##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512109_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2850618 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 27.987027725216077 31.0 28.0 33.0 16.0 34.0 2 25.538179440388014 31.0 23.0 33.0 2.0 34.0 3 27.157967500380618 31.0 26.0 34.0 16.0 34.0 4 31.317924043137314 35.0 32.0 37.0 19.0 37.0 5 31.683115380594664 35.0 32.0 37.0 19.0 37.0 6 31.909878489506486 35.0 32.0 37.0 17.0 37.0 7 31.9252004302225 35.0 32.0 37.0 17.0 37.0 8 31.950646140591267 35.0 33.0 37.0 17.0 37.0 9 33.39949898583395 38.0 33.0 39.0 18.0 39.0 10 33.23001714014294 38.0 33.0 39.0 17.0 39.0 11 33.23566363504335 38.0 33.0 39.0 17.0 39.0 12 33.10559148928408 38.0 33.0 39.0 17.0 39.0 13 33.087153732980006 38.0 33.0 39.0 17.0 39.0 14 34.05107980094141 39.0 33.0 41.0 17.0 41.0 15 33.96829985638202 39.0 33.0 41.0 16.0 41.0 16 33.920142579609056 39.0 33.0 41.0 16.0 41.0 17 33.83735526822605 39.0 33.0 41.0 11.0 41.0 18 33.776150996029635 39.0 33.0 41.0 10.0 41.0 19 33.78114815804854 39.0 33.0 41.0 10.0 41.0 20 33.694111241843 39.0 33.0 41.0 10.0 41.0 21 33.6119848397786 39.0 33.0 41.0 8.0 41.0 22 33.478458355346106 39.0 33.0 41.0 2.0 41.0 23 33.338783730405126 39.0 33.0 41.0 2.0 41.0 24 33.19690502199874 39.0 33.0 41.0 2.0 41.0 25 33.04927457835459 38.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7636878236117433 1101 2 3.081615991215479 1101 3 1.6924789196968106 1101 4 0.9112076953137027 1101 5 0.6683743899956305 1101 6 0.3930409728223552 1101 7 0.11350906911384584 1101 8 0.22422557349963057 1101 9 0.1976395425675932 1101 10 -0.10677336447011498 1101 11 0.10202556052323786 1101 12 -0.13426775719858597 1101 13 -0.03731641428527155 1101 14 0.053446003935590625 1101 15 0.17846150072222144 1101 16 0.1063906795443188 1101 17 -0.06750493467642826 1101 18 -0.12440001964011316 1101 19 -0.0189014394425584 1101 20 -0.038996726389513015 1101 21 0.05793251854719017 1101 22 -0.04009316511955774 1101 23 -0.03747505924130934 1101 24 -0.08250525535579811 1101 25 -0.29116079628055047 1102 1 1.269452837279772 1102 2 3.5904431350206245 1102 3 2.350269969527087 1102 4 1.5028875384522387 1102 5 1.1158465555521992 1102 6 1.1006334880401596 1102 7 0.9469424890159175 1102 8 0.8762959863806259 1102 9 0.8357975127628237 1102 10 0.8893638128428663 1102 11 0.9308238662883852 1102 12 0.7590342256897458 1102 13 0.7794465157251267 1102 14 0.754238367316745 1102 15 0.7407567192331825 1102 16 0.7944373665833169 1102 17 0.5139984393196357 1102 18 0.6790575411969542 1102 19 0.6687074883339861 1102 20 0.8300135772018606 1102 21 0.7793629235632622 1102 22 0.7468969463820656 1102 23 0.67898310511913 1102 24 0.5222699526686085 1102 25 0.5348958475286949 1103 1 1.0867219626345168 1103 2 2.791704259986645 1103 3 1.917787498284227 1103 4 1.1646982895983875 1103 5 1.0290003135154855 1103 6 0.8838657659052629 1103 7 0.7118064416964813 1103 8 0.6136015416078635 1103 9 0.7169038830001213 1103 10 0.6696853780403913 1103 11 0.6023928066045983 1103 12 0.5982581932364326 1103 13 0.6888023215883194 1103 14 0.7632164791882303 1103 15 0.5867286180647753 1103 16 0.57342662804448 1103 17 0.7661125932575175 1103 18 0.6074703465466555 1103 19 0.7077793802052952 1103 20 0.8294708062464764 1103 21 0.8514540166301643 1103 22 0.8104228169759082 1103 23 0.890295013905245 1103 24 0.7212075241592757 1103 25 0.7893160885647035 1104 1 1.3812516352887911 1104 2 3.852855104279268 1104 3 2.4619869464321056 1104 4 1.8597230098489455 1104 5 1.3441741906300742 