##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512112_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 161807 Sequences flagged as poor quality 0 Sequence length 25 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.227456166914905 31.0 28.0 33.0 16.0 34.0 2 25.30373840439536 31.0 22.0 33.0 7.0 34.0 3 26.93546632716755 31.0 25.0 33.0 16.0 34.0 4 31.406373024652826 35.0 32.0 37.0 19.0 37.0 5 31.914639045282343 35.0 32.0 37.0 19.0 37.0 6 32.29967183125576 35.0 33.0 37.0 22.0 37.0 7 32.393431680953235 35.0 33.0 37.0 23.0 37.0 8 32.43443114327563 35.0 33.0 37.0 23.0 37.0 9 33.77410742427707 38.0 34.0 39.0 23.0 39.0 10 33.693270377672164 38.0 34.0 39.0 23.0 39.0 11 33.645274926301084 38.0 34.0 39.0 22.0 39.0 12 33.558449263628894 38.0 34.0 39.0 22.0 39.0 13 33.36672702664285 38.0 33.0 39.0 19.0 39.0 14 34.18729103190839 38.0 33.0 41.0 17.0 41.0 15 33.99095836397684 38.0 33.0 41.0 17.0 41.0 16 33.90773575926876 38.0 33.0 41.0 17.0 41.0 17 34.149857546336065 38.0 33.0 40.0 18.0 41.0 18 34.10398190436755 38.0 34.0 40.0 18.0 41.0 19 34.15886828134753 39.0 34.0 41.0 19.0 41.0 20 34.168929650756766 39.0 34.0 41.0 19.0 41.0 21 34.09261651226461 38.0 34.0 41.0 18.0 41.0 22 33.93084353581736 38.0 34.0 41.0 18.0 41.0 23 33.7124104643186 38.0 33.0 41.0 15.0 41.0 24 33.55995723299981 38.0 33.0 41.0 8.0 41.0 25 33.39671954859802 38.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 1.0610344783876684 1101 2 3.3056722080469676 1101 3 1.3878808090520245 1101 4 0.9132476215418457 1101 5 0.7549159857233079 1101 6 -0.0610496182493776 1101 7 -0.1597711423142698 1101 8 0.4054718559234729 1101 9 0.42277219254889076 1101 10 0.4332288229861234 1101 11 0.3072464795744878 1101 12 0.7299522079499852 1101 13 0.5736278857536874 1101 14 0.28167029597350535 1101 15 1.0024542879133094 1101 16 0.8893004801702844 1101 17 0.08739082913139384 1101 18 0.6005824678467349 1101 19 0.5750847387725386 1101 20 0.8231831386791697 1101 21 0.5404018332059621 1101 22 0.4587834479900508 1101 23 -0.023353073003484326 1101 24 0.2290989881216916 1101 25 0.7466651412560736 1102 1 1.6861186534718442 1102 2 4.08984729222205 1102 3 3.1313151524863656 1102 4 2.1989378572320852 1102 5 1.078990059797384 1102 6 0.7451793379795788 1102 7 0.5553803728372415 1102 8 0.2852698357214507 1102 9 0.9027385225152216 1102 10 0.49417158392888183 1102 11 0.7899064122344228 1102 12 1.1584033864011616 1102 13 0.676321488447293 1102 14 0.5863841006873116 1102 15 1.3561579916170174 1102 16 1.2229705138403233 1102 17 0.03806422980479596 1102 18 0.5157339829982561 1102 19 0.1515157152035158 1102 20 1.1240248895209248 1102 21 0.294442237246372 1102 22 0.8604669496735511 1102 23 0.8480273983769848 1102 24 0.8287622877849898 1102 25 0.9464967910877178 1103 1 1.0182682746839653 1103 2 3.0415518376765966 1103 3 1.9892696979409124 1103 4 1.2569397511714762 1103 5 1.000865059797384 1103 6 0.5039388076765476 1103 7 0.27981219101906163 1103 8 0.19696491147902861 1103 9 0.1427953406970417 1103 10 0.11633067483797532 1103 11 0.999896942537454 1103 12 0.5786211894314661 1103 13 0.36500520056850405 1103 14 0.7362420552327649 1103 15 -0.45373784171631826 1103 16 0.288548165355472 1103 17 0.5910945328351005 1103 18 1.0031866345134048 1103 19 1.2798301091429067 1103 20 1.1173961016421359 1103 21 1.2272073887615207 1103 22 0.6987718739159732 1103 23 1.1176770195891095 1103 24 0.9350596362698411 1103 25 1.0999058819968113 1104 1 1.5718235283071529 1104 2 4.265736620285292 1104 3 2.655030567506131 1104 4 1.8791091352294451 1104 5 1.5536277409568058 1104 6 1.3271951482562585 1104 7 1.3155911765263113 1104 8 1.1436361071312007 1104 9 1.4129493262042914 1104 10 1.1140661820843505 1104 11 0.7513688628273059 1104 12 0.8853467329097242 1104 13 1.1638050194090823 1104 14 1.2365817291458114 1104 15 0.7583319046604942 1104 16 1.3310074044859093 1104 17 1.076601779211913 1104 18 0.7034810185713738 1104 19 0.9657449642153679 1104 20 1.151363492946487 1104 21 0.753928403254271 1104 22 0.755723866669598 1104 23 1.6359061862557738 1104 24 1.4743485855452008 1104 25 0.9830580559098578 1105 1 0.7842811292938947 1105 2 3.6246635398042564 1105 3 1.953365442621763 1105 4 1.2861396625189947 1105 5 0.481472329300928 1105 6 0.13154297434321194 1105 7 -0.151260504016399 1105 8 0.04897067389037346 1105 9 0.31843807119349776 1105 10 0.418414008171311 1105 11 0.1056704354452549 1105 12 -0.09353084957562885 1105 13 -0.4129757391478108 1105 14 -0.7853115795190106 1105 15 -0.4186647034184432 1105 16 -0.4517554693963035 1105 17 0.044995242054959306 1105 18 0.04313565933610164 1105 19 0.09864659850460811 1105 20 0.550685108734335 1105 21 0.14276589939981932 1105 22 0.032936324270579576 1105 23 0.15363668270967423 1105 24 0.29781451215636423 1105 25 0.8264129741953923 1106 1 -0.25561886560380387 1106 2 2.444654355662209 1106 3 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-1.486219099001893 2315 20 -1.6038991472266417 2315 21 -1.6072100320529614 2315 22 -1.5048833862650213 2315 23 -1.4244256387819334 2315 24 -1.7367800130514226 2315 25 -2.1605579189081645 2316 1 -2.9006524185933458 2316 2 -3.810076313583906 2316 3 -2.6299529911347186 2316 4 -2.732753000929364 2316 5 -3.3264721250765668 2316 6 -3.04352705366799 2316 7 -3.025597472846485 2316 8 -3.126367546504163 2316 9 -2.9534494072021182 2316 10 -3.4611378125569843 2316 11 -3.400378792756662 2316 12 -3.5490705122492052 2316 13 -3.153574106154185 2316 14 -4.0599107809016886 2316 15 -4.1767551736490915 2316 16 -3.970052149770577 2316 17 -3.36268697976994 2316 18 -3.500818092377351 2316 19 -3.811687747999951 2316 20 -4.123937931971309 2316 21 -4.358664459977977 2316 22 -3.8455427269243643 2316 23 -3.833281488814251 2316 24 -3.7135091662511677 2316 25 -4.216795798675459 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8607.0 3 1.0 4 66.0 5 315.0 6 383.0 7 354.0 8 332.0 9 320.0 10 284.0 11 283.0 12 276.0 13 266.0 14 311.0 15 264.0 16 288.0 17 342.0 18 351.0 19 525.0 20 727.0 21 917.0 22 1071.0 23 1317.0 24 1507.0 25 1696.0 26 1973.0 27 2170.0 28 2583.0 29 3059.0 30 3786.0 31 4666.0 32 6100.0 33 7855.0 34 10428.0 35 14471.0 36 21843.0 37 25714.0 38 36356.