##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512114_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1406216 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.794143289508867 31.0 28.0 31.0 23.0 34.0 2 28.99144157085398 31.0 28.0 33.0 24.0 34.0 3 29.010427274330542 31.0 28.0 34.0 24.0 34.0 4 32.59589849639031 35.0 32.0 37.0 26.0 37.0 5 31.99095302570871 35.0 32.0 37.0 22.0 37.0 6 31.926830586481735 35.0 32.0 37.0 22.0 37.0 7 31.656263333655712 35.0 32.0 37.0 19.0 37.0 8 31.48035365832845 35.0 32.0 37.0 18.0 37.0 9 32.63919269870347 37.0 32.0 39.0 17.0 39.0 10 32.316169777615954 37.0 32.0 39.0 17.0 39.0 11 32.37838354847335 37.0 32.0 39.0 17.0 39.0 12 32.04072418462029 37.0 32.0 39.0 11.0 39.0 13 32.00107807050979 37.0 32.0 39.0 11.0 39.0 14 32.59373524408768 37.0 31.0 40.0 10.0 41.0 15 32.46796580326209 37.0 31.0 40.0 10.0 41.0 16 32.3010689680675 37.0 31.0 40.0 10.0 41.0 17 32.191943485211375 37.0 31.0 40.0 10.0 41.0 18 32.07148546169294 37.0 31.0 40.0 8.0 41.0 19 32.069126649106536 37.0 31.0 40.0 2.0 41.0 20 31.895531696410792 37.0 31.0 40.0 2.0 41.0 21 31.717268897523567 37.0 31.0 40.0 2.0 41.0 22 31.504969364592636 37.0 30.0 40.0 2.0 41.0 23 31.524696774890913 37.0 31.0 40.0 2.0 41.0 24 31.27703923152631 37.0 30.0 40.0 2.0 41.0 25 30.70689495781587 36.0 30.0 39.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1730971191985411 1101 2 0.04327327404295289 1101 3 0.13534189248580475 1101 4 0.10274889831845258 1101 5 0.3643892409556919 1101 6 0.032889678637712194 1101 7 0.12730165528425985 1101 8 0.026603351983538914 1101 9 0.22457387149205488 1101 10 0.10387982363982218 1101 11 -0.0790016007991241 1101 12 -0.07618581835658489 1101 13 0.23040591482633133 1101 14 0.2012759097073058 1101 15 0.3562878959315796 1101 16 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-1.3825656954231675 2315 14 -1.3673335896063001 2315 15 -1.5545734928850088 2315 16 -1.3142899684940232 2315 17 -1.3818811312495853 2315 18 -1.3811407363261665 2315 19 -1.2802167823925608 2315 20 -1.1539034241273 2315 21 -1.251534432887162 2315 22 -1.2719667331869786 2315 23 -1.4439361498957908 2315 24 -1.1995355253870414 2315 25 -1.538702000221253 2316 1 -1.361384079045724 2316 2 -1.5718586323781523 2316 3 -1.7480766634066178 2316 4 -1.6161433592497652 2316 5 -2.121661409903325 2316 6 -2.0919545827919137 2316 7 -2.290252683524436 2316 8 -2.176432984502277 2316 9 -2.4169906035218993 2316 10 -2.6854258764672707 2316 11 -2.521893436025451 2316 12 -2.5079323528905775 2316 13 -2.4694266567832415 2316 14 -2.6895366459136163 2316 15 -2.7481723360547825 2316 16 -2.9540530134681298 2316 17 -2.8970980729572986 2316 18 -2.80970907575027 2316 19 -2.6009067109722643 2316 20 -3.113765340825985 2316 21 -2.8129879891808933 2316 22 -3.0522222183442977 2316 23 -2.997849572819149 2316 24 -2.9053705207212417 2316 25 -2.558476899600201 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 59662.0 3 4.0 4 1032.0 5 7786.0 6 9420.0 7 7631.0 8 6171.0 9 5637.0 10 5293.0 11 5032.0 12 4764.0 13 4574.0 14 4487.0 15 4475.0 16 4436.0 17 4730.0 18 5161.0 19 5752.0 20 6940.0 21 8914.0 22 11208.0 23 13689.0 24 16265.0 25 18842.0 26 21767.0 27 24355.0 28 28413.0 29 34283.0 30 42434.0 31 53189.0 32 65798.0 33 82082.0 34 103538.0 35 133254.0 36 177360.0 37 230547.0 38 187291.