FastQCFastQC Report
Thu 26 May 2016
SRR1512112_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512112_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161807
Sequences flagged as poor quality0
Sequence length25
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT52173.224211560686497No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA51083.1568473551824088No Hit
GTACATAAGCAGTGGTATCAACGCA39112.4170771351054032No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT38752.3948284066820347No Hit
GGTATCAACGCAGAGTACTTTTTTT35082.168014980810472No Hit
TATCAACGCAGAGTACTTTTTTTTT34822.151946454726928No Hit
GTACTTTTTTTTTTTTTTTTTTTTT21981.3584084742934484No Hit
GGTATCAACGCAGAAAAAAAAAAAA18101.1186166235082535No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17431.0772092678314287No Hit
GCTTATGTACTCTGCGTTGATACCA16481.018497345603095No Hit
GAGTACTTTTTTTTTTTTTTTTTTT14340.8862410155308484No Hit
GGTATCAACGCAGAGAAAAAAAAAA12900.7972461018373742No Hit
CTTATGTACTCTGCGTTGATACCAC10990.6792042371467242No Hit
GTATCAACGCAGAGAAAAAAAAAAA10530.650775306383531No Hit
GAGTACATAAGCAGTGGTATCAACG9400.5809390199435129No Hit
GTATCAACGCAGAAAAAAAAAAAAA8140.5030684704617229No Hit
TATGTACTCTGCGTTGATACCACTG7000.43261416378772244No Hit
GCAGAGTACTTTTTTTTTTTTTTTT6750.4171636579381609No Hit
CATAAGCAGTGGTATCAACGCAGAG6210.383790565303108No Hit
TATCAACGCAGAGAAAAAAAAAAAA5990.37019412015549386No Hit
GCGTTGATACCACTGCTTATGTACT5210.321988541904862No Hit
ATCAACGCAGAGTACTTTTTTTTTT5160.31889844073494966No Hit
GTATCAACGCAGAGTACATAAGCAG5020.3102461574591952No Hit
TATCAACGCAGAGTACATAAGCAGT4930.30468397535335306No Hit
ACGCAGAGTACATAAGCAGTGGTAT4850.2997398134814934No Hit
GTACTGGTTCACTATCGGTCAGTCA4840.2991217932475109No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA4760.2941776313756512No Hit
GTGGTATCAACGCAGAGTACTTTTT4420.27316494342024755No Hit
GTGGTATCAACGCAGAGTACATGGG4370.2700748422503353No Hit
GGTATCAACGCAGAGTACATAAGCA4340.2682207815483879No Hit
CTGCTTATGTACTCTGCGTTGATAC4170.2577144375706861No Hit
TATCAACGCAGAAAAAAAAAAAAAA4080.25215225546484393No Hit
ANAAAAAAAAAAAAAAAAAAAAAAA3830.2367017496152824No Hit
GGTATCAACGCAGAGTACATGGGGG3270.20209261651226462No Hit
ACTCTGCGTTGATACCACTGCTTAT3260.20147459627828215No Hit
GNATCAACGCAGAGTACTTTTTTTT3180.19653043440642248No Hit
TNTTTTTTTTTTTTTTTTTTTTTTT3030.18726013089668556No Hit
ATCATTAACTGAATCCATAGGTTAA3010.18602409042872062No Hit
ATACAGGGTGACAGCCCCGTACACA2960.18293398925880833No Hit
GGATACCACGTGTCCCGCCCTACTC2820.17428170598305387No Hit
ACATAAGCAGTGGTATCAACGCAGA2810.17366368574907143No Hit
AAGCAGTGGTATCAACGCAGAGTAC2800.17304566551508896No Hit
GGTATCAACGCAGAGTACATGGGGA2760.1705735845791591No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT2710.16748348340924682No Hit
CCCATGTACTCTGCGTTGATACCAC2540.15697713943154498No Hit
GTGGTATCAACGCAGAAAAAAAAAA2490.15388703826163266No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT2400.14832485615579052No Hit
GTGGTATCAACGCAGAGTACATAAG2330.14399871451791332No Hit
GNTATCAACGCAGAGTACTTTTTTT2280.140908613348001No Hit
TNTCAACGCAGAGTACTTTTTTTTT2230.13781851217808871No Hit
ACGCAGAGAAAAAAAAAAAAAAAAA2170.13411039077419395No Hit
GTACATGATAAGCAGTGGTATCAAC2160.13349237054021149No Hit
CATGTACTCTGCGTTGATACCACTG2140.13225633007224658No Hit
CAACGCAGAGTACTTTTTTTTTTTT2040.12607612773242197No Hit
GGTTAATGAGGCGAACCGGGGGAAC2040.12607612773242197No Hit
GTACATGGGGGTTAAGCGACTAAGC2030.1254581074984395No Hit
GTATTTAGCCTTGGAGGATGGTCCC1970.12174998609454475No Hit
GTACATGGGGAAAAAAAAAAAAAAA1960.12113196586056227No Hit
GAGAAAAAAAAAAAAAAAAAAAAAA1950.12051394562657981No Hit
GNACATAAGCAGTGGTATCAACGCA1950.12051394562657981No Hit
GTACATGGGCAGTGGTATCAACGCA1870.11556978375472013No Hit
GTATCAACGCAGAGTACATGGGGGG1870.11556978375472013No Hit
GAATCAACGCAGAGTACTTTTTTTT1820.11247968258480782No Hit
TATTCAGACAGGATACCACGTGTCC1820.11247968258480782No Hit
GTATCAACGCAGAGTACATGGGGAA1800.1112436421168429No Hit
CCCATATTCAGACAGGATACCACGT1790.11062562188286044No Hit
TCAACGCAGAGTACTTTTTTTTTTT1760.10877156118091307No Hit
GTATCAACGCAGAGTACATGGGGGT1730.10691750047896569No Hit
TCTAAGTACCCCGAGGAAAAGAAAT1670.10320937907507093No Hit
GGATCAACGCAGAGTACTTTTTTTT1650.101973338607106No Hit
AGAAAAAAAAAAAAAAAAAAAAAAA1620.10011927790515862No Hit
GTACATGGGAAGCAGTGGTATCAAC1620.10011927790515862No Hit
GTACACAAGCAGTGGTATCAACGCA1620.10011927790515862No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAACG408.450552E-619.9234282
GATATCA250.004574971319.9169391
TGTCAAC408.474404E-619.9169391
GAACATA250.004574971319.9169391
AGGGGTT400.005448306414.17143218
GTACTGG1308.731149E-1113.0226131
TGGTTCA1251.4824764E-912.0966935
CGGTACT550.003169568512.0279624
TACATGT658.311524E-411.6314344
ACACAAG658.311524E-411.6314343
ACAAGCA658.311524E-411.6314345
ACTGGTT1302.748493E-911.6314343
GGGGTTT658.3331205E-411.6278419
TACTGGT1301.5739715E-811.4942852
GGATCAA802.4103402E-411.2032781
CTGGTTC1354.969479E-911.2006424
CACAAGC600.00606841111.0256314
GAAGCGT600.00608239711.02222416
TCTTGAG600.00608239711.02222413
GCGTCTG600.00608239711.02222419
Produced by FastQC (version 0.11.5)