##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512108_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 174758 Sequences flagged as poor quality 0 Sequence length 25 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.14312935602376 31.0 28.0 33.0 16.0 34.0 2 25.352447384382977 31.0 22.0 33.0 2.0 34.0 3 26.94908387598851 31.0 25.0 33.0 16.0 34.0 4 31.326760434429325 35.0 32.0 37.0 19.0 37.0 5 31.791591801233707 35.0 32.0 37.0 19.0 37.0 6 32.144222295975005 35.0 33.0 37.0 19.0 37.0 7 32.22991222147198 35.0 33.0 37.0 21.0 37.0 8 32.247542315659366 35.0 33.0 37.0 22.0 37.0 9 33.60198102518912 38.0 34.0 39.0 22.0 39.0 10 33.480470135844996 38.0 33.0 39.0 20.0 39.0 11 33.454760297096556 38.0 33.0 39.0 19.0 39.0 12 33.35518831755914 38.0 33.0 39.0 18.0 39.0 13 33.21226496068849 38.0 33.0 39.0 17.0 39.0 14 34.051310955721625 38.0 33.0 41.0 17.0 41.0 15 33.851920942102794 38.0 33.0 41.0 17.0 41.0 16 33.83898305084746 38.0 33.0 41.0 17.0 41.0 17 34.012817725082684 38.0 33.0 40.0 17.0 41.0 18 33.95005092756841 38.0 33.0 40.0 17.0 41.0 19 34.01440849632063 39.0 34.0 41.0 18.0 41.0 20 33.994340745488046 39.0 34.0 41.0 18.0 41.0 21 33.92780874123073 39.0 34.0 41.0 16.0 41.0 22 33.76187642339693 38.0 33.0 41.0 10.0 41.0 23 33.57874317627805 38.0 33.0 41.0 8.0 41.0 24 33.401818514746104 38.0 33.0 40.0 2.0 41.0 25 33.243719886929355 38.0 33.0 40.0 2.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -0.31584704005332753 1101 2 1.3984771732169747 1101 3 0.5230514683363445 1101 4 -0.7128008448266456 1101 5 -0.9913127525785228 1101 6 -1.2511953635570379 1101 7 -1.0966807203837874 1101 8 -0.9787589084103452 1101 9 -1.0513974384274434 1101 10 -0.7939565597474783 1101 11 -0.9255407115159784 1101 12 -1.6356993485457458 1101 13 -1.4251582418448479 1101 14 -1.069915725951347 1101 15 -1.543097326842883 1101 16 -1.5160957686774168 1101 17 -2.0072244127828824 1101 18 -2.0896493078025813 1101 19 -2.0535602686787584 1101 20 -2.148034313740073 1101 21 -2.6397438808893945 1101 22 -2.3383734957987627 1101 23 -2.217112956769924 1101 24 -2.6778522144682384 1101 25 -2.1775722153039005 1102 1 1.358654484694501 1102 2 3.5538137906225558 1102 3 2.241559561228673 1102 4 1.691843771640631 1102 5 1.0985300811066772 1102 6 1.055020916058261 1102 7 0.6490851721801363 1102 8 0.5878843005973948 1102 9 0.8984031714716849 1102 10 0.7121658892321179 1102 11 0.7728407100181514 1102 12 0.5153679749353728 1102 13 0.6032724407730257 1102 14 0.799049791290031 1102 15 1.076841683243913 1102 16 1.0352530467108068 1102 17 0.684964186794879 1102 18 -0.16466690104677895 1102 19 0.5911969445513634 1102 20 0.44399008222521275 1102 21 0.6270166164129733 1102 22 0.3821988710790194 1102 23 0.034540808184331695 1102 24 0.16066526148766513 1102 25 0.5139595698239461 1103 1 0.9851805918260972 1103 2 2.7594271617988433 1103 3 1.9327340442669225 1103 4 1.0782473396903676 1103 5 0.761142145800104 1103 6 0.6960985391604808 1103 7 0.6707547671704504 1103 8 0.4509739073010053 1103 9 0.8615963957812767 1103 10 0.935844764755835 1103 11 0.1472156255472754 1103 12 0.41476879486599216 1103 13 0.3995962683172891 1103 14 0.24938091711784693 1103 15 0.20122786155629768 1103 16 0.1537375265954779 1103 17 0.544134344924359 1103 18 0.8170873900736453 1103 19 0.8162273540661644 1103 20 0.6207256771254208 1103 21 1.0267553198413637 1103 22 0.7528345425661627 1103 23 1.0012930720037687 1103 24 0.18828160603872846 1103 25 0.2592855147714559 1104 1 1.9836992394385042 1104 2 4.545981710422055 1104 3 