1104 6 1.105700552123615 1104 7 0.9778104745608545 1104 8 0.9500206973280925 1104 9 1.0596016096623941 1104 10 1.2030367037578031 1104 11 1.119550087030035 1104 12 1.1498196108820764 1104 13 0.9977043106463768 1104 14 1.1635114257701318 1104 15 0.9317895158590375 1104 16 1.00842682724587 1104 17 1.0469246152544898 1104 18 1.0372605939033832 1104 19 1.0425734380919351 1104 20 0.8841246006475032 1104 21 1.0577923479907625 1104 22 1.0277654189573866 1104 23 1.0986493919021 1104 24 1.0716850291810758 1104 25 1.0672297945203084 1105 1 1.1822426399864838 1105 2 3.4478057888351117 1105 3 2.1464492474939227 1105 4 1.5169619836068584 1105 5 1.0808554518882936 1105 6 0.7229946735273245 1105 7 0.3726867774146001 1105 8 0.45640005527876326 1105 9 0.5562084140854751 1105 10 0.6853066229334459 1105 11 0.5121870498523364 1105 12 0.4425818580359433 1105 13 0.5242523798213625 1105 14 0.5217896771990667 1105 15 0.6691514823910296 1105 16 0.5386624256522978 1105 17 0.7621749303436474 1105 18 0.6633186078743663 1105 19 0.7944819110318306 1105 20 0.6843965822248848 1105 21 0.6780951716779526 1105 22 0.8298338313400606 1105 23 0.6990318190371454 1105 24 0.7258476809263286 1105 25 0.7499764327866885 1106 1 0.5661551365321635 1106 2 3.0349329634813103 1106 3 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0.5344749754947387 1107 15 0.5675463150203157 1107 16 0.6625018343141136 1107 17 0.497970411380976 1107 18 0.4269449681791144 1107 19 0.4233304738723973 1107 20 0.5973712706875105 1107 21 0.6887227224921872 1107 22 0.5592089537325151 1107 23 0.8403947192799137 1107 24 0.8646632638563503 1107 25 1.0253540232327722 1108 1 0.7594313282827159 1108 2 2.365726698755214 1108 3 1.4868586889548467 1108 4 0.9200987621714312 1108 5 0.8763880991490929 1108 6 0.7961367290299819 1108 7 0.7501570043558701 1108 8 0.72636449064521 1108 9 0.797210152440158 1108 10 0.5913812420846085 1108 11 0.6914616696622389 1108 12 0.6273132741209864 1108 13 0.6752623264849973 1108 14 0.6960146225064108 1108 15 0.5992916917887783 1108 16 0.658525027641538 1108 17 0.6777613303826016 1108 18 0.8245702373613213 1108 19 0.8369049172585221 1108 20 0.7953592034821213 1108 21 0.9379344967945045 1108 22 0.8805344313300338 1108 23 1.043537180787368 1108 24 1.0228188408010794 1108 25 1.0637382779599633 1109 1 1.1773041751467872 1109 2 3.354116817700035 1109 3 1.8737835187939105 1109 4 1.2904064462504259 1109 5 0.9725712071309367 1109 6 0.4865109565291519 1109 7 0.4884488995601117 1109 8 0.4595238789968974 1109 9 0.5122549919904387 1109 10 0.5123128367605219 1109 11 0.4643015782816917 1109 12 0.4062989623992195 1109 13 0.402173584389395 1109 14 0.4771612330775099 1109 15 0.35522930315028844 1109 16 0.48250643369485147 1109 17 0.5104365004554978 1109 18 0.45029398399888265 1109 19 0.39218655911484746 1109 20 0.36209191758951675 1109 21 0.5877558011221353 1109 22 0.5555679264509834 1109 23 0.5077751549549134 1109 24 0.5249458662736615 1109 25 0.5382840770763977 1110 1 -0.012052137206943314 1110 2 1.5717150455382054 1110 3 0.5601517639605404 1110 4 0.3127092253639461 1110 5 0.12256940507301906 1110 6 0.1062613199441671 1110 7 0.01121675845907788 1110 8 0.020650799902931283 1110 9 -0.14871348602972034 1110 10 -0.2537239105493896 1110 11 -0.3365089331487212 1110 12 -0.31086257205256373 1110 13 -0.1582665345017773 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-2.34237002029737 2315 24 -2.283835382974914 2315 25 -2.4053213989060644 2316 1 -1.9329904765824217 2316 2 -2.30656082201725 