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.92482401873837 18.713652684988908 15.21071399877632 14.1508092974964 2 16.873998619234325 25.67703109327984 38.766298896690074 18.682671390795765 3 18.92068946337303 34.68329553109569 32.44606228407918 13.9499527214521 4 10.384593991607286 22.770337500849777 34.63879807424895 32.20627043329399 5 8.09482902470227 43.86213204620319 32.90463329769417 15.138405631400373 6 22.50706088117325 37.03239044046302 22.33154313472223 18.1290055436415 7 19.36071986996854 43.452384631072825 20.901444313286817 16.285451185671818 8 24.226224320783167 34.25131640750538 22.534053546265852 18.988405725445602 9 29.398604510311664 17.71060584523537 19.728441909188106 33.16234773526485 10 19.522023151037963 29.347926851125106 26.04151859931894 25.088531398517986 11 26.542114988844734 30.57964117745215 20.714802202624114 22.163441631079 12 26.96731290982467 32.6920343372042 25.314108783921586 15.026543969049547 13 30.153825236238234 26.08478001569771 29.581538499570474 14.179856248493575 14 26.173936689615964 30.048947505036892 27.827151032718195 15.949964772628949 15 26.596500769435195 29.74407782110786 29.358433195102805 14.300988214354138 16 17.17231022143665 34.15736031197662 30.706953345652536 17.9633761209342 17 15.169924663333479 36.56516714357228 28.815811429666205 19.449096763428035 18 14.219409543468453 29.367085478378563 39.731284802264426 16.68222017588856 19 16.28493380962387 33.5426374794507 34.836161823418166 15.33626688750726 20 17.183540783407288 31.55383607530005 37.40837793406919 13.854245207223464 21 18.746407757389978 32.734059713115 34.340294301235424 14.179238228259594 22 16.880195296807887 31.680726800778714 33.90624517165724 17.532832730756155 23 19.37022959735484 29.860016686752573 34.71400760174284 16.05574611414975 24 15.585330581066215 32.13786880585393 33.33621744558298 18.94058316749688 25 16.814476505960805 34.5207562095583 34.49850748113493 14.166259803345962 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 10027.0 1 10027.0 2 6093.0 3 2159.0 4 2159.0 5 2159.0 6 2661.0 7 3163.0 8 3163.0 9 3163.0 10 2657.5 11 2152.0 12 2152.0 13 2152.0 14 1682.5 15 1213.0 16 1213.0 17 1213.0 18 2302.5 19 3392.0 20 3392.0 21 3392.0 22 5168.0 23 6944.0 24 6944.0 25 6944.0 26 9786.0 27 12628.0 28 12628.0 29 12628.0 30 13387.5 31 14147.0 32 14147.0 33 14147.0 34 12653.5 35 11160.0 36 11160.0 37 11160.0 38 10683.0 39 10206.0 40 10206.0 41 10206.0 42 17113.5 43 24021.0 44 24021.0 45 24021.0 46 21026.0 47 18031.0 48 18031.0 49 18031.0 50 16794.0 51 15557.0 52 15557.0 53 15557.0 54 13141.5 55 10726.0 56 10726.0 57 10726.0 58 8716.5 59 6707.0 60 6707.0 61 6707.0 62 5814.0 63 4921.0 64 4921.0 65 4921.0 66 3708.5 67 2496.0 68 2496.0 69 2496.0 70 1907.0 71 1318.0 72 1318.0 73 1318.0 74 940.0 75 562.0 76 562.0 77 562.0 78 377.5 79 193.0 80 193.0 81 193.0 82 129.5 83 66.0 84 66.0 85 66.0 86 39.0 87 12.0 88 12.0 89 12.0 90 8.5 91 5.0 92 5.0 93 5.0 94 2.5 95 0.0 96 0.0 97 0.0 98 0.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 0.0 2 5.110409314800966 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0018540607019473818 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 6.180202339824606E-4 15 0.0 16 0.0 17 0.0 18 0.0 19 6.180202339824606E-4 20 6.180202339824606E-4 21 0.0 22 0.0012360404679649213 23 0.0012360404679649213 24 6.180202339824606E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 161807.