FastQCFastQC Report
Thu 26 May 2016
SRR1512114_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512114_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1406216
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT63290.45007310398971423No Hit
TATCAACGCAGAGTACTTTTTTTTT49450.351652946631243No Hit
GGTATCAACGCAGAGTACTTTTTTT46600.3313857899497659No Hit
ACGCAGAGTACTTTTTTTTTTTTTT21950.15609266286260431No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17910.12736307935622976No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG1006.2755134E-1014.2971571
GGTATCA13100.013.8242241
ACTAATC655.466819E-513.1499818
TCCAACG701.0930285E-412.21156518
ACCGTGC802.8745364E-511.8715118
TAGGACC1850.011.8128014
TGTGCGG752.0792136E-411.3966510
GCCTCGA1201.2797864E-711.08007616
CCTATAC803.7643258E-410.6893013
TAATACT1909.094947E-1210.5017694
TTGGACT1553.6488927E-910.4211114
CCTCGAA1106.0639522E-610.36059217
TTAGACT1201.5195474E-610.2934014
ACTGGTC1409.7221346E-810.175588
TCGAACT1054.1135056E-59.95016519
TTAGGAC2700.09.8535123
ATTATAC1356.423088E-79.8535123
TGGTATC3650.09.631762
TAGGACG1804.1636667E-99.50164
GCGTGCG800.0045259559.4972099
Produced by FastQC (version 0.11.5)