FastQCFastQC Report
Thu 26 May 2016
SRR1512108_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512108_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174758
Sequences flagged as poor quality0
Sequence length25
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT50772.9051602787855204No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA40922.3415237070692045No Hit
TATCAACGCAGAGTACTTTTTTTTT35492.0308083177880265No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT33021.889470009956626No Hit
GGTATCAACGCAGAGTACTTTTTTT32461.8574256972499112No Hit
GTACATAAGCAGTGGTATCAACGCA20361.1650396548369746No Hit
GTACTTTTTTTTTTTTTTTTTTTTT17931.0259902264846246No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17220.9853626157314687No Hit
GGTATCAACGCAGAAAAAAAAAAAA16840.963618260680484No Hit
GAGTACTTTTTTTTTTTTTTTTTTT12720.7278636743382277No Hit
GGTATCAACGCAGAGAAAAAAAAAA10650.6094141612973369No Hit
GTATCAACGCAGAGAAAAAAAAAAA9630.5510477345815357No Hit
GCTTATGTACTCTGCGTTGATACCA8080.46235365476830814No Hit
GTATCAACGCAGAAAAAAAAAAAAA7860.44976481763352755No Hit
GCAGAGTACTTTTTTTTTTTTTTTT6470.3702262557365042No Hit
ATCAACGCAGAGTACTTTTTTTTTT5740.32845420524382285No Hit
TATCAACGCAGAGAAAAAAAAAAAA5380.3078542899323636No Hit
CTTATGTACTCTGCGTTGATACCAC5270.30155987136497325No Hit
GAGTACATAAGCAGTGGTATCAACG5240.299843211755685No Hit
GTGGTATCAACGCAGAGTACATGGG4540.25978782087229196No Hit
GTGGTATCAACGCAGAGTACTTTTT4180.2391879055608327No Hit
GAAAAAAAAAAAAAAAAAAAAAAAA3990.22831572803534028No Hit
CATAAGCAGTGGTATCAACGCAGAG3740.21401023129127136No Hit
TATGTACTCTGCGTTGATACCACTG3710.21229357168198307No Hit
TATCAACGCAGAAAAAAAAAAAAAA3610.20657137298435552No Hit
GGTATCAACGCAGAGTACATGGGGG3450.1974158550681514No Hit
GCGTTGATACCACTGCTTATGTACT3200.18311035832408246No Hit
ANAAAAAAAAAAAAAAAAAAAAAAA2960.16937708144977626No Hit
GTACTGGTTCACTATCGGTCAGTCA2890.16537154236143695No Hit
GNATCAACGCAGAGTACTTTTTTTT2750.15736046418475835No Hit
GTATCAACGCAGAGTACATAAGCAG2750.15736046418475835No Hit
GGTATCAACGCAGAGTACATGGGGA2660.15221048535689355No Hit
TATCAACGCAGAGTACATAAGCAGT2660.15221048535689355No Hit
TNTTTTTTTTTTTTTTTTTTTTTTT2510.14362718731045215No Hit
GTGGTATCAACGCAGAAAAAAAAAA2410.1379049886128246No Hit
CAACGCAGAGTACTTTTTTTTTTTT2350.13447166939424804No Hit
ACGCAGAGTACATAAGCAGTGGTAT2260.12932169056638324No Hit
GTATCAACGCAGAGTACATGGGGGG2250.12874947069662046No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT2230.12760503095709497No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT2220.12703281108733222No Hit
GGTATCAACGCAGAGTACATAAGCA2190.12531615147804392No Hit
CTGCTTATGTACTCTGCGTTGATAC2100.12016617265017912No Hit
GAATCAACGCAGAGTACTTTTTTTT2040.11673285343160257No Hit
GNTATCAACGCAGAGTACTTTTTTT2020.11558841369207704No Hit
TNTCAACGCAGAGTACTTTTTTTTT1950.11158287460373774No Hit
GTACATGGGGTGGTATCAACGCAGA1930.11043843486421223No Hit
AACGCAGAGTACTTTTTTTTTTTTT1890.10814955538516119No Hit
GTATCAACGCAGAGTACATGGGGTG1870.10700511564563568No Hit
TCAACGCAGAGTACTTTTTTTTTTT1860.10643289577587291No Hit
GAGAAAAAAAAAAAAAAAAAAAAAA1780.10185513681777085No Hit
ACTCTGCGTTGATACCACTGCTTAT1760.10071069707824534No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATCAA557.339513E-616.2478071
TTACAGG350.002234283616.203313
CATCAAC601.6742415E-514.8938232
GTTATCA400.003916735314.8938231
GAATCAA704.539219E-614.1845941
AAGCAGA450.00855088513.2389551
GGGATCA450.00855088513.2389551
GGACATG600.00419953811.58408451
GACATGG650.007621555610.6930022
AATCAAC951.0387088E-410.4518052
CGCAGAA3650.010.358289
GCAGAAA3700.010.21830510
GGTATCA18350.09.848051
CAGAAAA3850.09.820188511
GGGGAGG1304.561795E-58.724867
GCTTATG1904.5504748E-78.3614441
TGGGGAG1752.5588524E-68.1016556
TTATGTA2855.2750693E-117.95952133
AGCAGTG5600.07.9328718
ACATAAG5300.07.8468863
Produced by FastQC (version 0.11.5)