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.931826187989394 #Duplication Level Percentage of deduplicated Percentage of total 1 78.36767631272754 25.807906950873573 2 10.529970348684458 6.935423065751173 3 4.089254213114138 4.039998269543345 4 2.0474421048680704 2.697040301099458 5 1.1916826183237623 1.9622142428943123 6 0.6774762601809106 1.3386318268060096 7 0.5123296926021844 1.1810366671404822 8 0.3621964493487971 0.9542232412689191 9 0.23270652704275044 0.689710581124426 >10 1.5520024021318921 10.101540724443318 >50 0.18203655744473218 4.285352302434382 >100 0.21206320609540968 13.268894423603427 >500 0.01688998986600608 3.638903137688728 >1k 0.02251998648800811 16.71806534945954 >5k 0.003753331081334684 6.381058915868905 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5217 3.224211560686497 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 5108 3.1568473551824088 No Hit GTACATAAGCAGTGGTATCAACGCA 3911 2.4170771351054032 No Hit TTTTTTTTTTTTTTTTTTTTTTTTT 3875 2.3948284066820347 No Hit GGTATCAACGCAGAGTACTTTTTTT 3508 2.168014980810472 No Hit TATCAACGCAGAGTACTTTTTTTTT 3482 2.151946454726928 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 2198 1.3584084742934484 No Hit GGTATCAACGCAGAAAAAAAAAAAA 1810 1.1186166235082535 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1743 1.0772092678314287 No Hit GCTTATGTACTCTGCGTTGATACCA 1648 1.018497345603095 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1434 0.8862410155308484 No Hit GGTATCAACGCAGAGAAAAAAAAAA 1290 0.7972461018373742 No Hit CTTATGTACTCTGCGTTGATACCAC 1099 0.6792042371467242 No Hit GTATCAACGCAGAGAAAAAAAAAAA 1053 0.650775306383531 No Hit GAGTACATAAGCAGTGGTATCAACG 940 0.5809390199435129 No Hit GTATCAACGCAGAAAAAAAAAAAAA 814 0.5030684704617229 No Hit TATGTACTCTGCGTTGATACCACTG 700 0.43261416378772244 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 675 0.4171636579381609 No Hit CATAAGCAGTGGTATCAACGCAGAG 621 0.383790565303108 No Hit TATCAACGCAGAGAAAAAAAAAAAA 599 0.37019412015549386 No Hit GCGTTGATACCACTGCTTATGTACT 521 0.321988541904862 No Hit ATCAACGCAGAGTACTTTTTTTTTT 516 0.31889844073494966 No Hit GTATCAACGCAGAGTACATAAGCAG 502 0.3102461574591952 No Hit TATCAACGCAGAGTACATAAGCAGT 493 0.30468397535335306 No Hit ACGCAGAGTACATAAGCAGTGGTAT 485 0.2997398134814934 No Hit GTACTGGTTCACTATCGGTCAGTCA 484 0.2991217932475109 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 476 0.2941776313756512 No Hit GTGGTATCAACGCAGAGTACTTTTT 442 0.27316494342024755 No Hit GTGGTATCAACGCAGAGTACATGGG 437 0.2700748422503353 No Hit GGTATCAACGCAGAGTACATAAGCA 434 0.2682207815483879 No Hit CTGCTTATGTACTCTGCGTTGATAC 417 0.2577144375706861 No Hit TATCAACGCAGAAAAAAAAAAAAAA 408 0.25215225546484393 No Hit ANAAAAAAAAAAAAAAAAAAAAAAA 383 0.2367017496152824 No Hit GGTATCAACGCAGAGTACATGGGGG 327 0.20209261651226462 No Hit ACTCTGCGTTGATACCACTGCTTAT 326 0.20147459627828215 No Hit GNATCAACGCAGAGTACTTTTTTTT 318 0.19653043440642248 No Hit TNTTTTTTTTTTTTTTTTTTTTTTT 303 