FastQCFastQC Report
Thu 26 May 2016
SRR1512114_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512114_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1406216
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT47460.3375014933694397No Hit
TATCAACGCAGAGTACTTTTTTTTT31110.22123201556517635No Hit
GGTATCAACGCAGAGTACTTTTTTT30770.21881417932949135No Hit
GTACTTTTTTTTTTTTTTTTTTTTT14430.10261581435568931No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAACG500.001149665813.7765982
GGTATCA10100.013.4453371
TTAGACT701.1328999E-412.1647754
AGTACCG600.006055483611.0384065
GATCTAG854.7668192E-410.4192751
TAAGATA1002.5078281E-510.4076414
GTATAAA2600.010.2189051
TAATACC750.002738000810.0922584
CGTGCGC750.002738000810.09225810
TTAGCAC1054.2906824E-59.9120393
GTCCTAG1302.7032056E-69.8404271
GATATAC2500.09.8404271
GTACTAA1201.1194465E-59.8404271
GTAGTAA1201.1194465E-59.8404271
GTATCAA19550.09.7649251
TAGCACA1951.7644197E-109.7040944
TAGAACA3850.09.5843694
CTAGAAC2002.8558134E-109.4614923
AATCCCG1201.7831451E-59.46149219
GTACCGA800.00466879889.4614926
Produced by FastQC (version 0.11.5)