2316 3 -1.4883896624055808 2316 4 -2.066844262314042 2316 5 -2.1032602844704122 2316 6 -2.242008305817013 2316 7 -2.2951779808038175 2316 8 -2.532736109593248 2316 9 -2.6972531750080435 2316 10 -2.7845574232603916 2316 11 -2.967781748936787 2316 12 -2.8379446072981267 2316 13 -3.0126770946008534 2316 14 -3.2340475443722205 2316 15 -3.3150437958098387 2316 16 -3.299463770279946 2316 17 -3.1311431975122233 2316 18 -3.1030541352052836 2316 19 -3.3725524827733544 2316 20 -3.446043203151131 2316 21 -3.399652213676859 2316 22 -3.245490466468887 2316 23 -3.3397091880785332 2316 24 -3.369181589319961 2316 25 -3.7463553301611476 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 174490.0 3 2.0 4 1446.0 5 7136.0 6 8153.0 7 7391.0 8 6795.0 9 6587.0 10 6368.0 11 6023.0 12 6030.0 13 5880.0 14 5987.0 15 6190.0 16 6523.0 17 6899.0 18 8220.0 19 10091.0 20 13042.0 21 16675.0 22 20460.0 23 24069.0 24 27775.0 25 31247.0 26 35013.0 27 38728.0 28 44279.0 29 52612.0 30 64803.0 31 79891.0 32 99551.0 33 126502.0 34 163897.0 35 216215.0 36 300676.0 37 410181.0 38 804791.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.429915898938404 18.532788328706268 12.747902384675886 25.289393387679443 2 16.39394073949576 21.591592572382066 38.63202421707885 23.382442471043323 3 18.498900940076854 24.765015866734863 29.855596225099255 26.880486968089023 4 12.641258842819347 17.054196668932843 38.08314547933115 32.22139900891666 5 13.643252094808917 37.480890108741335 35.474904038352385 13.400953758097367 6 31.601849142887612 36.962055245564294 18.3420928374128 13.094002774135294 7 27.88328706266501 31.79829777262334 22.36623777721182 17.952177387499834 8 25.452368565712497 36.04295506185174 20.729199079353975 17.77547729308179 9 26.585007180899016 15.581112586814507 20.029937367967225 37.80394286431925 10 15.277038172073565 28.700618602702992 34.09471209400909 21.927631131214355 11 34.28789827328671 22.625234247450905 24.428983469549408 18.657884009712983 12 23.254922265978816 24.643884238435316 31.313981740099866 20.787211755486005 13 28.668099338459243 21.822952075655174 25.864391510893427 23.64455707499216 14 21.675333235810314 21.527399934540302 27.454414126946208 29.342852702703176 15 23.424359209125882 29.56432605140359 23.584289441798234 23.427025297672294 16 22.984524759192567 27.81782757282807 26.611141864676362 22.586505803303 17 22.38900477019369 27.90331780687556 27.238128714545407 22.46954870838534 18 22.18041842154929 27.460641867833573 28.460389992626162 21.898549717990974 19 23.72820292624882 26.898785243309153 27.161325357502182 22.211686472939846 20 23.7246515047816 27.04734034398917 27.45749773820578 21.770510413023445 21 23.557593476221648 26.543437247642444 26.948437145910116 22.950532130225795 22 23.564987325484232 27.089400765171707 27.114728748523998 22.230883160820063 23 23.235189186287954 26.890065014314512 27.1134914262 22.76125437319753 24 23.264502427376044 26.712116603855527 27.537203453718135 22.486177515050294 25 23.282881115603704 26.80495948597813 27.314673519917438 22.59748587850073 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 580.0 1 580.0 2 670.5 3 761.0 4 761.0 5 761.0 6 1729.5 7 2698.0 8 2698.0 9 2698.0 10 4071.0 11 5444.0 12 5444.0 13 5444.0 14 9121.5 15 12799.0 16 12799.0 17 12799.0 18 22412.5 19 32026.0 20 32026.0 21 32026.0 22 49493.0 23 66960.0 24 66960.0 25 66960.0 26 95011.5 27 123063.0 28 123063.0 29 123063.0 30 158178.5 31 193294.0 32 193294.0 33 193294.0 34 223481.5 35 253669.0 36 253669.0 