3.386027874869921 1104 4 2.9298942549918685 1104 5 2.204240786441442 1104 6 2.1372801355355193 1104 7 1.8815860672750162 1104 8 2.2550432694480946 1104 9 1.9760526523166604 1104 10 2.3863065981472644 1104 11 1.8725990344005368 1104 12 1.8953805062636917 1104 13 2.5658127200426293 1104 14 2.700365580763716 1104 15 2.1934725461153732 1104 16 2.547515846567592 1104 17 2.138374498287895 1104 18 2.3768207466438795 1104 19 2.2439896405771407 1104 20 2.37233773707662 1104 21 2.5263631971505305 1104 22 2.077642838139532 1104 23 2.2070703463153762 1104 24 2.0847612156588013 1104 25 2.228961360013706 1105 1 0.6772124811414884 1105 2 2.627820463355345 1105 3 1.5658803723556716 1105 4 1.4202982107411124 1105 5 0.7236004914364109 1105 6 0.46270755759385196 1105 7 0.23303594994347065 1105 8 -0.190355657806343 1105 9 0.08697286686577144 1105 10 0.1448969434372387 1105 11 0.02969759289869245 1105 12 0.10469160247555465 1105 13 -0.09983427962213653 1105 14 0.028349154347353078 1105 15 0.1052224623071254 1105 16 0.15453897764368918 1105 17 -0.054929222798257626 1105 18 -0.09070355774767336 1105 19 0.6895980884484132 1105 20 -0.21704375806249487 1105 21 0.2728851549111795 1105 22 0.5377082085719778 1105 23 0.47052156991577476 1105 24 0.5550930963158649 1105 25 -0.22896690012709087 1106 1 1.0933483622455213 1106 2 3.007542451898388 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-2.417507386882029 2315 25 -2.2896411808211425 2316 1 -1.643210777539423 2316 2 -2.0350878990855854 2316 3 -1.2230553169806733 2316 4 -2.3625505667398805 2316 5 -2.5652838315551634 2316 6 -2.7281697795748343 2316 7 -2.5691779395161554 2316 8 -2.560321756018798 2316 9 -2.8096009979380128 2316 10 -3.1100855920055395 2316 11 -3.5662804223057485 2316 12 -3.633057524296582 2316 13 -3.645986940398796 2316 14 -4.084014724839001 2316 15 -4.050160730624864 2316 16 -3.9717965473203556 2316 17 -3.941846214785105 2316 18 -3.5187649918960204 2316 19 -3.897776064006898 2316 20 -3.8358541135176054 2316 21 -3.778815071100741 2316 22 -3.884369046410555 2316 23 -3.2286257487610257 2316 24 -3.623430634935424 2316 25 -3.716921492278317 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9871.0 3 1.0 4 82.0 5 372.0 6 456.0 7 419.0 8 393.0 9 344.0 10 323.0 11 331.0 12 321.0 13 321.0 14 316.0 15 312.0 16 312.0 17 381.0 18 438.0 19 597.0 20 787.0 21 943.0 22 1231.0 23 1452.0 24 1641.0 25 1869.0 26 2056.0 27 2356.0 28 2717.0 29 3222.0 30 3921.0 31 5013.0 32 6672.0 33 8356.0 34 11194.0 35 15261.0 36 22499.0 37 27189.0 38 40789.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.272948877876836 18.273841540873665 14.855400038910952 16.59780954233855 2 17.633160995551282 24.321269688589638 37.93495250691355 20.110616808945533 3 19.11099921033658 31.42974856658923 31.172821845065744 18.286430378008443 4 11.216653887089576 20.842536536238683 34.83216791219858 33.10864166447316 5 10.30453541468774 41.07852001052885 32.352739216516554 16.26420535826686 6 25.40885109694549 37.24979686194623 19.58193616315133 17.759415877956947 7 22.349191453324025 40.375261790590415 20.436260428707126 16.83928632737843 8 26.19737007747857 32.85800936151707 20.602776410808087 20.341844150196273 9 29.253024182011693 16.4049714462285 19.552180729923666 34.789823641836136 10 20.11982284072832 29.21239657125854 26.449146820174185 24.218633767838956 11 28.8135593220339 28.062806852905158 20.769864612778814 22.353769212282128 12 26.806212018906145 31.070394488378216 25.845454857574474 16.27793863514117 13 30.209203584385264 