FastQCFastQC Report
Thu 26 May 2016
SRR1512113_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512113_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3108855
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT89220.2869866880250124No Hit
TATCAACGCAGAGTACTTTTTTTTT57690.1855667118601543No Hit
GGTATCAACGCAGAGTACTTTTTTT57660.185470213310045No Hit
GTCCTAAAGTGTGTATTTCTCATTT38010.1222636629884636No Hit
GTCCTACAGTGGACATTTCTAAATT37870.12181333642128694No Hit
CTGTAGGACGTGGAATATGGCAAGA36580.11766389876658771No Hit
CTTTAGGACGTGAAATATGGCGAGG35280.1134822949285187No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19950.011.622561
TAGGACC9350.011.0779714
GGCGAGG10000.09.97201819
TCCAACG1752.59206E-99.76819418
TGGCGAG21150.09.56420918
ATAGGAC3800.09.5026643
TACACCG1901.0477379E-99.5026645
GTCCTAA12100.09.4631191
GTATCAA37500.09.4147511
GGTATAA2355.456968E-129.3389571
GTAGGAC24650.09.3291863
GTCTAGT2053.8926373E-109.3092471
CCCTTAT2053.8926373E-109.3092471
GTCCTAC20200.09.3057911
GGACGTG22900.09.2474356
GTAGAAC7350.09.1794443
AGGACGT23300.09.1763935
GTATAAT3850.09.1702131
TAGAACA10250.09.0854734
TGTAGGA26700.09.0755782
Produced by FastQC (version 0.11.5)