37 253669.0 38 280473.5 39 307278.0 40 307278.0 41 307278.0 42 329502.5 43 351727.0 44 351727.0 45 351727.0 46 360857.5 47 369988.0 48 369988.0 49 369988.0 50 363204.5 51 356421.0 52 356421.0 53 356421.0 54 325126.0 55 293831.0 56 293831.0 57 293831.0 58 253864.5 59 213898.0 60 213898.0 61 213898.0 62 175325.0 63 136752.0 64 136752.0 65 136752.0 66 105884.5 67 75017.0 68 75017.0 69 75017.0 70 54523.0 71 34029.0 72 34029.0 73 34029.0 74 23806.0 75 13583.0 76 13583.0 77 13583.0 78 9173.0 79 4763.0 80 4763.0 81 4763.0 82 3106.0 83 1449.0 84 1449.0 85 1449.0 86 946.0 87 443.0 88 443.0 89 443.0 90 276.5 91 110.0 92 110.0 93 110.0 94 68.5 95 27.0 96 27.0 97 27.0 98 17.5 99 8.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.406027043960292 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 3.858812369808933E-4 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 3.157210120752763E-4 15 0.0 16 0.0 17 0.0 18 0.0 19 2.104806747168509E-4 20 1.754005622640424E-4 21 0.0 22 5.612817992449357E-4 23 5.262016867921272E-4 24 3.157210120752763E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2850618.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.67908073926061 #Duplication Level Percentage of deduplicated Percentage of total 1 84.66912916977411 63.23012733392447 2 10.377536561285655 15.499697814697608 3 2.564614885359526 5.745692462666209 4 0.9322159136801903 2.784681099365862 5 0.47542179029397963 1.7752031131283967 6 0.25843570527762066 1.1579844540201116 7 0.17643047783688237 0.9222966129472808 8 0.11591780609409891 0.6925308160335335 9 0.0761947995079166 0.5121141825927131 >10 0.3283370314749266 4.111097350277937 >50 0.014922460237930701 0.7759052168380344 >100 0.009186753701197606 1.3247878451556085 >500 0.0010413200136506076 0.5509991070349987 >1k 6.153254623849716E-4 0.9168825913173506 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 4971 0.17438323900291094 No Hit GGTATCAACGCAGAGTACTTTTTTT 3007 0.10548589814559509 No Hit TATCAACGCAGAGTACTTTTTTTTT 2942 0.10320569083616254 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.508011245280848E-5 0.0 3 0.0 0.0 0.0 3.508011245280848E-5 0.0 4 0.0 0.0 0.0 3.508011245280848E-5 0.0 5 0.0 0.0 0.0 3.508011245280848E-5 3.508011245280848E-5 6 0.0 0.0 0.0 3.508011245280848E-5 3.508011245280848E-5 7 0.0 0.0 0.0 3.508011245280848E-5 3.508011245280848E-5 8 3.508011245280848E-5 0.0 0.0 7.016022490561696E-5 3.508011245280848E-5 9 3.508011245280848E-5 0.0 0.0 1.4032044981123393E-4 3.508011245280848E-5 10 7.016022490561696E-5 0.0 0.0 1.4032044981123393E-4 3.508011245280848E-5 11 7.016022490561696E-5 0.0 0.0 1.4032044981123393E-4 3.508011245280848E-5 12 7.016022490561696E-5 0.0 0.0 1.4032044981123393E-4 7.016022490561696E-5 13 7.016022490561696E-5 3.508011245280848E-5 0.0 1.7540056226404238E-4 7.016022490561696E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1240 0.0 12.683907 1 GTATCAA 2790 0.0 10.7427635 1 TATGTCG 115 9.3700146E-7 10.689203 16 TCCAACG 160 5.9117156E-10 10.638 18 TGGACGG 110 6.3955886E-6 10.315455 5 AAGGCGT 205 3.6379788E-12 10.147724 6 CCAACGA 140 1.037879E-7 10.131429 19 CGTATAG 75 0.0027526962 10.0864 10 GTAGGAC 1495 0.0 9.993457 3 GTCCTAC 1525 0.0 9.859419 1 TAGGACC 620 0.0 9.760861 4 TGTAGGA 1645 0.0 9.440856 2 GTATAAA 500 0.0 9.29834 1 GTACTAG 150 1.5626283E-6 9.232395 1 TAGGACG 1345 0.0 9.139468 4 GTCCTAT 315 0.0 9.106783 1 GGCGAGG 615 0.0 9.0716095 19 AGGACGT 1230 0.0 9.07145 5 GGACGTG 1225 0.0 8.876905 6 GTGCGCC 160 7.212184E-7 8.865 11 >>END_MODULE