##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512110_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3165884 Sequences flagged as poor quality 0 Sequence length 25 %GC 47 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.136389393925995 31.0 28.0 33.0 16.0 34.0 2 25.686407019334883 31.0 23.0 33.0 7.0 34.0 3 27.298613910048505 31.0 26.0 34.0 16.0 34.0 4 31.485063571501673 35.0 32.0 37.0 19.0 37.0 5 31.84381266022381 35.0 32.0 37.0 19.0 37.0 6 32.07835094400174 35.0 33.0 37.0 19.0 37.0 7 32.09745271778751 35.0 33.0 37.0 19.0 37.0 8 32.12697496181161 35.0 33.0 37.0 19.0 37.0 9 33.59302646590968 38.0 34.0 39.0 21.0 39.0 10 33.43112982029664 38.0 33.0 39.0 19.0 39.0 11 33.437294291262724 38.0 33.0 39.0 19.0 39.0 12 33.30817048255716 38.0 33.0 39.0 18.0 39.0 13 33.29076712854925 38.0 33.0 39.0 17.0 39.0 14 34.26884781628133 39.0 33.0 41.0 17.0 41.0 15 34.17987361507876 39.0 33.0 41.0 17.0 41.0 16 34.12922046417367 39.0 33.0 41.0 17.0 41.0 17 34.04297441093862 39.0 33.0 41.0 17.0 41.0 18 33.984824459771744 39.0 33.0 41.0 16.0 41.0 19 33.99128868903598 39.0 34.0 41.0 13.0 41.0 20 33.900362110551114 39.0 33.0 41.0 10.0 41.0 21 33.822552247650265 39.0 33.0 41.0 10.0 41.0 22 33.685798026712284 39.0 33.0 41.0 8.0 41.0 23 33.54937736189955 39.0 33.0 41.0 2.0 41.0 24 33.40011225932473 39.0 33.0 41.0 2.0 41.0 25 33.24577779855484 39.0 33.0 41.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.9152188550785212 1101 2 3.1737613058619054 1101 3 1.805500327063907 1101 4 1.176747732075313 1101 5 0.9282709423322046 1101 6 0.43640621285391745 1101 7 0.29439715709196435 1101 8 0.311522349203905 1101 9 0.3271913725995006 1101 10 0.20272103382186657 1101 11 0.40029114001929145 1101 12 0.004064739745139434 1101 13 0.16854468268434886 1101 14 0.2509869322915037 1101 15 0.34526870747799165 1101 16 0.188926237225445 1101 17 0.1340367518620269 1101 18 -0.06006149366814384 1101 19 0.08218344936121014 1101 20 0.044294379592628275 1101 21 0.0900558845482351 1101 22 0.21243799117728912 1101 23 0.10272537711238527 1101 24 0.03420474871054324 1101 25 0.023227696249371377 1102 1 1.4482910683968164 1102 2 3.8203573470771914 1102 3 2.4975700819091244 1102 4 1.6593056199649823 1102 5 1.326927871565779 1102 6 1.1065191319568015 1102 7 0.9878012642754541 1102 8 0.833402512244696 1102 9 0.9605820867933517 1102 10 0.8805528534876714 1102 11 1.0433964091652044 1102 12 1.0177786417425878 1102 13 1.0363575444063713 1102 14 1.0552449260389665 1102 15 1.0263683918832598 1102 16 1.1386808845804879 1102 17 0.8282585981406783 1102 18 0.8155911517873875 1102 19 0.9364227695958789 1102 20 1.0712819338818491 1102 21 1.080865362778134 1102 22 1.0530796551233124 1102 23 0.9844317651850858 1102 24 0.9429932952929221 1102 25 0.8534186854756172 1103 1 1.0969398674706774 1103 2 2.9221331339128014 1103 3 1.9595362486139578 1103 4 1.281647584954932 1103 5 0.9567788053367394 1103 6 0.9277931123145322 1103 7 0.7828749545789933 1103 8 0.5932236454915198 1103 9 0.8360337062626044 1103 10 0.7166197567774972 1103 11 0.8264648136127022 1103 12 0.7228345535050451 1103 13 0.8034196961505131 1103 14 0.7741412253036586 1103 15 0.6488844848023732 1103 16 0.46481374975617484 1103 17 0.6789729202026109 1103 18 0.7004957710276543 1103 19 0.7624371641713523 1103 20 0.7995723951697897 1103 21 0.9425386129117115 1103 22 0.7407283397021018 1103 23 0.7560172675640402 1103 24 0.7370977529679053 1103 25 0.7117310280448876 1104 1 1.4090051545686002 1104 2 3.837996637299451 1104 3 2.3654956959491855 1104 4 1.7810289493951572 1104 5 1.2876114642706398 1104 6 1.0440780532041742 1104 7 0.8434149934966158 1104 8 0.8231138197730772 1104 9 0.9891271195453015 1104 