FastQCFastQC Report
Thu 26 May 2016
SRR1512110_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512110_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3165884
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT87280.27568919139172504No Hit
TATCAACGCAGAGTACTTTTTTTTT57870.18279254704215317No Hit
GGTATCAACGCAGAGTACTTTTTTT56790.17938117757946911No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19500.013.7475321
GTTCTAA3000.010.8121161
TAGGACC4650.010.6263514
GTCCTAA8000.010.2556111
GATATAC4950.010.2146551
CGTGCCG750.00265754210.12934110
GTCCTAC11650.09.7448251
GTATCAA40200.09.7299551
TTAGGAC9600.09.70037653
GGCGAGG7550.09.56080619
GTATTAA3600.09.5401031
TGTACCG1401.0558306E-69.5024115
GTCCTAG4000.09.30161
TGGCGAG13750.09.25570418
AGGACGT12350.09.233115
TATGGCG14350.09.19846416
GGACGTG12350.09.150246
TAGGACG13100.09.0671854
TATGCCG950.00182034029.0022825
ATAACCG950.00182034029.0022825
Produced by FastQC (version 0.11.5)