##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512114_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1406216 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.514654220973163 31.0 30.0 34.0 25.0 34.0 2 27.074391843073894 31.0 26.0 34.0 10.0 34.0 3 28.708884694812177 31.0 28.0 34.0 16.0 34.0 4 33.03243882874324 35.0 33.0 37.0 27.0 37.0 5 33.4177601449564 35.0 35.0 37.0 28.0 37.0 6 33.67490200651962 37.0 35.0 37.0 29.0 37.0 7 33.73039774828334 37.0 35.0 37.0 29.0 37.0 8 33.778633581185254 37.0 35.0 37.0 29.0 37.0 9 35.384880416664295 39.0 35.0 39.0 30.0 39.0 10 35.26412514151453 39.0 35.0 39.0 30.0 39.0 11 35.28545685726802 39.0 35.0 39.0 30.0 39.0 12 35.1916540559914 39.0 35.0 39.0 29.0 39.0 13 35.18027671424589 39.0 35.0 39.0 29.0 39.0 14 36.30769099484005 40.0 36.0 41.0 29.0 41.0 15 36.2338765879495 40.0 36.0 41.0 29.0 41.0 16 36.20618880740939 40.0 36.0 41.0 29.0 41.0 17 36.14479070071739 40.0 36.0 41.0 29.0 41.0 18 36.10374153046189 40.0 36.0 41.0 29.0 41.0 19 36.12405348822656 40.0 36.0 41.0 28.0 41.0 20 36.052428645385916 40.0 36.0 41.0 28.0 41.0 21 35.99316321247945 40.0 36.0 41.0 28.0 41.0 22 35.88922967737531 40.0 36.0 41.0 27.0 41.0 23 35.783780727854044 40.0 36.0 41.0 27.0 41.0 24 35.67643662140098 40.0 36.0 41.0 27.0 41.0 25 35.54824792208309 40.0 36.0 41.0 27.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.8014927120884856 1101 2 3.2627388371098114 1101 3 1.7134714363438803 1101 4 0.917688320555456 1101 5 0.5929515252885409 1101 6 0.10993958564439055 1101 7 -0.029452223788631215 1101 8 0.019662931707067344 1101 9 0.05216342864772372 1101 10 -0.22372631584040192 1101 11 -0.009131458133630588 1101 12 -0.17045797186317913 1101 13 -0.0953440746012646 1101 14 -0.04923573497025302 1101 15 0.1306113193763565 1101 16 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1.8334272814787695 1103 4 1.0385488019511797 1103 5 0.8760578021000569 1103 6 0.8248867254501775 1103 7 0.7066686097690678 1103 8 0.4050209271479872 1103 9 0.6433207886893655 1103 10 0.5999674360666916 1103 11 0.5518749497065514 1103 12 0.5772346671977218 1103 13 0.6811847427304158 1103 14 0.689089252016629 1103 15 0.3752813245816071 1103 16 0.40596380567671986 1103 17 0.5191744227420472 1103 18 0.5751685654263525 1103 19 0.6728045260170603 1103 20 0.8167361046873225 1103 21 0.8519982887403614 1103 22 0.7346053804775821 1103 23 0.6376710920280644 1103 24 0.5469160905206678 1103 25 0.4689040131752549 1104 1 1.4764099294846105 1104 2 4.068746291007866 1104 3 2.4350918971554947 1104 4 1.7372915182500108 1104 5 1.3357061424103378 1104 6 0.9984483903882833 1104 7 1.0513629943556495 1104 8 1.0894053704340863 1104 9 1.2231334052330567 1104 10 1.153766514285536 1104 11 0.9883663597307901 1104 12 1.088264335797156 1104 13 1.1911114444699038 1104 14 1.0216621965037689 1104 15 0.9182195602287067 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-1.538302505358196 2315 20 -1.6956726703296567 2315 21 -1.5494742759707378 2315 22 -1.6244355670944088 2315 23 -1.5413040607311004 2315 24 -1.4935900782378795 2315 25 -1.5925094272560898 2316 1 -1.4216923490756699 2316 2 -1.4295820823888086 2316 3 -1.010054515056634 2316 4 -1.5159738361032176 2316 5 -1.4092838699782142 2316 6 -1.6071079429014006 2316 7 -1.5864306692688217 2316 8 -1.8277087442360376 2316 9 -2.0437066710551335 2316 10 -2.11347547492754 2316 11 -2.4357812311248637 2316 12 -2.2922764553541413 2316 13 -2.322993892843421 2316 14 -2.398735712344795 2316 15 -2.9889235012037574 2316 16 -2.538004157629679 2316 17 -2.3103449488488863 2316 18 -2.109614785165604 2316 19 -2.4130161948670477 2316 20 -2.524964250430891 2316 21 -2.454479352073463 2316 22 -2.204554543623459 2316 23 -2.408688312559022 2316 24 -2.4117353573514464 2316 25 -2.75332568453549 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 45610.0 3 1.0 4 393.0 5 1878.0 6 2319.0 7 2038.0 8 1862.0 9 1868.0 10 1784.0 11 1723.0 12 1637.0 13 1700.0 14 1769.0 15 1784.0 16 1852.0 17 2011.0 18 2381.0 19 2837.0 20 3678.0 21 4713.0 22 5782.0 23 6898.0 24 7961.0 25 9328.0 26 10719.0 27 12283.0 28 14440.0 29 18185.0 30 22901.0 31 30100.0 32 40014.0 33 55751.0 34 77822.0 35 111296.0 36 166158.0 37 239657.0 38 493083.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.800394818434725 18.62160578460208 13.010519009881838 25.56748038708136 2 16.502687134152566 21.43486898649258 38.300944232166025 23.761499647188828 3 17.904432889399637 24.659938444733953 30.384805748192314 27.0508229176741 4 12.523751685374082 16.946543063085613 37.76290413421551 32.76680111732479 5 13.529429333758113 37.21490866268056 35.415327375026315 13.84033462853502 6 31.327406315957152 36.761208804337315 18.253596886964736 13.657787992740802 7 27.3937289861586 31.966568436143522 22.540136081512372 18.099566496185506 8 25.474892832659656 35.09655727585883 20.98040693721857 18.44814295426294 9 26.09471091212161 15.336904145593563 20.265805537698334 38.302579404586496 10 15.218430170045 28.386890776381442 33.92124680703391 22.473432246539648 11 33.62449296551881 22.837814389823468 24.200122882971037 19.337569761686684 12 22.424293280690875 24.846111834881697 31.402928141907076 21.326666742520352 13 28.320613618391484 21.614389254566866 26.197682290629604 23.867314836412046 14 21.668171894667225 21.086182006954864 27.767900953627123 29.477745144750784 15 23.46723405223664 28.63009665655916 24.227003532885416 23.67566575831878 16 22.895629120988524 27.730803802545275 26.30449376198251 23.069073314483692 17 21.902182879443842 27.613609857945008 27.48269113706571 23.001516125545436 18 21.789611268823567 27.290970946141986 28.48523982090945 22.434177964125 19 22.975253393333727 26.981047679121158 27.98836307159726 22.055335855947856 20 23.24368837175565 26.535505975619643 28.034424347929978 22.186381304694734 21 23.35907143710497 26.26374611012817 27.677753631021123 22.699428821745734 22 23.187110299472057 26.2812471554706 27.74276636173311 22.78887618332423 23 23.08257384398325 26.25045512470417 27.657359366466412 23.00961166484617 24 22.698126101806768 26.357813503359733 27.858012975274725 23.08604741955877 25 22.974351024309208 26.284368830961956 27.80924125454411 22.93203889018472 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 782.0 1 782.0 2 730.0 3 678.0 4 678.0 5 678.0 6 1598.0 7 2518.0 8 2518.0 9 2518.0 10 3256.5 11 3995.0 12 3995.0 13 3995.0 14 5881.0 15 7767.0 16 7767.0 17 7767.0 18 13151.0 19 18535.0 20 18535.0 21 18535.0 22 27022.5 23 35510.0 24 35510.0 25 35510.0 26 48485.0 27 61460.0 28 61460.0 29 61460.0 30 77359.5 31 93259.0 32 93259.0 33 93259.0 34 106451.0 35 119643.0 36 119643.0 37 119643.0 38 132387.5 39 145132.0 40 145132.0 41 145132.0 