FastQCFastQC Report
Thu 26 May 2016
SRR1512112_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512112_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161807
Sequences flagged as poor quality0
Sequence length25
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT70364.348390366300593No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT57713.56659477031278No Hit
TATCAACGCAGAGTACTTTTTTTTT52643.2532585116836725No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA52513.2452242486419007No Hit
GGTATCAACGCAGAGTACTTTTTTT48472.9955440741129866No Hit
GTACATAAGCAGTGGTATCAACGCA43322.677263653612019No Hit
GTACTTTTTTTTTTTTTTTTTTTTT29671.8336660342259608No Hit
ACGCAGAGTACTTTTTTTTTTTTTT25791.5938741834407657No Hit
GCTTATGTACTCTGCGTTGATACCA19121.1816546873744647No Hit
GAGTACTTTTTTTTTTTTTTTTTTT17681.0926597736809902No Hit
GGTATCAACGCAGAAAAAAAAAAAA15790.9758539494583054No Hit
CTTATGTACTCTGCGTTGATACCAC12140.7502765640547072No Hit
GGTATCAACGCAGAGAAAAAAAAAA11170.6903286013584085No Hit
GAGTACATAAGCAGTGGTATCAACG10500.6489212456815836No Hit
GCAGAGTACTTTTTTTTTTTTTTTT10260.6340887600660046No Hit
GTATCAACGCAGAGAAAAAAAAAAA9260.5722867366677585No Hit
TATGTACTCTGCGTTGATACCACTG8970.5543641498822671No Hit
ATCAACGCAGAGTACTTTTTTTTTT7700.4758755801664946No Hit
CATAAGCAGTGGTATCAACGCAGAG7670.47402151946454724No Hit
GTATCAACGCAGAAAAAAAAAAAAA7370.45548091244507344No Hit
GTGGTATCAACGCAGAGTACTTTTT6820.4214897995760381No Hit
GCGTTGATACCACTGCTTATGTACT6640.4103654353643538No Hit
TATCAACGCAGAGAAAAAAAAAAAA6030.37266620109142373No Hit
TATCAACGCAGAGTACATAAGCAGT5820.3596877761777921No Hit
ACGCAGAGTACATAAGCAGTGGTAT5240.32384260260680936No Hit
GTACTGGTTCACTATCGGTCAGTCA5180.3201344812029146No Hit
GTATCAACGCAGAGTACATAAGCAG5150.3182804205009672No Hit
GTGGTATCAACGCAGAGTACATGGG5000.3090101169912303No Hit
GGTATCAACGCAGAGTACATAAGCA4710.2910875302057389No Hit
CTGCTTATGTACTCTGCGTTGATAC4640.2867613885678617No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA4420.27316494342024755No Hit
TATCAACGCAGAAAAAAAAAAAAAA4260.2632766196765282No Hit
AAGCAGTGGTATCAACGCAGAGTAC3960.24473601265705439No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT3760.23237560797740517No Hit
ACTCTGCGTTGATACCACTGCTTAT3750.23175758774342273No Hit
GGTATCAACGCAGAGTACATGGGGG3620.22372332470165074No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT3420.21136292002200152No Hit
ACATAAGCAGTGGTATCAACGCAGA3190.1971484546404049No Hit
CCCATGTACTCTGCGTTGATACCAC3130.19344033323651016No Hit
GGATACCACGTGTCCCGCCCTACTC3130.19344033323651016No Hit
GTGGTATCAACGCAGAGTACATAAG3040.187878151130668No Hit
CATGTACTCTGCGTTGATACCACTG2980.18417002972677324No Hit
ATACAGGGTGACAGCCCCGTACACA2900.17922586785491357No Hit
ATCATTAACTGAATCCATAGGTTAA2900.17922586785491357No Hit
AACGCAGAGTACTTTTTTTTTTTTT2890.1786078476209311No Hit
CAACGCAGAGTACTTTTTTTTTTTT2850.17613576668500125No Hit
GGTATCAACGCAGAGTACATGGGGA2800.17304566551508896No Hit
TCAACGCAGAGTACTTTTTTTTTTT2630.16253932153738715No Hit
GGTTAATGAGGCGAACCGGGGGAAC2420.14956089662375546No Hit
GTATCAACGCAGAGTACATGGGGGT2350.14523475498587823No Hit
GTACATGGGGGTTAAGCGACTAAGC2300.14214465381596594No Hit
GTGGTATCAACGCAGAAAAAAAAAA2260.1396725728800361No Hit
GTACATGATAAGCAGTGGTATCAAC2240.13843653241207116No Hit
GTACATGGGCAGTGGTATCAACGCA2200.13596445147614133No Hit
GTATTTAGCCTTGGAGGATGGTCCC2140.13225633007224658No Hit
TCTAAGTACCCCGAGGAAAAGAAAT2100.12978424913631673No Hit
GTACACAAGCAGTGGTATCAACGCA2030.1254581074984395No Hit
TATTCAGACAGGATACCACGTGTCC2020.12484008726445703No Hit
CTATCGGTCAGTCAGGAGTATTTAG2000.12360404679649213No Hit
GAGAAAAAAAAAAAAAAAAAAAAAA1990.12298602656250965No Hit
GTTCACTATCGGTCAGTCAGGAGTA1870.11556978375472013No Hit
CAGTGGTATCAACGCAGAGTACATA1850.1143337432867552No Hit
ACGCAGAGAAAAAAAAAAAAAAAAA1850.1143337432867552No Hit
GCAGTGGTATCAACGCAGAGTACAT1850.1143337432867552No Hit
CCACTGCTTATGTACTCTGCGTTGA1820.11247968258480782No Hit
GTATCAACGCAGAGTACATGGGGGG1800.1112436421168429No Hit
GTCAGGAGTATTTAGCCTTGGAGGA1790.11062562188286044No Hit
GTATCAACGCAGAGTACATGGGGAA1760.10877156118091307No Hit
CCCATATTCAGACAGGATACCACGT1730.10691750047896569No Hit
GTACTCTGCGTTGATACCACTGCTT1700.1050634397770183No Hit
TCACGGTACTGGTTCACTATCGGTC1660.10259135884108846No Hit
CGGTACTGGTTCACTATCGGTCAGT1650.101973338607106No Hit
GTACATGGGAAGCAGTGGTATCAAC1650.101973338607106No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGGGT601.4416873E-615.8390033
TACAGGG653.3148772E-614.6206182
GGGTCTT400.005261183314.24629110
TGAGGGG400.005261183314.24629116
AGCCCCG759.547548E-713.92970713
GTGTCCC655.4070384E-513.15042410
CAGCCCC801.9738018E-613.059112
CCCCGTA801.9738018E-613.059115
CCGTACA801.9738018E-613.059117
GCCCCGT801.9738018E-613.059114
ATACAGG751.4263891E-512.6947551
CAGGGTG751.4539293E-512.6712024
ACAGCCC751.4632145E-512.6633711
GGGTGAC751.4632145E-512.663376
CCCGTAC853.893354E-612.29091916
TGTCCCG701.08192675E-412.21110711
AAACACA550.003054016312.08776319
AGGGTGA802.8189977E-511.87925155
GGTGACA802.8369172E-511.8719097
GACAGCC802.8369172E-511.87190910
Produced by FastQC (version 0.11.5)