FastQCFastQC Report
Thu 26 May 2016
SRR1512110_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512110_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3165884
Sequences flagged as poor quality0
Sequence length25
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT69010.21798019131465335No Hit
TATCAACGCAGAGTACTTTTTTTTT44870.14172976647280824No Hit
GGTATCAACGCAGAGTACTTTTTTT44790.14147707243853536No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17200.013.8515651
TATACCG1053.6650836E-610.8074975
TAGACGG909.9740224E-510.5072895
GTCCTAC11300.010.1482191
GTATCAA35750.010.12081051
TAGGACC5650.09.8749914
TAGGACG12850.09.8613154
AGGACGT12750.09.6419835
TTAGACG800.00469164749.456564
TAGAACG800.00469164749.456564
CTAATAC2800.09.4565593
TTAGGAC9000.09.3514873
CTAGGAC3750.09.3304723
GGACGTG12550.09.2681836
TAGAACA8000.09.2201464
GTCCTAA8150.09.2186271
GTAGGAC12750.09.1969683
TCCTACA13300.09.1424742
GACGTGA6550.09.0956237
CTTATAC2401.0913936E-119.0625373
Produced by FastQC (version 0.11.5)