10 1.0199766568059943 1104 11 0.9519752247438689 1104 12 0.9083816569365979 1104 13 0.8975590538920031 1104 14 0.9336260613245102 1104 15 0.8142522947952031 1104 16 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-2.5559276620777496 2315 21 -2.590536152373378 2315 22 -2.706926658008001 2315 23 -2.5111806085522907 2315 24 -2.465150644101019 2315 25 -2.5090813145243835 2316 1 -1.9434120471531564 2316 2 -2.606408191675275 2316 3 -1.756088233157005 2316 4 -2.1202298628775296 2316 5 -2.164855051815966 2316 6 -2.327729631073119 2316 7 -2.393207841274762 2316 8 -2.4953575210330854 2316 9 -2.7672639706381865 2316 10 -2.85186188588656 2316 11 -3.253772140323175 2316 12 -3.0874194034615527 2316 13 -3.1413025399101073 2316 14 -3.333563658998621 2316 15 -3.5085777232917934 2316 16 -3.4974494515633943 2316 17 -3.3969404254192206 2316 18 -3.3157401954153336 2316 19 -3.5643711182120335 2316 20 -3.544187649516484 2316 21 -3.5414379158767417 2316 22 -3.612340548675718 2316 23 -3.6667618288424286 2316 24 -3.4631764008557795 2316 25 -3.8334886567080737 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 182384.0 3 3.0 4 1589.0 5 7487.0 6 8608.0 7 7804.0 8 7351.0 9 6882.0 10 6609.0 11 6437.0 12 6492.0 13 6333.0 14 6529.0 15 6827.0 16 7024.0 17 7581.0 18 8688.0 19 10811.0 20 14250.0 21 17795.0 22 21617.0 23 25865.0 24 30185.0 25 33976.0 26 37734.0 27 42175.0 28 48526.0 29 57647.0 30 71101.0 31 88384.0 32 111036.0 33 141643.0 34 183459.0 35 243470.0 36 337174.0 37 463292.0 38 901116.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.74465394183741 18.384059554929998 12.723681600462935 25.147604902769654 2 16.73894272124051 21.47693445624555 38.028420619891136 23.755702202622807 3 18.47474512647968 24.72487937018539 29.775601380214816 27.024774123120114 4 12.850344485142223 16.929900147952356 37.59967200314351 32.62008336376191 5 13.833103171183783 37.43504183981472 35.06508766587784 13.666767323123652 6 31.948359447155994 36.59192819446322 18.109254792658227 13.350457565722559 7 28.08299356514642 31.75580659304005 22.216038237661266 17.94516160415227 8 25.977249884234183 35.39385964191879 20.52396905246387 18.104921421383164 9 26.739798425968857 15.35046767348393 19.84298856180454 38.06674533874267 10 15.61320629561917 28.42112976975783 33.67492302307981 22.29074091154319 11 34.34898435950275 22.585855956819643 24.074129058424123 18.991030625253483 12 23.3018329161776 24.628129141813154 30.972644607319786 21.097393334689457 13 28.8651131879753 21.565856487477113 25.663637707509185 23.9053926170384 14 22.028703606018432 21.161012725080024 27.144415728484457 29.665867940417083 15 23.72436892823616 29.049895700537355 23.513021955321168 23.71271341590532 16 23.357267669946214 27.56970880802961 26.163561267563818 22.909462254460365 17 22.65058437093222 27.630048236147704 26.962146105841562 22.75722128707852 18 22.496117987898483 27.166156435295797 28.100871668071225 22.236853908734496 19 23.913868857533632 26.650115134228507 27.08715139914147 22.348864609096395 20 23.943975170268505 26.62417613796092 27.34144860737071 22.09040008439986 21 23.944172254105574 26.197099638299733 26.83502633232266 23.023701775272034 22 23.923972584999078 26.499640855654295 26.938573997949373 22.637812561397258 23 23.568111479556546 26.448534897692692 26.97117428662219 23.012179336128575 24 23.49691443523757 26.372837048635272 27.19002132430538 22.940227191821776 25 23.555071365555836 26.39683778585627 27.09986439802103 22.94822645056687 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 655.0 1 655.0 2 824.5 3 994.0 4 994.0 5 994.0 6 2365.5 7 3737.0 8 3737.0 9 3737.0 10 5228.5 11 6720.0 12 6720.0 13 6720.0 14 10589.5 15 14459.0 