42 156011.5 43 166891.0 44 166891.0 45 166891.0 46 172457.0 47 178023.0 48 178023.0 49 178023.0 50 177647.0 51 177271.0 52 177271.0 53 177271.0 54 162182.0 55 147093.0 56 147093.0 57 147093.0 58 127678.0 59 108263.0 60 108263.0 61 108263.0 62 89154.5 63 70046.0 64 70046.0 65 70046.0 66 54879.5 67 39713.0 68 39713.0 69 39713.0 70 29059.0 71 18405.0 72 18405.0 73 18405.0 74 12918.0 75 7431.0 76 7431.0 77 7431.0 78 5031.0 79 2631.0 80 2631.0 81 2631.0 82 1727.5 83 824.0 84 824.0 85 824.0 86 533.0 87 242.0 88 242.0 89 242.0 90 158.0 91 74.0 92 74.0 93 74.0 94 47.5 95 21.0 96 21.0 97 21.0 98 15.0 99 9.0 100 9.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 3.8555954419520186 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 2.844513218452926E-4 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 4.2667698276793893E-4 15 0.0 16 0.0 17 0.0 18 0.0 19 2.1333849138396947E-4 20 1.422256609226463E-4 21 0.0 22 5.689026436905852E-4 23 5.689026436905852E-4 24 2.1333849138396947E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1406216.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.39746764866925 #Duplication Level Percentage of deduplicated Percentage of total 1 84.75996024853555 64.75446320989985 2 10.500882077046581 16.04481597527314 3 2.5506152164666025 5.8458163045263225 4 0.9269833354972267 2.832767175380195 5 0.4366674492114307 1.66801436621786 6 0.22833786201228423 1.0466660655629871 7 0.1385180883013707 0.7407701818831632 8 0.0982954985910292 0.6007621738894373 9 0.06590503764468796 0.45314801832099394 >10 0.2784209840022076 3.4245050586880375 >50 0.008767509621604537 0.44962733494193513 >100 0.005710588463304014 0.8851828291067642 >500 3.7444184241831245E-4 0.1941659979819549 >1k 5.616627636274686E-4 1.0592953083273358 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4746 0.3375014933694397 No Hit TATCAACGCAGAGTACTTTTTTTTT 3111 0.22123201556517635 No Hit GGTATCAACGCAGAGTACTTTTTTT 3077 0.21881417932949135 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1443 0.10261581435568931 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 7.111283046132316E-5 0.0 5 0.0 0.0 0.0 7.111283046132316E-5 0.0 6 0.0 0.0 0.0 7.111283046132316E-5 0.0 7 0.0 0.0 0.0 7.111283046132316E-5 0.0 8 0.0 0.0 0.0 7.111283046132316E-5 7.111283046132316E-5 9 0.0 0.0 0.0 7.111283046132316E-5 7.111283046132316E-5 10 0.0 0.0 0.0 7.111283046132316E-5 7.111283046132316E-5 11 0.0 0.0 0.0 7.111283046132316E-5 7.111283046132316E-5 12 0.0 0.0 0.0 7.111283046132316E-5 1.422256609226463E-4 13 0.0 0.0 0.0 7.111283046132316E-5 1.422256609226463E-4 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCAACG 50 0.0011496658 13.776598 2 GGTATCA 1010 0.0 13.445337 1 TTAGACT 70 1.1328999E-4 12.164775 4 AGTACCG 60 0.0060554836 11.038406 5 GATCTAG 85 4.7668192E-4 10.419275 1 TAAGATA 100 2.5078281E-5 10.407641 4 GTATAAA 260 0.0 10.218905 1 TAATACC 75 0.0027380008 10.092258 4 CGTGCGC 75 0.0027380008 10.092258 10 TTAGCAC 105 4.2906824E-5 9.912039 3 GTCCTAG 130 2.7032056E-6 9.840427 1 GATATAC 250 0.0 9.840427 1 GTACTAA 120 1.1194465E-5 9.840427 1 GTAGTAA 120 1.1194465E-5 9.840427 1 GTATCAA 1955 0.0 9.764925 1 TAGCACA 195 1.7644197E-10 9.704094 4 TAGAACA 385 0.0 9.584369 4 CTAGAAC 200 2.8558134E-10 9.461492 3 AATCCCG 120 1.7831451E-5 9.461492 19 GTACCGA 80 0.0046687988 9.461492 6 >>END_MODULE