25.376806784238777 27.08144977626203 17.33253985511393 14 25.12045228258506 27.456253790956637 27.908307488069216 19.514986438389087 15 25.703544329873314 29.690200162510443 27.88541869327871 16.72083681433754 16 18.590851348722236 32.86201490060541 28.742031838313554 19.805101912358804 17 17.575161079893338 33.22365785829547 28.10229002391879 21.0988910378924 18 16.586365144943294 28.78323166893647 35.805513910665034 18.8248892754552 19 18.628152234245267 30.614510434489034 34.29562192072421 16.461715410541494 20 19.681502420490048 28.880509046796142 35.104544570205654 16.333443962508156 21 20.687464951532977 29.806360796072283 33.40734043648932 16.098833815905422 22 19.647281653953776 28.964791109941235 33.257609137259166 18.130318098845827 23 20.41634955967429 28.413740222137022 33.787487768730294 17.38242244945839 24 18.17609595037681 29.70353118902247 33.31998145997013 18.800391400630588 25 18.524473843829753 31.22661051282345 33.73350576225409 16.51540988109271 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 8234.0 1 8234.0 2 4981.0 3 1728.0 4 1728.0 5 1728.0 6 2186.5 7 2645.0 8 2645.0 9 2645.0 10 2295.5 11 1946.0 12 1946.0 13 1946.0 14 1593.5 15 1241.0 16 1241.0 17 1241.0 18 2214.0 19 3187.0 20 3187.0 21 3187.0 22 5020.5 23 6854.0 24 6854.0 25 6854.0 26 9670.0 27 12486.0 28 12486.0 29 12486.0 30 13076.5 31 13667.0 32 13667.0 33 13667.0 34 12580.0 35 11493.0 36 11493.0 37 11493.0 38 11282.5 39 11072.0 40 11072.0 41 11072.0 42 15393.0 43 19714.0 44 19714.0 45 19714.0 46 19502.0 47 19290.0 48 19290.0 49 19290.0 50 19506.0 51 19722.0 52 19722.0 53 19722.0 54 17604.5 55 15487.0 56 15487.0 57 15487.0 58 12970.0 59 10453.0 60 10453.0 61 10453.0 62 9030.0 63 7607.0 64 7607.0 65 7607.0 66 5894.0 67 4181.0 68 4181.0 69 4181.0 70 3233.5 71 2286.0 72 2286.0 73 2286.0 74 1633.0 75 980.0 76 980.0 77 980.0 78 669.0 79 358.0 80 358.0 81 358.0 82 225.0 83 92.0 84 92.0 85 92.0 86 58.5 87 25.0 88 25.0 89 25.0 90 16.0 91 7.0 92 7.0 93 7.0 94 4.5 95 2.0 96 2.0 97 2.0 98 1.5 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 4.816946863662894 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 5.722198697627577E-4 20 0.0 21 0.0 22 5.722198697627577E-4 23 5.722198697627577E-4 24 5.722198697627577E-4 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 174758.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.742924501310384 #Duplication Level Percentage of deduplicated Percentage of total 1 80.56606314346224 37.6589340688266 2 11.256381064306439 10.523123404937113 3 3.774162351414546 5.292461575435746 4 1.6085790884718498 3.007587635473054 5 0.7614430692766291 1.7796037949621764 6 0.46151774456155814 1.2943613454033578 7 0.2680965147453083 0.8772130603463074 8 0.17628263983253148 0.6591972899666968 9 0.1358845348709097 0.5716476498929949 >10 0.7638914392743031 6.915849345952689 >50 0.11017664989533218 3.707412536192907 >100 0.09426224491045086 8.139827647375228 >500 0.009793479990696194 3.0711040410167203 >1k 0.012241849988370242 13.596516325432884 >5k 0.0012241849988370243 2.9051602787855204 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5077 2.9051602787855204 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 4092 2.3415237070692045 No Hit TATCAACGCAGAGTACTTTTTTTTT 3549 2.0308083177880265 No Hit TTTTTTTTTTTTTTTTTTTTTTTTT 3302 1.889470009956626 No Hit GGTATCAACGCAGAGTACTTTTTTT 3246 1.8574256972499112 No Hit GTACATAAGCAGTGGTATCAACGCA 2036 1.1650396548369746 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1793 