FastQCFastQC Report
Thu 26 May 2016
SRR1512109_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512109_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2850618
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT64900.22766992981872702No Hit
TATCAACGCAGAGTACTTTTTTTTT41320.14495102465500465No Hit
GGTATCAACGCAGAGTACTTTTTTT40690.1427409775704777No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC6100.011.9943074
GGTATCA15300.011.9761851
CCGACCA700.001496898110.8529339
GGCGAGG7000.010.17480419
CCAACGA1502.4614565E-810.12958219
GTATCAA31450.09.9835251
GTAGGAC16150.09.8844173
TTATACT3200.09.7989214
TAGGGTG2054.1836756E-119.7337385
TATACTG4700.09.7041535
TGTAGGA17600.09.60996152
GGACCGT1106.8691406E-59.4963176
CGTGCGC1002.767405E-49.49631610
GGACGTG13650.09.4615326
AGGACGT14000.09.4341135
TGGCGAG14850.09.2726618
TCTAGTA2054.2200554E-109.2702272
TCCAACG1951.6952981E-99.25298318
ATAGGAC2800.08.823273
GTATAAA4550.08.8094041
Produced by FastQC (version 0.11.5)