16 14459.0 17 14459.0 18 24718.0 19 34977.0 20 34977.0 21 34977.0 22 53507.5 23 72038.0 24 72038.0 25 72038.0 26 100494.0 27 128950.0 28 128950.0 29 128950.0 30 164785.0 31 200620.0 32 200620.0 33 200620.0 34 232481.0 35 264342.0 36 264342.0 37 264342.0 38 294843.0 39 325344.0 40 325344.0 41 325344.0 42 351930.0 43 378516.0 44 378516.0 45 378516.0 46 394770.5 47 411025.0 48 411025.0 49 411025.0 50 408671.0 51 406317.0 52 406317.0 53 406317.0 54 374339.0 55 342361.0 56 342361.0 57 342361.0 58 296478.5 59 250596.0 60 250596.0 61 250596.0 62 207193.0 63 163790.0 64 163790.0 65 163790.0 66 127856.5 67 91923.0 68 91923.0 69 91923.0 70 67327.5 71 42732.0 72 42732.0 73 42732.0 74 29920.5 75 17109.0 76 17109.0 77 17109.0 78 11599.5 79 6090.0 80 6090.0 81 6090.0 82 3973.5 83 1857.0 84 1857.0 85 1857.0 86 1189.0 87 521.0 88 521.0 89 521.0 90 341.5 91 162.0 92 162.0 93 162.0 94 100.5 95 39.0 96 39.0 97 39.0 98 24.5 99 10.0 100 10.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.34156778959684 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 3.1586754284111484E-4 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 3.1586754284111484E-4 15 0.0 16 0.0 17 3.158675428411149E-5 18 0.0 19 4.422145599775608E-4 20 6.317350856822298E-5 21 9.476026285233446E-5 22 8.212556113868985E-4 23 9.476026285233446E-4 24 4.1062780569344924E-4 25 3.158675428411149E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 3165884.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.1767496501337 #Duplication Level Percentage of deduplicated Percentage of total 1 82.12146278635531 57.63017334860828 2 11.444900151355718 16.06331785384935 3 3.077006064301419 6.478028527392716 4 1.278137248009315 3.5878207068824217 5 0.6366160702662631 2.2337823293163734 6 0.3693453791334817 1.5551674923530436 7 0.24934977903655525 1.2248989913135147 8 0.16559573299494548 0.929677623801334 9 0.12320907447286958 0.7781771136516522 >10 0.5043986826055794 5.89476041720626 >50 0.019653630888625453 0.9442672729438988 >100 0.008783455638589937 1.1563500522761863 >500 8.616751158593597E-4 0.41322986938138445 >1k 6.349185038797707E-4 0.8907165988147087 >5k 4.5351321705697915E-5 0.21963180220889358 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 6901 0.21798019131465335 No Hit TATCAACGCAGAGTACTTTTTTTTT 4487 0.14172976647280824 No Hit GGTATCAACGCAGAGTACTTTTTTT 4479 0.14147707243853536 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 3.158675428411148E-5 0.0 0.0 0.0 4 0.0 3.158675428411148E-5 0.0 0.0 0.0 5 3.158675428411148E-5 1.2634701713644592E-4 0.0 0.0 0.0 6 3.158675428411148E-5 1.2634701713644592E-4 0.0 0.0 0.0 7 3.158675428411148E-5 1.2634701713644592E-4 0.0 0.0 0.0 8 3.158675428411148E-5 1.2634701713644592E-4 0.0 0.0 0.0 9 3.158675428411148E-5 1.5793377142055742E-4 0.0 0.0 0.0 10 3.158675428411148E-5 1.5793377142055742E-4 0.0 0.0 0.0 11 3.158675428411148E-5 1.5793377142055742E-4 0.0 0.0 0.0 12 3.158675428411148E-5 1.5793377142055742E-4 0.0 0.0 0.0 13 3.158675428411148E-5 2.5269403427289185E-4 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1720 0.0 13.851565 1 TATACCG 105 3.6650836E-6 10.807497 5 TAGACGG 90 9.9740224E-5 10.507289 5 GTCCTAC 1130 0.0 10.148219 1 GTATCAA 3575 0.0 10.1208105 1 TAGGACC 565 0.0 9.874991 4 TAGGACG 1285 0.0 9.861315 4 AGGACGT 1275 0.0 9.641983 5 TTAGACG 80 0.0046916474 9.45656 4 TAGAACG 80 0.0046916474 9.45656 4 CTAATAC 280 0.0 9.456559 3 TTAGGAC 900 0.0 9.351487 3 CTAGGAC 375 0.0 9.330472 3 GGACGTG 1255 0.0 9.268183 6 TAGAACA 800 0.0 9.220146 4 GTCCTAA 815 0.0 9.218627 1 GTAGGAC 1275 0.0 9.196968 3 TCCTACA 1330 0.0 9.142474 2 GACGTGA 655 0.0 9.095623 7 CTTATAC 240 1.0913936E-11 9.062537 3 >>END_MODULE