FastQCFastQC Report
Thu 26 May 2016
SRR1512113_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512113_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3108855
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69680.22413396572049837No Hit
TATCAACGCAGAGTACTTTTTTTTT44310.14252835851141338No Hit
GGTATCAACGCAGAGTACTTTTTTT44050.14169203774379957No Hit
GTCCTACAGTGGACATTTCTAAATT34080.10962235292414732No Hit
GTCCTAAAGTGTGTATTTCTCATTT32820.10556941381955735No Hit
CTGTAGGACGTGGAATATGGCAAGA32420.10428276648476689No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17850.012.7343121
AGCCGAT550.003175835112.0361117
TAGGACC7900.011.9708174
ACTGTCG600.006079985311.0331028
AGGACGT21750.011.0004935
GGACGTG21150.010.9995676
GGCGAGG11050.010.86906719
GCGTTAG650.007943409510.6431591
TGGCGAG22250.010.45576818
GTAGGAC21300.010.3449213
GTCCTAA12500.010.1201231
TATGGCG24150.09.98559516
TAGGACG24350.09.9812524
CTTATAC2850.09.9546793
GACGTGA13400.09.9509647
CGGTAGG1104.4360146E-59.8829341
TGTAGGA22500.09.8391742
ATGGCGA24950.09.81702917
GCGCCAC1356.863429E-79.80704413
TTAGGAC16900.09.7926953
Produced by FastQC (version 0.11.5)