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.39446366287993 21.823687550594137 14.841875027661697 21.939973758864237 2 17.816324092458057 23.410912690511275 36.64003254123122 22.13273067579945 3 19.997710166859147 26.86671179960973 30.6698259726813 22.465752060849827 4 15.484321043139888 19.740637284741464 36.04823156613209 28.726810105986562 5 13.911335362409163 38.61433756934929 34.772255447271775 12.702071620969773 6 31.498574898877557 38.43648486434517 17.31817871507649 12.746761521700792 7 27.666944480791 32.83485609607627 22.23001302787054 17.268186395262177 8 26.323200400519426 35.8865972489891 20.21307462661093 17.577127723880544 9 27.133669365161538 15.252777667157819 19.8328706258498 37.78068234183084 10 15.745447356593868 29.659525990317277 33.463351291693456 21.131675361395406 11 34.962054193665836 23.045321629109612 23.427553092839222 18.56507108438533 12 22.97065315712522 25.781672232430864 30.96017965945488 20.28749495098904 13 29.47207256922123 21.9634821393015 25.7715030976749 22.792942193802375 14 22.194598838300802 21.610122484739186 27.618516643246842 28.57676203371317 15 24.24508343318767 29.36108632037064 23.837535511994965 22.556294734446723 16 23.417099506761407 28.384046263162986 26.08767074190594 22.11118348816967 17 22.454729572128322 28.217997804035793 27.164105656598984 22.163166967236897 18 22.54113166113883 27.630534711594805 28.23648713995574 21.591846487310626 19 23.670596133018254 27.295367060324434 27.860857494561635 21.173179312095677 20 23.894835501800575 26.831866512683682 27.956302587938126 21.316995397577614 21 24.175731182122803 26.532837060593824 27.54875495656428 21.742676800719092 22 23.97554856437418 26.530419224358138 27.73194160783265 21.762090603435034 23 23.78432616326368 26.56825125016356 27.56745763097561 22.07996495559715 24 23.402347275273247 26.765025697991877 27.77089921992176 22.061727806813114 25 23.701423843367273 26.70684130354702 27.676551030654377 21.915183822431327 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 903.0 1 903.0 2 821.0 3 739.0 4 739.0 5 739.0 6 1723.0 7 2707.0 8 2707.0 9 2707.0 10 3536.5 11 4366.0 12 4366.0 13 4366.0 14 6382.5 15 8399.0 16 8399.0 17 8399.0 18 13944.0 19 19489.0 20 19489.0 21 19489.0 22 28987.5 23 38486.0 24 38486.0 25 38486.0 26 52728.5 27 66971.0 28 66971.0 29 66971.0 30 82515.5 31 98060.0 32 98060.0 33 98060.0 34 111528.0 35 124996.0 36 124996.0 37 124996.0 38 136554.5 39 148113.0 40 148113.0 41 148113.0 42 158440.0 43 168767.0 44 168767.0 45 168767.0 46 172577.5 47 176388.0 48 176388.0 49 176388.0 50 174501.0 51 172614.0 52 172614.0 53 172614.0 54 157498.5 55 142383.0 56 142383.0 57 142383.0 58 122456.0 59 102529.0 60 102529.0 61 102529.0 62 84147.0 63 65765.0 64 65765.0 65 65765.0 66 51150.5 67 36536.0 68 36536.0 69 36536.0 70 26946.0 71 17356.0 72 17356.0 73 17356.0 74 12223.5 75 7091.0 76 7091.0 77 7091.0 78 4812.0 79 2533.0 80 2533.0 81 2533.0 82 1648.0 83 763.0 84 763.0 85 763.0 86 484.0 87 205.0 88 205.0 89 205.0 90 128.5 91 52.0 92 52.0 93 52.0 94 28.5 95 5.0 96 5.0 97 5.0 98 2.