FastQCFastQC Report
Thu 26 May 2016
SRR1512111_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512111_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2302824
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT55400.24057418196093144No Hit
GGTATCAACGCAGAGTACTTTTTTT35980.15624294344682876No Hit
TATCAACGCAGAGTACTTTTTTTTT35910.1559389688486832No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACTGCG509.072858E-515.1288167
GTGCGCC1001.0766598E-813.23771411
GGTATCA13450.012.7283831
ATGCGTC550.00317901212.03428657
GTCTTAG2750.011.5150741
TACACCG752.1670679E-411.3466135
CTAGTAC1604.9112714E-1111.2284183
GTATCAA27350.010.4565871
TAGGACC4100.010.3779994
TTAGGAC7350.010.1630663
CCAACGA1252.729923E-69.83373119
GTCCTAC10450.09.564331
GACGTGA5550.09.5406957
GACCTAC1157.115604E-59.4655161
AGCGTGC1107.198754E-59.4555118
CGTGCGC1107.198754E-59.45551110
ATGTCGA900.00116279429.4555117
GGACGTG9250.09.3021786
GAATAAG2900.09.21331
TAGGACG10000.09.1718454
Produced by FastQC (version 0.11.5)