196 5 GTCGAGG 125 2.5797872E-6 9.877575 19 TCCAACG 165 1.0275471E-8 9.785299 18 CTAGGAC 360 0.0 9.765199 3 GTCCTAA 1065 0.0 9.760483 1 TAGACTG 380 0.0 9.751308 5 TCTACAC 435 0.0 9.610484 3 TGTAGGA 2005 0.0 9.572356 2 GACGTGG 895 0.0 9.550383 7 GGACGGT 90 0.0011169645 9.497325 6 TTAGGAC 1145 0.0 9.4597845 3 TGGCGAG 1745 0.0 9.361429 18 GTAGGAC 1890 0.0 9.300189 3 >>END_MODULE