FastQCFastQC Report
Thu 26 May 2016
SRR1512109_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512109_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2850618
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT49710.17438323900291094No Hit
GGTATCAACGCAGAGTACTTTTTTT30070.10548589814559509No Hit
TATCAACGCAGAGTACTTTTTTTTT29420.10320569083616254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA12400.012.6839071
GTATCAA27900.010.74276351
TATGTCG1159.3700146E-710.68920316
TCCAACG1605.9117156E-1010.63818
TGGACGG1106.3955886E-610.3154555
AAGGCGT2053.6379788E-1210.1477246
CCAACGA1401.037879E-710.13142919
CGTATAG750.002752696210.086410
GTAGGAC14950.09.9934573
GTCCTAC15250.09.8594191
TAGGACC6200.09.7608614
TGTAGGA16450.09.4408562
GTATAAA5000.09.298341
GTACTAG1501.5626283E-69.2323951
TAGGACG13450.09.1394684
GTCCTAT3150.09.1067831
GGCGAGG6150.09.071609519
AGGACGT12300.09.071455
GGACGTG12250.08.8769056
GTGCGCC1607.212184E-78.86511
Produced by FastQC (version 0.11.5)