0.18726013089668556 No Hit ATCATTAACTGAATCCATAGGTTAA 301 0.18602409042872062 No Hit ATACAGGGTGACAGCCCCGTACACA 296 0.18293398925880833 No Hit GGATACCACGTGTCCCGCCCTACTC 282 0.17428170598305387 No Hit ACATAAGCAGTGGTATCAACGCAGA 281 0.17366368574907143 No Hit AAGCAGTGGTATCAACGCAGAGTAC 280 0.17304566551508896 No Hit GGTATCAACGCAGAGTACATGGGGA 276 0.1705735845791591 No Hit CTTTTTTTTTTTTTTTTTTTTTTTT 271 0.16748348340924682 No Hit CCCATGTACTCTGCGTTGATACCAC 254 0.15697713943154498 No Hit GTGGTATCAACGCAGAAAAAAAAAA 249 0.15388703826163266 No Hit ACTTTTTTTTTTTTTTTTTTTTTTT 240 0.14832485615579052 No Hit GTGGTATCAACGCAGAGTACATAAG 233 0.14399871451791332 No Hit GNTATCAACGCAGAGTACTTTTTTT 228 0.140908613348001 No Hit TNTCAACGCAGAGTACTTTTTTTTT 223 0.13781851217808871 No Hit ACGCAGAGAAAAAAAAAAAAAAAAA 217 0.13411039077419395 No Hit GTACATGATAAGCAGTGGTATCAAC 216 0.13349237054021149 No Hit CATGTACTCTGCGTTGATACCACTG 214 0.13225633007224658 No Hit CAACGCAGAGTACTTTTTTTTTTTT 204 0.12607612773242197 No Hit GGTTAATGAGGCGAACCGGGGGAAC 204 0.12607612773242197 No Hit GTACATGGGGGTTAAGCGACTAAGC 203 0.1254581074984395 No Hit GTATTTAGCCTTGGAGGATGGTCCC 197 0.12174998609454475 No Hit GTACATGGGGAAAAAAAAAAAAAAA 196 0.12113196586056227 No Hit GAGAAAAAAAAAAAAAAAAAAAAAA 195 0.12051394562657981 No Hit GNACATAAGCAGTGGTATCAACGCA 195 0.12051394562657981 No Hit GTACATGGGCAGTGGTATCAACGCA 187 0.11556978375472013 No Hit GTATCAACGCAGAGTACATGGGGGG 187 0.11556978375472013 No Hit GAATCAACGCAGAGTACTTTTTTTT 182 0.11247968258480782 No Hit TATTCAGACAGGATACCACGTGTCC 182 0.11247968258480782 No Hit GTATCAACGCAGAGTACATGGGGAA 180 0.1112436421168429 No Hit CCCATATTCAGACAGGATACCACGT 179 0.11062562188286044 No Hit TCAACGCAGAGTACTTTTTTTTTTT 176 0.10877156118091307 No Hit GTATCAACGCAGAGTACATGGGGGT 173 0.10691750047896569 No Hit TCTAAGTACCCCGAGGAAAAGAAAT 167 0.10320937907507093 No Hit GGATCAACGCAGAGTACTTTTTTTT 165 0.101973338607106 No Hit AGAAAAAAAAAAAAAAAAAAAAAAA 162 0.10011927790515862 No Hit GTACATGGGAAGCAGTGGTATCAAC 162 0.10011927790515862 No Hit GTACACAAGCAGTGGTATCAACGCA 162 0.10011927790515862 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 6.180202339824606E-4 0.0 0.0 0.0 0.0 9 6.180202339824606E-4 0.0 0.0 0.0 0.0 10 6.180202339824606E-4 0.0 0.0 0.0 0.0 11 6.180202339824606E-4 0.0 0.0 0.0 0.0 12 6.180202339824606E-4 0.0 0.0 0.0 0.0 13 6.180202339824606E-4 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCAACG 40 8.450552E-6 19.923428 2 GATATCA 25 0.0045749713 19.916939 1 TGTCAAC 40 8.474404E-6 19.916939 1 GAACATA 25 0.0045749713 19.916939 1 AGGGGTT 40 0.0054483064 14.171432 18 GTACTGG 130 8.731149E-11 13.022613 1 TGGTTCA 125 1.4824764E-9 12.096693 5 CGGTACT 55 0.0031695685 12.027962 4 TACATGT 65 8.311524E-4 11.631434 4 ACACAAG 65 8.311524E-4 11.631434 3 ACAAGCA 65 8.311524E-4 11.631434 5 ACTGGTT 130 2.748493E-9 11.631434 3 GGGGTTT 65 8.3331205E-4 11.62784 19 TACTGGT 130 1.5739715E-8 11.494285 2 GGATCAA 80 2.4103402E-4 11.203278 1 CTGGTTC 135 4.969479E-9 11.200642 4 CACAAGC 60 0.006068411 11.025631 4 GAAGCGT 60 0.006082397 11.022224 16 TCTTGAG 60 0.006082397 11.022224 13 GCGTCTG 60 0.006082397 11.022224 19 >>END_MODULE