FastQCFastQC Report
Thu 26 May 2016
SRR1512111_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512111_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2302824
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT75520.3279451664564899No Hit
TATCAACGCAGAGTACTTTTTTTTT49540.2151271656018871No Hit
GGTATCAACGCAGAGTACTTTTTTT48370.21004644731859665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4800.012.2727834
GGTATCA16450.012.1213321
ACCGTGC909.549542E-510.552198
GTATAAG2950.010.3489891
AACCGTG750.002655414410.1301027
GGACCGT750.002655414410.1301026
AGTCTCG750.00265637110.129662510
TGTACCG1356.431601E-79.8534175
GGCGTGC1554.0854502E-89.8033268
GGATAGG1502.4905785E-79.5404751
TGGAGCG1106.82665E-59.501515
GTAGGAC11400.09.501513
GCACTCG800.00453036239.49655913
GTATCAA30550.09.493631
GTCCTAT3400.09.2598721
TGTAGGA12300.09.1925172
TAGACTA1451.7031125E-69.1738725
GTCCTAC13000.09.17353251
GACCGTG1151.11477064E-49.0840597
CGAAATC1151.1152981E-49.08366413
Produced by FastQC (version 0.11.5)