1.0259902264846246 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1722 0.9853626157314687 No Hit GGTATCAACGCAGAAAAAAAAAAAA 1684 0.963618260680484 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1272 0.7278636743382277 No Hit GGTATCAACGCAGAGAAAAAAAAAA 1065 0.6094141612973369 No Hit GTATCAACGCAGAGAAAAAAAAAAA 963 0.5510477345815357 No Hit GCTTATGTACTCTGCGTTGATACCA 808 0.46235365476830814 No Hit GTATCAACGCAGAAAAAAAAAAAAA 786 0.44976481763352755 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 647 0.3702262557365042 No Hit ATCAACGCAGAGTACTTTTTTTTTT 574 0.32845420524382285 No Hit TATCAACGCAGAGAAAAAAAAAAAA 538 0.3078542899323636 No Hit CTTATGTACTCTGCGTTGATACCAC 527 0.30155987136497325 No Hit GAGTACATAAGCAGTGGTATCAACG 524 0.299843211755685 No Hit GTGGTATCAACGCAGAGTACATGGG 454 0.25978782087229196 No Hit GTGGTATCAACGCAGAGTACTTTTT 418 0.2391879055608327 No Hit GAAAAAAAAAAAAAAAAAAAAAAAA 399 0.22831572803534028 No Hit CATAAGCAGTGGTATCAACGCAGAG 374 0.21401023129127136 No Hit TATGTACTCTGCGTTGATACCACTG 371 0.21229357168198307 No Hit TATCAACGCAGAAAAAAAAAAAAAA 361 0.20657137298435552 No Hit GGTATCAACGCAGAGTACATGGGGG 345 0.1974158550681514 No Hit GCGTTGATACCACTGCTTATGTACT 320 0.18311035832408246 No Hit ANAAAAAAAAAAAAAAAAAAAAAAA 296 0.16937708144977626 No Hit GTACTGGTTCACTATCGGTCAGTCA 289 0.16537154236143695 No Hit GNATCAACGCAGAGTACTTTTTTTT 275 0.15736046418475835 No Hit GTATCAACGCAGAGTACATAAGCAG 275 0.15736046418475835 No Hit GGTATCAACGCAGAGTACATGGGGA 266 0.15221048535689355 No Hit TATCAACGCAGAGTACATAAGCAGT 266 0.15221048535689355 No Hit TNTTTTTTTTTTTTTTTTTTTTTTT 251 0.14362718731045215 No Hit GTGGTATCAACGCAGAAAAAAAAAA 241 0.1379049886128246 No Hit CAACGCAGAGTACTTTTTTTTTTTT 235 0.13447166939424804 No Hit ACGCAGAGTACATAAGCAGTGGTAT 226 0.12932169056638324 No Hit GTATCAACGCAGAGTACATGGGGGG 225 0.12874947069662046 No Hit ACTTTTTTTTTTTTTTTTTTTTTTT 223 0.12760503095709497 No Hit CTTTTTTTTTTTTTTTTTTTTTTTT 222 0.12703281108733222 No Hit GGTATCAACGCAGAGTACATAAGCA 219 0.12531615147804392 No Hit CTGCTTATGTACTCTGCGTTGATAC 210 0.12016617265017912 No Hit GAATCAACGCAGAGTACTTTTTTTT 204 0.11673285343160257 No Hit GNTATCAACGCAGAGTACTTTTTTT 202 0.11558841369207704 No Hit TNTCAACGCAGAGTACTTTTTTTTT 195 0.11158287460373774 No Hit GTACATGGGGTGGTATCAACGCAGA 193 0.11043843486421223 No Hit AACGCAGAGTACTTTTTTTTTTTTT 189 0.10814955538516119 No Hit GTATCAACGCAGAGTACATGGGGTG 187 0.10700511564563568 No Hit TCAACGCAGAGTACTTTTTTTTTTT 186 0.10643289577587291 No Hit GAGAAAAAAAAAAAAAAAAAAAAAA 178 0.10185513681777085 No Hit ACTCTGCGTTGATACCACTGCTTAT 176 0.10071069707824534 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 5.722198697627576E-4 0.0 0.0 0.0 0.0 11 5.722198697627576E-4 0.0 0.0 0.0 0.0 12 5.722198697627576E-4 0.0 0.0 0.0 0.0 13 5.722198697627576E-4 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCATCAA 55 7.339513E-6 16.247807 1 TTACAGG 35 0.0022342836 16.20331 3 CATCAAC 60 1.6742415E-5 14.893823 2 GTTATCA 40 0.0039167353 14.893823 1 GAATCAA 70 4.539219E-6 14.184594 1 AAGCAGA 45 0.008550885 13.238955 1 GGGATCA 45 0.008550885 13.238955 1 GGACATG 60 0.004199538 11.5840845 1 GACATGG 65 0.0076215556 10.693002 2 AATCAAC 95 1.0387088E-4 10.451805 2 CGCAGAA 365 0.0 10.35828 9 GCAGAAA 370 0.0 10.218305 10 GGTATCA 1835 0.0 9.84805 1 CAGAAAA 385 0.0 9.8201885 11 GGGGAGG 130 4.561795E-5 8.72486 7 GCTTATG 190 4.5504748E-7 8.361444 1 TGGGGAG 175 2.5588524E-6 8.101655 6 TTATGTA 285 5.2750693E-11 7.9595213 3 AGCAGTG 560 0.0 7.932871 8 ACATAAG 530 0.0 7.846886 3 >>END_MODULE