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38130699693361475 2 0.0 3 0.0 4 0.0 5 0.04622333979986005 6 0.0 7 0.0 8 0.0029867388793755724 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 7.111283046132316E-5 16 0.0 17 0.0 18 0.0 19 2.1333849138396947E-4 20 0.0 21 0.0 22 0.0 23 0.0 24 0.005333462284599236 25 0.0015644822701491095 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1406216.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 77.33398491662359 #Duplication Level Percentage of deduplicated Percentage of total 1 86.19663661952941 66.65929396198372 2 9.516918383515788 14.719624454470951 3 2.3113383838504573 5.362350231417132 4 0.8302487700319177 2.5682578343477442 5 0.38176951781265994 1.4761879066075452 6 0.2206511468609184 1.0238299477906776 7 0.1282735908655017 0.6943935558835695 8 0.08478168729568465 0.5245204581224299 9 0.0542578394408972 0.37763774432378355 >10 0.2592968082046326 3.2981412675879014 >50 0.009044443533463635 0.48254952946852303 >100 0.00549963345663503 0.857683452777219 >500 6.415878010147759E-4 0.36049961312271345 >1k 5.49932400869808E-4 1.146425627732357 >5k 9.1655400144968E-5 0.44860441436367543 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6329 0.45007310398971423 No Hit TATCAACGCAGAGTACTTTTTTTTT 4945 0.351652946631243 No Hit GGTATCAACGCAGAGTACTTTTTTT 4660 0.3313857899497659 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2195 0.15609266286260431 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1791 0.12736307935622976 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 7.111283046132316E-5 0.0 5 0.0 0.0 0.0 7.111283046132316E-5 0.0 6 1.422256609226463E-4 0.0 0.0 7.111283046132316E-5 0.0 7 1.422256609226463E-4 0.0 0.0 7.111283046132316E-5 0.0 8 1.422256609226463E-4 0.0 0.0 7.111283046132316E-5 0.0 9 1.422256609226463E-4 0.0 0.0 7.111283046132316E-5 0.0 10 1.422256609226463E-4 0.0 0.0 7.111283046132316E-5 0.0 11 2.1333849138396947E-4 0.0 0.0 7.111283046132316E-5 0.0 12 2.1333849138396947E-4 0.0 0.0 7.111283046132316E-5 7.111283046132316E-5 13 2.1333849138396947E-4 0.0 0.0 7.111283046132316E-5 7.111283046132316E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATTAG 100 6.2755134E-10 14.297157 1 GGTATCA 1310 0.0 13.824224 1 ACTAATC 65 5.466819E-5 13.149981 8 TCCAACG 70 1.0930285E-4 12.211565 18 ACCGTGC 80 2.8745364E-5 11.871511 8 TAGGACC 185 0.0 11.812801 4 TGTGCGG 75 2.0792136E-4 11.39665 10 GCCTCGA 120 1.2797864E-7 11.080076 16 CCTATAC 80 3.7643258E-4 10.689301 3 TAATACT 190 9.094947E-12 10.501769 4 TTGGACT 155 3.6488927E-9 10.421111 4 CCTCGAA 110 6.0639522E-6 10.360592 17 TTAGACT 120 1.5195474E-6 10.293401 4 ACTGGTC 140 9.7221346E-8 10.17558 8 TCGAACT 105 4.1135056E-5 9.950165 19 TTAGGAC 270 0.0 9.853512 3 ATTATAC 135 6.423088E-7 9.853512 3 TGGTATC 365 0.0 9.63176 2 TAGGACG 180 4.1636667E-9 9.5016 4 GCGTGCG 80 0.004525955 9.497209 9 >>END_MODULE